CN-117147828-B - Diluent for drug fluorescence detection and preparation method and application thereof

Abstract

The invention discloses a diluent for drug fluorescence detection, a preparation method and application thereof. The diluent comprises a buffering agent, a stabilizing agent, inorganic salt and a dispersing agent, wherein the buffering agent comprises any one or a combination of at least two of tris (hydroxymethyl) aminomethane, phosphate or carbonate, the stabilizing agent comprises any one or a combination of at least two of bovine serum albumin, casein or PEG, and the dispersing agent comprises any one or a combination of at least two of polyvinyl alcohol, polyethylene glycol or lauryl alcohol. The invention creatively discovers that the diluent composed of the buffer, the stabilizer, the inorganic salt and the dispersing agent can well promote the specificity and the uniformity of drug detection reagent products, increase the stability of the products, reduce the difference between the products, improve the detection accuracy, be applicable to the rapid screening in public places, and in addition, the components are safer and do not cause environmental pollution.

Inventors

• FANG SHAOHUA
• YU HONGJIANG
• KONG QINGBO
• HUANG WEIZHONG
• LI ZHANFEI
• MA RONG

Assignees

• 浙江东方基因生物制品股份有限公司

Dates

Publication Date

20260505

Application Date

20230907

Claims (10)

1. 1. The diluent for drug fluorescence detection is characterized by comprising, by mass, 100% of a buffer, 0.5% -3.5% of a stabilizer, 0.1% -0.3% of an inorganic salt, 0.4% -0.8% of a dispersing agent, 0.5% -10% of a preservative and the balance of water; The buffer is tris (hydroxymethyl) aminomethane; The stabilizer is a combination of bovine serum albumin and casein; the dispersing agent is polyvinyl alcohol.
2. 2. The diluent for fluorescence detection of drugs of claim 1, wherein the inorganic salt comprises any one or a combination of at least two of sodium chloride, potassium chloride or borate.
3. 3. The diluent for fluorescence detection of drugs of claim 1, wherein the preservative comprises any one or a combination of at least two of Proclin300, sodium azide.
4. 4. A method for preparing a diluent for fluorescence detection of drugs according to any one of claims 1-3, characterized in that the method comprises: and mixing the buffering agent, the stabilizing agent, the inorganic salt, the dispersing agent, the preservative and water to obtain the diluent.
5. 5. Use of a diluent for fluorescence detection of drugs according to any of claims 1-3 for the preparation of a product for detecting drugs.
6. 6. A method of preparing a drug fluorescence detection test strip, the method comprising: diluting by mixing the diluent for drug fluorescence detection according to any one of claims 1-3 with the antibody marked by the fluorescent marker and coating the mixture on a binding pad; and sequentially adhering the sample pad, the binding pad, the detection membrane and the absorption pad on the surface of the substrate from one end to the other end of the substrate to obtain the drug fluorescence detection test strip.
7. 7. The method of claim 6, wherein the fluorescent label comprises a fluorescent microsphere or a magnetic bead microsphere.
8. 8. The method for preparing a drug fluorescence detection test strip according to claim 6, wherein the dilution factor of the dilution is 5-20 times.
9. 9. The method of preparing a drug fluorescence detection test strip of claim 6, wherein the drug comprises any one or a combination of at least two of morphine, anilides, ketamine, tetrahydrocannabinic acid, cannabinoids, or cocaine.
10. 10. Use of a diluent for fluorescence detection of drugs according to any one of claims 1-3 and a method for preparing a fluorescence detection test strip for drugs according to any one of claims 6-9 in drug detection.

Description

Diluent for drug fluorescence detection and preparation method and application thereof Technical Field The invention belongs to the technical field of drug detection, relates to a diluent for drug fluorescence detection and a preparation method and application thereof, and in particular relates to a diluent for hair poisoning fluorescence detection and a preparation method and application thereof Background The traditional quick detection technology for drug-taking personnel mainly adopts a urine colloidal gold method for only about 24-48 hours. Compared with biological detection materials such as urine, blood, saliva and the like, the hair detection material has the advantages of stable property, convenient material taking, easy storage, long detection time limit, wide application range, less pollution opportunity and the like. Because the content of urine poisoning products and metabolites thereof is very high, the traditional colloidal gold immunoassay technology can be achieved. However, hair intoxicants and their metabolites are very low in content, and therefore, techniques with higher detection sensitivity and better tamper resistance are required for hair detection. At present, a drug detection test strip usually adopts fluorescent microspheres to mark antibodies or antigens, the fluorescent microspheres are functional microspheres, fluorescent substances are marked on the surfaces of the microspheres through chemical reaction or physical adsorption, or certain fluorescent substances are embedded or polymerized in the microspheres, so that the microspheres have fluorescent display effect, and the appearance of the fluorescent microspheres can be any shape, mainly spherical. Currently, the types of fluorescent microspheres include quantum dot fluorescent microspheres, rare earth fluorescent microspheres, self-luminescent fluorescent microspheres, and the like. The fluorescent microsphere has the advantages of uniform granularity, good dispersibility, high luminous efficiency and the like, but has poor stability and can be influenced by external environment and medium. Photobleaching or photolysis may occur over time or under intense light, resulting in signal decay of the fluorescent microspheres. When a plurality of different fluorescent dyes are used to label the fluorescent microspheres, the different fluorescent signals may interfere or overlap with each other, affecting interpretation and quantitative analysis of the results. In the existing preparation process of fluorescent drug detection reagent, buffer solution or inorganic salt solution is often used for diluting and preparing fluorescent substance-marked antibody or antigen, and coating is carried out, so that low detection sensitivity, poor specificity and batch-to-batch instability are easy to cause false negative results and false positive results, for example, CN110850095A discloses a drug trace detection test strip, a preparation method and a kit thereof, and a time-resolved fluorescent microsphere-marked specific monoclonal antibody is uniformly dispersed in phosphate buffer solution. In summary, the development of the novel diluent is used for re-dissolving and diluting after the fluorescent label is finished in the preparation process of the detection test strip, so that the stability and uniformity of fluorescent signals are improved, and the novel diluent has important significance in the field of drug fluorescence detection. Disclosure of Invention Aiming at the defects and actual demands of the prior art, a diluent for drug fluorescence detection and a preparation method and application thereof, in particular to a diluent for hair poisoning fluorescence detection and a preparation method and application thereof, so as to improve detection stability and accuracy. In order to achieve the above purpose, the invention adopts the following technical scheme: In a first aspect, the invention provides a diluent for drug fluorescence detection, which comprises a buffer, a stabilizer, inorganic salts and a dispersing agent, wherein the buffer comprises any one or a combination of at least two of tris (hydroxymethyl) aminomethane, phosphate and carbonate, the stabilizer comprises any one or a combination of at least two of bovine serum albumin, casein and PEG, and the dispersing agent comprises any one or a combination of at least two of polyvinyl alcohol, polyethylene glycol and lauryl alcohol. In the invention, a specific diluent formula is designed, and the diluent formula comprises a buffering agent, a stabilizing agent, inorganic salt and a dispersing agent, wherein the buffering agent and the inorganic salt are used for diluting an antibody or an antigen marked by a fluorescent marker in the coating process of a test paper binding pad (or a fluorescent pad), the buffering agent and the inorganic salt provide a proper ionic environment for the fluorescent marker by adjusting the ionic concentration of a system, the stabilizing agent