CN-116998485-B - Application of fungal metabolite crude extract 101H12 in preparation of mosquito killing agent

Abstract

The invention relates to the technical field of biology, in particular to application of a fungal metabolite crude extract 101H12 in preparation of a mosquito killer. The semilethal concentration LC 50 of 101H12 crude extract on culex reesei larvae is 31.90mg/L. Wherein, the half-lethal concentration LC 50 of the component 3 in the crude extract on the culex reelinguis larvae is 12.13mg/L, which is the most main component in insecticidal activity, and further separating and identifying to obtain 2 monomer compounds vermistatin and dihydrovermistatin, and the half-lethal concentration LC 50 of the 2 compounds on the culex reelinguis larvae is 28.13mg/L and 83.87mg/L respectively. The fungal crude extract 101H12 and the monomeric compounds vermistatin and dihydrovermistatin of the present invention may be used to prepare pesticides.

Inventors

• CHEN JUNHUI
• GUAN LIMEI
• JIN LIANG
• XU ZHIYONG
• YANG JIAN
• LI JIANGHUAI
• YANG FEIYING

Assignees

• 江西省科学院生物资源研究所

Dates

Publication Date

20260505

Application Date

20230807

Claims (1)

1. 1. The application of the monomer compound vermistatin and the monomer compound dihydrovermistatin in the preparation of the mosquito killer is characterized in that the structural formula of the monomer compound vermistatin is shown as the formula (I): the structural formula of the monomer compound dihydrovermistatin is shown as a formula (II):

Description

Application of fungal metabolite crude extract 101H12 in preparation of mosquito killing agent Technical Field The invention relates to the technical field of biology, in particular to application of a fungal metabolite crude extract 101H12 in preparation of a mosquito killer. Background Mosquitoes are an important vehicle for the transmission of a variety of serious human and animal diseases (such as malaria, dengue fever) and pose a threat to public health. Vector control is considered to be an effective way to reduce mosquito bite transmission cases. Because of the disadvantages of residue, pollution and the like of synthetic pesticides, development of novel pesticides is urgently needed. Entomopathogenic fungi are widely used in controlling pests and their extracts have the potential to develop pesticides or mosquitocides. Disclosure of Invention The invention aims to overcome the defects of the prior art, and aims to provide an application of a fungal metabolite crude extract 101H12 in preparing a mosquito killer. In order to achieve the purposes of the application, the technical scheme adopted by the invention is that in the first aspect, the invention provides application of a fungal metabolite crude extract 101H12 in preparing a mosquito killer, and in the second aspect, the invention provides the mosquito killer. The invention relates to application of a crude fungal metabolite extract 101H12 in preparing a mosquito killer, wherein the gene sequence of the crude fungal metabolite extract 101H12 is a nucleotide sequence shown as SEQ ID No. 1. Further, the crude fungal metabolite extract includes monomeric compounds vermistatin and dihydrovermistatin. Further, the structural formula (I) of vermistatin is shown as follows: Still further, the dihydrovermistatin has the structural formula (II): Further, the crude extract 101H12 of the fungal metabolite is added into the breeding water body of mosquito larvae, and the semi-lethal concentration LC 50 of the crude extract on the culex tiredness larvae is 31.90mg/L. Further, component 3 of the crude extract 101H12 of the fungal metabolite is added into the mosquito larva breeding water body, and the semi-lethal concentration LC 50 of the component 3 on the culex tiredness larva is 12.13mg/L. Further, the monomeric compound vermistatin of component 3 of the crude fungal metabolite 101H12 was added to the mosquito larval breeding water at a semi-lethal concentration LC 50 of 28.13mg/L for the culex tiredness larvae of vermistatin. Further, the mosquito is culex tiredness. The invention relates to a mosquito killer, which comprises the fungal metabolite crude extract 101H12. Further, monomer compounds vermistatin and dihydrovermistatin are also included. Advantageous effects the crude fungal extract 101H12 and the monomer compounds vermistatin and dihydrovermistatin of the present invention can be used for preparing pesticides. Compared with the prior art, the invention has the advantages that (1) the invention adopts the ethyl acetate extraction method to screen the fungal crude extract 101H12 with the best efficacy from the fungal library, and the biological assay result shows that the fungal crude extract has higher toxicity to the culex tiredness larva, in particular to the component 3 thereof. The results of the invention show the potential of fungal ethyl acetate crude extract in mosquito control. (2) After discovery of these candidate metabolites, the present invention isolates active compounds and verifies their larvicidal activity. The results of the research of the present invention show that vermistatin and dihydrovermistatin have potential as novel pesticides. Drawings In order to more clearly illustrate the technical solutions of the embodiments of the present application, the drawings that are needed in the embodiments or the description of the prior art will be briefly introduced below, and it is obvious that the drawings in the following description are only some embodiments of the present application, and that other drawings can be obtained according to these drawings without inventive effort for a person skilled in the art. FIG. 1 shows the toxicity of crude extracts 101H12 of fungal metabolites at different concentrations to culex larvae according to the present invention. FIG. 2 shows the toxicity of 4 components of crude fungal metabolite 101H12 of the present invention to culex larvae. FIG. 3 shows the toxicity of component 3 of crude fungal metabolite 101H12 at different concentrations according to the invention on culex larvae. FIG. 4 is a toxicity assay of monomeric compounds vermistatin and dihydrovermistatin of the invention on culex larvae. Detailed Description The following description of the embodiments of the present invention will be made clearly and completely with reference to the accompanying drawings, in which it is apparent that the embodiments described are only some embodiments of the present invention, but not all embodi