CN-116999456-B - Application of black corn polysaccharide in preparation of medicine for preventing or treating senile osteoporosis

Abstract

The invention discloses an application of black corn polysaccharide in preparing a medicament for preventing or treating senile osteoporosis. The evaluation of the prevention or treatment effect of the black corn polysaccharide on senile osteoporosis comprises the improvement effect on serum bone metabolic markers, bone microstructures and bone biomechanics. The black corn polysaccharide of the invention is polysaccharide powder obtained by extracting dry black corn grains with water, precipitating with ethanol, deproteinizing by Sevag method, depigmenting by H 2 O 2 method, and dialyzing by dialysis bag. The black corn polysaccharide has an improvement effect on unbalance of serum bone metabolic markers of senile osteoporosis, deterioration of bone microstructure and reduction of bone strength. The result shows that the black corn polysaccharide has a great improvement effect on bone loss in the aspect of preventing senile osteoporosis. Therefore, the black corn polysaccharide has important significance in developing senile osteoporosis clinically.

Inventors

• LIU BING
• PAN JINGHUA
• CHEN JUN
• LIU SHIYUN
• XU YING
• ZENG CHENG
• YOU TIANHUI

Assignees

• 广东药科大学

Dates

Publication Date

20260508

Application Date

20230830

Claims (3)

1. 1. The black corn polysaccharide is polysaccharide powder obtained by extracting dry black corn grains with water, precipitating with ethanol, deproteinizing by Sevag method, depigmenting by H 2 O 2 method, and dialyzing by dialysis bag; the preparation method of the black corn polysaccharide comprises the following steps: (1) Weighing sun-dried black corn grains, adding ethanol, soaking overnight, drying to constant weight, and pulverizing; (2) Adding deionized water, performing ultrasonic extraction, centrifuging, and collecting supernatant, wherein the ultrasonic extraction condition is 70 ℃ and 100W; (3) Performing rotary evaporation on the supernatant to obtain a concentrated solution; (4) Adding ethanol to precipitate protein, stirring, refrigerating, and standing overnight; (5) Centrifuging, collecting precipitate, dissolving the precipitate with deionized water, steaming, and lyophilizing to obtain black corn crude polysaccharide; (6) Preparing the crude polysaccharide into a solution, deproteinizing by a Sevag method, depigmenting by an H 2 O 2 method, dialyzing by a dialysis bag, and freeze-drying to obtain the black corn polysaccharide.
2. 2. The use according to claim 1, wherein in step (2) and step (5), the centrifugation conditions for the centrifugation are 3000 r/min, 4 ℃.
3. 3. The use according to claim 1, wherein in step (2), the centrifuged precipitate is subjected to ultrasound-assisted aqueous extraction twice repeatedly, and the supernatants obtained are combined and subjected to step (3).

Description

Application of black corn polysaccharide in preparation of medicine for preventing or treating senile osteoporosis Technical Field The invention relates to application of black corn polysaccharide in preparing a medicament for preventing or treating senile osteoporosis. Background Osteoporosis is one of the most common bone diseases, and is a systemic bone disease characterized by low bone mass, damaged bone tissue structure, increased bone fragility, and susceptibility to fracture. Senile osteoporosis is primary osteoporosis, is common in the elderly over 70 years old, and is a low-conversion type osteoporosis with significantly reduced osteogenesis rate due to aging. The most used drugs for clinically treating osteoporosis at present are estrogen, calcium agent, active vitamin D, calcitonin, fluoride and the like, and the drugs have certain curative effect, but have large side effect and high price. Therefore, many scholars turn the research focus to natural products with the characteristics of safety and effectiveness and obtain a certain effect, wherein the research report of plant polysaccharide extracts on senile osteoporosis is also increasing. Black corn (Zeamays l.) is also known as Black waxy corn, purple corn, a monocotyledonous class of the phylum angiosperma, a plant of the genus zea of the order gramineae. The black corn has rich nutrition, and contains protein, amino acid, fat and selenium higher than yellow corn, and contains a large amount of functional melanin and microelements Zn, fe, ca, P necessary for human body. The black corn also contains rich polysaccharide substances, and researches show that the polysaccharide is a substance with biological activity, can activate immune cells, improve the immune function of organisms, has antiviral and anti-aging effects, has great potential in the health food market, and has less research reports on senile osteoporosis. Therefore, the invention uses the black corn polysaccharide to research the aspects of bone metabolic markers, bone morphology, bone strength and the like of senile osteoporosis, and initially determines the potential of using the black corn polysaccharide to develop products for preventing senile osteoporosis. Disclosure of Invention The invention aims to solve the technical problem of providing application of black corn polysaccharide in preparing medicaments for preventing or treating senile osteoporosis. The evaluation of the preventive or therapeutic effect of the black corn polysaccharide on senile osteoporosis is actually an improvement effect on serum bone metabolic markers, bone microstructures and bone biomechanics. The black corn polysaccharide of the invention is polysaccharide powder obtained by extracting dry black corn grains with water, precipitating with ethanol, deproteinizing by Sevag method, depigmenting by H 2O2 method, and dialyzing by dialysis bag. The black corn polysaccharide has the effects of improving unbalance of serum bone metabolic markers, deterioration of bone microstructure and reduction of bone strength of senile osteoporosis. The result of the invention shows that the black corn polysaccharide has great improvement effect on bone loss in the aspect of preventing senile osteoporosis. Therefore, the black corn polysaccharide has important significance for developing senile osteoporosis clinically. Drawings FIG. 1 is a graph of the middle and upper tibial segment HE staining (x 20). Detailed Description The present invention will be further described with reference to the accompanying drawings for a clear and intuitive understanding to those skilled in the art. Example 1 preparation of Black corn polysaccharide Weighing a proper amount of sun-dried black corn grains, adding 95% ethanol for soaking overnight, drying at 50 ℃ to constant weight, pulverizing, and sieving with 60 mesh sieve. Adding deionized water according to a feed liquid ratio of 1:20 (g/ml), carrying out ultrasonic extraction at 70 ℃ and 100W for 1 hour, centrifuging at 3000r/min and 4 ℃ for 10 minutes, collecting supernatant, carrying out ultrasonic assisted water extraction on precipitate again, obtaining supernatant again, merging the supernatant, carrying out rotary evaporation to 1/10 of the volume of the supernatant to obtain concentrated solution, adding 95% ethanol with three volumes into the concentrated solution for precipitation, continuously stirring, standing at 4 ℃ for overnight, centrifuging at 3000r/min and 4 ℃ for 10 minutes, collecting precipitate, dissolving the precipitate with deionized water, carrying out rotary evaporation, and carrying out freeze drying to obtain the black corn crude polysaccharide. Preparing a proper amount of crude polysaccharide into a solution, deproteinizing by a Sevag method, depigmenting by an H 2O2 method, dialyzing by a dialysis bag (3500 kDa), and freeze-drying to obtain black corn polysaccharide, and preserving for later use. Example 2 experiments on Black corn polysaccharide to i