CN-116963614-B - Preparation method of fermented cornu Cervi Pantotrichum extract processed by three steps

Abstract

The invention relates to a preparation method of a fermented hairy antler extract, the fermented hairy antler extract prepared by the method and a method for increasing the content of sulfated glycosaminoglycan, N-acetylneuraminic acid and free amino acid of the fermented hairy antler extract, which is characterized by comprising the following steps of (1) treating crushed hairy antler under high temperature and pressure conditions; a step (2) of adding a complex enzyme of protease, cellulase, beta-glucanase and hemicellulase to the deer antler liquid obtained by adding water to the deer antler treated in the step (1) to react, a step (3) of sterilizing the deer antler liquid obtained by the reaction in the step (2), inoculating a culture solution of Bacillus coagulans (Bacillus coagulans) to the sterilized deer antler liquid to ferment and then filtering, and a step (4) of sterilizing the filtrate filtered in the step (3), concentrating and drying.

Inventors

• ZHENG RONGZHEN
• WU ZHIEN
• SHEN ZAIJIONG

Assignees

• 艾多美株式会社

Dates

Publication Date

20260505

Application Date

20211027

Priority Date

20201211

Claims (4)

1. 1. A method for preparing a fermented deer antler extract, comprising: Step (1), treating the crushed antler for 50-70 minutes at a high temperature of 115-125 ℃ and under a pressurizing condition of 2.5-3.5 bar; Step (2), adding protease in an amount of 0.4-0.6% w/v and a complex enzyme consisting of cellulase, beta-glucanase and hemicellulase in an amount of 0.4-0.6% w/v relative to the deer antler liquid, which is obtained by adding water in an amount of 4-6 times v/w to the deer antler treated in the step (1), and reacting the mixture at a temperature of 55-65 ℃ for 4-6 hours; Step (3) of sterilizing the deer antler liquid reacted in the step (2) at a temperature of 110-130 ℃ for 10-20 minutes, inoculating 1.5-2.5% v/v of Bacillus coagulans culture liquid to the sterilized deer antler liquid, fermenting at a temperature of 34-40 ℃ for 20-28 hours, filtering, and And (4) sterilizing the filtrate filtered in the step (3), concentrating and drying.
2. 2. The method for preparing a fermented deer antler extract as claimed in claim 1, wherein, And (4) sterilizing the filtrate filtered in the step (3) at the temperature of 90-100 ℃ for 5-15 minutes, concentrating and drying.
3. 3. A fermented deer antler extract, characterized by being prepared by the method of claim 1 or 2.
4. 4. A method for enhancing the content of sulfated glycosaminoglycans, N-acetylneuraminic acid and free amino acids of a fermented deer antler extract, comprising: Step (1), treating the crushed antler for 50-70 minutes at a high temperature of 115-125 ℃ and under a pressurizing condition of 2.5-3.5 bar; Step (2), adding protease in an amount of 0.4-0.6% w/v and a complex enzyme consisting of cellulase, beta-glucanase and hemicellulase in an amount of 0.4-0.6% w/v relative to the deer antler liquid, which is obtained by adding water in an amount of 4-6 times v/w to the deer antler treated in the step (1), and reacting the mixture at a temperature of 55-65 ℃ for 4-6 hours; Step (3) of sterilizing the deer antler liquid reacted in the step (2) at a temperature of 110-130 ℃ for 10-20 minutes, inoculating 1.5-2.5% v/v of Bacillus coagulans culture liquid to the sterilized deer antler liquid, fermenting at a temperature of 34-40 ℃ for 20-28 hours, filtering, and And (4) sterilizing the filtrate filtered in the step (3) at the temperature of 90-100 ℃ for 5-15 minutes, concentrating and drying.

