CN-117050487-B - Light-responsive ordered porous membrane material for spontaneous cell adhesion and desorption and preparation method thereof

Abstract

The invention discloses a light-responsive ordered porous membrane material for spontaneous cell adhesion and desorption and a preparation method thereof. The porous membrane material component comprises a polymer and a light response compound spiropyran, wherein the polymer is at least one of polylactic acid and polystyrene. The preparation method of the porous membrane material comprises (1) dissolving the polymer and the light-responsive compound in the solvent, (2) dripping the prepared solution onto the surface of the substrate, placing the substrate on a rotating platform of a spin coater, (3) introducing air into the spin coater to blow air flow from the right upper side of the solution to the solution, controlling the speed of the introduced air, the temperature and relative humidity in a chamber of the spin coater, and simultaneously starting the spin coater and controlling the rotating speed of the spin coater, (4) stopping the spin coater after the solvent is completely volatilized, and taking out the substrate to obtain the ordered porous membrane material. The ordered porous membrane can enable cells to adhere spontaneously and desorb spontaneously, so that the success rate and the yield of cell culture are improved.

Inventors

• YIN HONGYAO
• LI ZONGCHENG
• FENG YUJUN

Assignees

• 四川大学

Dates

Publication Date

20260505

Application Date

20221129

Claims (5)

1. 1. A light-responsive ordered porous membrane material for spontaneous cell adhesion and desorption is characterized by comprising a polymer and a light-responsive compound, wherein the light-responsive compound is spiropyran, and the spiropyran has the structural formula of , The polymer is at least one of polylactic acid and polystyrene; The mass ratio of the spiropyran to the polymer is 1:4; the preparation method comprises the following steps: (1) Dissolving a polymer and a light-responsive compound in a solvent to prepare a uniform solution; (2) Dripping the prepared solution onto the surface of a substrate, and placing the substrate on a rotating platform of a spin coater; (3) Introducing air into the spin coater to enable air flow to blow the solution from the right above, controlling the speed of the introduced air, the temperature and the relative humidity in the spin coater chamber, and simultaneously starting the spin coater and controlling the rotating speed of the spin coater; (4) Stopping the spin of the spin coater after the solvent is completely volatilized, and taking out the substrate to obtain the ordered porous membrane material.
2. 2. The light-responsive ordered porous membrane material of claim 1, wherein the solvent is at least one of dichloromethane, chloroform, carbon disulfide.
3. 3. The light-responsive ordered porous film material of claim 1, wherein the substrate is glass, silicon wafer or polyester film.
4. 4. The ordered porous membrane material with light response according to claim 1, wherein the concentration of the polymer in the solution obtained in the step (1) is 5-50 mg/mL, the concentration of the light response compound is 0.01-2 mg/mL, the concentration of the polymer in the solution obtained is 5-30 mg/mL, and the concentration of the light response compound is 0.01-1.5 mg/mL.
5. 5. The light-responsive ordered porous membrane material according to claim 1, wherein the air velocity introduced in the step (3) is 100-300L/h, the temperature in a spin coater chamber is 20-28 o C, the relative humidity in the spin coater chamber is 50% -90%, and the rotating speed of the spin coater is 0-50 rpm.

Description

Light-responsive ordered porous membrane material for spontaneous cell adhesion and desorption and preparation method thereof Technical Field The invention belongs to the field of polymer porous materials and cell culture, and particularly relates to a light-responsive ordered porous membrane material. Background The surface ordered pore structure of the porous membrane provides a good substrate for cell adhesion, and the adhesion of cells on the surface of the porous membrane has selectivity. The stimulus response functional group is introduced on the surface of the membrane, so that the intelligent porous membrane material with reversible stimulus response function in surface property is constructed, hydrophilic secondary treatment after membrane formation can be avoided, and the regulation and control of the adhesion and desorption behaviors of cells on the surface of the membrane can be realized only by changing external stimulus. However, in cell culture, redundant operation and easy pollution of cells are caused, other variables are introduced to cause adverse effects on cell culture, and meanwhile, environmental stimulus can cause damage to cells. Therefore, an ordered porous membrane which can enable cells to adhere and desorb spontaneously is designed, and the success rate and the yield of cell culture can be greatly improved. Disclosure of Invention Aiming at the defects of the prior art, the invention provides a light-responsive ordered porous membrane material for self-adhesion and desorption of cells and a preparation method thereof, so as to obtain an ordered porous membrane with macroscopic color and surface wettability and light responsiveness, which enables the cells to adhere and desorb spontaneously, thereby improving the success rate and the yield of cell culture. Relative to the pH and temperature stimulus, the light stimulus belongs to non-contact response, and has the characteristics of easy acquisition, strong remote control, accurate and rapid response regulation and control, and the like. Based on these advantages of light stimulation, it is expected to solve the above problems to introduce specific photo-sensitive elements on the surface of the porous film to change the response to the light and to give the porous film specific light control surface wettability. Spiropyrans are a typical class of photochromic photo-motifs, in which two aromatic heterocycles of the class of compounds are linked by an SP3 hybridized spirocarbon atom, the two ring systems are mutually orthogonal without conjugation, and the molecular structure is in a closed-loop State (SP). When irradiated by light of a certain wavelength, chemical bonds C-O-C in the molecule are broken, two ring systems which are mutually orthogonal are changed into a plane structure, the whole molecule forms a conjugated system, and the molecular structure is changed into an open-loop structure (MC). The change of the molecular structure causes the absorption spectrum to change obviously, and the macroscopic color change is obvious. When the spiropyran is in a dark condition, the spiropyran is automatically changed from an open-loop state to a closed-loop state, and the original color is recovered. The transition between the two states of the spiropyran, in addition to leading to a reversible change in its macroscopic color, also leads to a reversible change in its hydrophilic/hydrophobic properties. The closed-loop state is hydrophobic, and the open-loop state is ionic, thus exhibiting good hydrophilicity. It is expected that if a reasonable spiropyran molecule is designed and introduced to the surface of an ordered porous membrane, it is expected to construct a light-responsive intelligent ordered porous membrane. In the hydrophilic MC state, cells are adhered to the porous membrane, and after that, the culture is continued, the MC state spontaneously converts to the hydrophobic SP state, and thus the cells are spontaneously desorbed. The invention provides a light-responsive ordered porous membrane material for spontaneous cell adhesion and desorption, which comprises a polymer and a light-responsive compound, wherein the light-responsive compound is spiropyran, and the spiropyran has the structural formula of 。 Further, the polymer is at least one of polylactic acid and polystyrene, preferably polystyrene. Further, the mass ratio of the spiropyran to the polymer in the porous membrane material is preferably 1:4. The preparation method of the light-responsive ordered porous membrane material provided by the invention comprises the following steps: (1) Dissolving a polymer and a light-responsive compound in a solvent to prepare a uniform solution; (2) Dripping the prepared solution onto the surface of a substrate, and placing the substrate on a rotating platform of a spin coater; (3) Introducing air into the spin coater to enable air flow to blow the solution from the right above, controlling the speed of the introduced air, the