CN-117126787-B - Lactobacillus fermentum and application of fermentation product thereof in prevention and control of mycotoxin pollution of agricultural products

Abstract

The invention provides lactobacillus fermentum and application of a fermentation product thereof in prevention and control of mycotoxin pollution of agricultural products, and relates to the technical field of food safety prevention and control. The research on lactobacillus fermentum with the preservation number of CGMCC 1.15608 shows that the strain and the fermentation product thereof have good prevention and control effects on mycotoxins, can effectively inhibit the formation of mycotoxin pollution in crops, and can be used for preparing mycotoxin inhibitors.

Inventors

• Zhan Chengfang
• WANG MENGCEN
• Matsumoto motoharu
• LIU XIAOYU
• LV TIANXING
• FANG HONGDA
• PAN QIANQIAN
• XU HAORONG
• CHENG RUI

Assignees

• 浙江大学

Dates

Publication Date

20260512

Application Date

20231017

Claims (9)

1. 1. The application of the lactobacillus fermentum and the fermentation product thereof in prevention and control of mycotoxin pollution of agricultural products is characterized in that the lactobacillus fermentum is preserved in China general microbiological culture collection center with the preservation number of CGMCC 1.15608; the mycotoxin is UA toxin.
2. 2. The use according to claim 1, wherein the agricultural product comprises rice, wheat or corn.
3. 3. The use according to claim 1, wherein the fermentation product is prepared by fermenting the lactobacillus fermentum to obtain a fermentation broth, and then separating the fermentation broth to obtain the fermentation product.
4. 4. The application of the lactobacillus fermentum and the fermentation product thereof in preparing the mycotoxin inhibitor is characterized in that the lactobacillus fermentum is preserved in China general microbiological culture collection center with the preservation number of CGMCC 1.15608; the mycotoxin is UA toxin.
5. 5. The use according to claim 4, wherein the mycotoxin inhibitor further comprises an adjuvant; The auxiliary agent comprises at least one of a solvent, a cosolvent, an emulsifier, a wetting agent, a dispersing agent, an adhesive and a stabilizer.
6. 6. The use according to claim 4, wherein the mycotoxin inhibitor is in a dosage form comprising a powder, granule, suspension, oil, fumigant, sustained release formulation, seed coating formulation, microcapsule formulation, effervescent tablet or spray formulation.
7. 7. A method for preventing and controlling mycotoxin pollution is characterized by comprising the steps of soaking seeds of crops with lactobacillus fermentum, a fermentation product of lactobacillus fermentum or a mycotoxin inhibitor as set forth in any one of claims 3-6; The lactobacillus fermentum is preserved in China general microbiological culture collection center with a preservation number of CGMCC 1.15608; the mycotoxin is UA toxin.
8. 8. The control method according to claim 7, wherein the time of the seed soaking treatment is 10 hours or more; The seeds are seeds with germination accelerating completion.
9. 9. The method of claim 7, further comprising spraying lactobacillus fermentum, a fermentation product of lactobacillus fermentum, or a mycotoxin inhibitor of any one of claims 3-6 during the heading stage.

Description

Lactobacillus fermentum and application of fermentation product thereof in prevention and control of mycotoxin pollution of agricultural products Technical Field The invention relates to the technical field of food safety prevention and control, in particular to lactobacillus fermentum and application of a fermentation product thereof in prevention and control of mycotoxin pollution of agricultural products. Background Agricultural product biotoxin contamination generally involves mycotoxin contamination of the edible parts of the crop, which poses a serious threat to food safety and human health. Mycotoxins are often produced during the growth and storage of crops, especially under humid and warm conditions. Different types of mycotoxins can affect a variety of food crops including rice, wheat, corn, and the like. Taking rice as an example, false smut, etc. are a destructive fungus disease seriously harming rice production, and the pathogen is ustilaginoidea virens, also called Rhizoctonia cerealis (Ustilaginoidea virens). It is widely distributed in more than 40 countries in asia, africa, south america, europe, etc. in major rice producing regions. With the change of the cultivation system of rice, the large amount or unreasonable use of chemical fertilizers and the like, the false smut hazard is aggravated year by year, and the secondary diseases of rice are raised to be main diseases. The specific interaction between virens and glume flowers is a model fungus of flower infection pathogenic fungi, which is parasitic on flower organs through hyphae, and finally forms rice false smut balls on the scions. The formation of the rice false smut not only affects the growth and development of seeds and the grouting process, but also causes serious yield and economic loss. The main mycotoxin produced by ustilaginoidea virens is Ustiloxins A (UA), which can prevent the polymerization of microtubulin in mammalian cells, interfere the formation of cytoskeleton, inhibit cell mitosis, and cause toxic phenomena such as viscera lesions and the like in agricultural products polluted by toxin ingestion after misingestion, thus having higher diet ingestion risk. In addition, rice, wheat, corn are also often infested with the fungus Fusarium, which is capable of producing Fumonisins (FB) after a spike infestation, the most common of which is FB1 toxin, which poses a hazard to human and animal health. Short term exposure may lead to toxic symptoms such as nausea, vomiting, diarrhea, headache, eye stinging, etc. Prolonged or high dose exposure may cause more serious health problems. At present, mycotoxin pollution in grain crops has no relevant prevention and control strategy. In agricultural production, in order to prevent mycotoxin pollution, diseases are often prevented by applying chemical agents, but not only the problem of pesticide residues is easily caused, but also the problem of combined pollution of mycotoxins and bactericides is caused. In view of this, the present invention has been made. Disclosure of Invention A first object of the present invention is to provide use of lactobacillus fermentum (Lactobacillus fermentum, lf) and its fermentation products in the prevention and control of mycotoxin contamination of agricultural products to solve at least one of the above problems. A second object of the present invention is to provide the use of lactobacillus fermentum and its fermentation products for the preparation of mycotoxin inhibitors. The third object of the invention is to provide a method for preventing and controlling mycotoxin pollution. In a first aspect, the invention provides application of lactobacillus fermentum and a fermentation product thereof in prevention and control of mycotoxin pollution of agricultural products, wherein the lactobacillus fermentum is preserved in China general microbiological culture collection center with a preservation number of CGMCC 1.15608. As a further embodiment, the mycotoxin comprises a UA toxin or FB toxin. As a further aspect, the agricultural product comprises rice, wheat or corn. The preparation method of the fermentation product comprises the steps of fermenting the lactobacillus fermentum to obtain fermentation liquor, and separating the fermentation liquor to obtain the fermentation product. In a second aspect, the invention provides the application of lactobacillus fermentum and a fermentation product thereof in preparing a mycotoxin inhibitor, wherein the lactobacillus fermentum is preserved in China general microbiological culture collection center with a preservation number of CGMCC 1.15608. As a further technical scheme, the mycotoxin inhibitor further comprises an auxiliary agent; The auxiliary agent comprises at least one of a solvent, a cosolvent, an emulsifier, a wetting agent, a dispersing agent, an adhesive and a stabilizer. As a further technical scheme, the dosage form of the mycotoxin inhibitor comprises powder, granules, suspending agents, oil