Description

Preparation method of fermented cornu Cervi Pantotrichum extract processed by three steps Technical Field The present invention relates to a method for producing a fermented deer antler extract, which comprises the steps of high temperature and pressure treatment, enzyme treatment, fermentation, etc., and further comprises the steps of producing a fermented deer antler extract, and further comprises the steps of producing a sulfated glycosaminoglycan, N-acetylneuraminic acid, and free amino acids. Background Deer horn (Antler, cervi Parvum Cornu) is a young horn of deer, which has not been ossified, and has been the most preferred tonic material for people in regions centered on korea, china and japan since ancient times, together with ginseng. The medicinal material having such a tonic effect is characterized by exhibiting efficacy not selectively in specific organs but in a systemic complex. The pharmacological effects of cornu Cervi Pantotrichum include protein and nucleic acid synthesis promotion, hematopoiesis promotion, immunity enhancement, cardiovascular system nutrition, stress resistance, aging resistance, gastric ulcer resistance, etc., and the pharmacological effects of cornu Cervi Pantotrichum include ganglioside (ganglioside), pantocrine (pantocrin), amino acids, calcium phosphate, calcium carbonate, collagen, phospholipid, chondroitin (chondrin), glucosamine (glucosamine), hyaluronic acid (hyaluronic acid), etc. Sialic acid (SIALIC ACID), which is known as a functional and index substance of deer antler, is a monosaccharide that is attached to cells or water-soluble proteins in the form of sugar chains, and is a structural substance that plays an important role in neurotransmitter and ganglion cell structures of the brain, and in the structure and function of gangliosides, and among these, N-acetylneuraminic acid (N-Acetylneuraminic acid) is a representative structural component. Glycosaminoglycans (GAGs) are mucopolysaccharides, having a backbone that is partially sulfonated in the repeat of uronic acid and glucosamine, and exist in the form of proteoglycans (proteoglycan) in the organism. S-GAG is a main component of articular cartilage, nucleus pulposus of cartilage plate, etc., and is known to have an arthritis therapeutic effect, a powerful anti-inflammatory effect, a moisture retaining effect, etc. Bacillus coagulans (Bacillus coagulans) is a gram-positive facultative anaerobic bacillus and breeds in the range of 30-55 ℃ with an optimal breeding temperature of 50 ℃. Has both sporulation and lactic acid (LACTIC ACID) producing Bacillus and Lactobacillus properties. Bacillus coagulans has been attracting attention as a strain producing L-lactic acid, thermostable enzymes, and antibacterial peptide coagulants, because biological materials can be produced at high temperatures, and thus has an advantage in that contamination problems can be minimized. Until now, studies on deer antler have been reported, which focus on the analysis and exploration of various index substances contained in deer antler by extracting deer antler with water-soluble and organic solvents (alcohols, chloroform, ethanol, etc.), or extracting the active ingredients of deer antler with proteolytic enzymes and microorganisms. Korean patent No. 1776686 discloses a method for preparing a solid preparation of a fermented product of deer antler, and korean patent No. 1336292 discloses a method for extracting active ingredients from deer antler by an ultra-high pressure low temperature extraction method, but is different from the preparation method of the fermented deer antler extract processed through three steps of the present invention. Disclosure of Invention Technical problem The present invention has been made in view of the above-described circumstances, and an object of the present invention is to provide a method for producing a fermented deer antler extract containing a large amount of sulfated glycosaminoglycans, N-acetylneuraminic acid and free amino acids by optimizing production conditions such as enzyme treatment and fermentation in order to produce a fermented deer antler extract with an improved active ingredient. Technical proposal In order to solve the problems, the invention provides a preparation method of a fermented antler extract, which is characterized by comprising the steps of (1) treating crushed antler under high temperature and pressure, (2) adding protease, cellulase, beta-glucanase and hemicellulase into the antler liquid treated in the step (1) to react, (3) sterilizing the antler liquid reacted in the step (2), inoculating bacillus coagulans culture solution into the sterilized antler liquid to ferment, filtering, and (4) sterilizing, concentrating and drying the filtrate filtered in the step (3). Also, the present invention provides a fermented deer antler extract prepared by the above method. The invention also provides a method for increasing the contents of sulfated glycosaminoglycans, N