CN-117180514-B - Simple preparation and application of ECM paper bracket

Abstract

The invention belongs to the technical field of medical materials, and discloses simple preparation and application of an ECM paper bracket. Specifically disclosed is an ECM paper scaffold prepared from materials including decellularized ECM and hexafluoroisopropanol. The ECM paper scaffold provided by the invention has concave folds with different depths, and meanwhile, obvious penetrating concave holes can be formed, so that the ECM paper scaffold has good swelling rate, hydrophilicity and permeability and high tissue state stability. Cells (such as HUVECs) can be adhered to the ECM paper bracket rapidly, obvious tentacles can be adhered to the ECM paper bracket, the cells can be well extended and spread, proliferation of the cells is promoted, functional expression of the cells (such as secretion of HUVECs NO) is effectively promoted, deep internal exudation of nutrients and oxygen is effectively promoted, and cell growth is promoted.

Inventors

• XIAO JIANGWEI
• GAO BOTAO
• WU HENGPENG

Assignees

• 广东省科学院生物与医学工程研究所

Dates

Publication Date

20260505

Application Date

20230829

Claims (7)

1. 1. The ECM paper bracket is prepared by mixing decellularized ECM with hexafluoroisopropanol, curing and forming, wherein the ECM paper bracket is provided with concave folds and penetrating concave holes with different depths, the mass volume ratio of the decellularized ECM to the hexafluoroisopropanol is (8-15) mg/1 mL, and the mixing time is 24-30 h.
2. 2. The ECM paper scaffold of claim 1, wherein the decellularized ECM comprises a cell-derived decellularized ECM or a tissue-derived decellularized ECM.
3. 3. The ECM paper scaffold according to any one of claims 1 to 2, wherein the decellularized ECM is prepared by digesting and re-suspending cells, culturing the cells in a wall-attached manner, and performing differentiation culture on the cells in a differentiation medium to obtain primitive endoderm-like cells, and performing decellularization treatment on the primitive endoderm-like cells with ammonia water.
4. 4. The ECM paper scaffold of any one of claims 1-3 for use in any one of (1) - (4): (1) Preparing a tissue substitute; (2) Screening medicines; (3) Case model research; (4) An in vitro 3D tissue model was constructed.
5. 5. The ECM paper scaffold of any one of claims 1-3 for use in any one of (5) - (8): (5) Culturing cells; (6) Preparing a product of the cultured cells; (7) Promoting cellular functional expression for non-therapeutic purposes; (8) Preparing a product for promoting the expression of cellular functions.
6. 6. Use of an ECM paper scaffold according to any one of claims 1 to 3 for the preparation of a tissue repair product.
7. 7. A product comprising the ECM paper scaffold of any one of claims 1-3.

Description

Simple preparation and application of ECM paper bracket Technical Field The invention belongs to the technical field of medical materials, and particularly relates to simple preparation and application of an ECM paper bracket. Background Decellularized ECM (extracellular matrix ) is widely used in tissue engineering as a biomaterial for promoting cell migration, proliferation, differentiation and expression of functions due to its physiological functions and microstructure and the inclusion of bioactive substances such as proteins and growth factors that promote cell growth. ECM biomaterials currently used are mainly decellularized ECM derived from animal tissues, organs, and cell culture derived decellularized ECM. However, in the application research, the application of the animal tissue or organ derived decellularized ECM in tissue engineering is greatly limited due to factors such as batch-to-batch difference, complex composition, instability, strong immunogenicity, risk of virus carrying, source limitation and the like. In the culture process, the cell-derived decellularized ECM can realize batch and component-stable production of the decellularized ECM through in-vitro large-scale and regulatory culture, and can realize smaller immunogenicity and avoid the risk of virus carrying. Stem cell therapy is an effective treatment in regenerative medicine, and is a potential treatment regimen for a wide range of injuries and degenerative diseases. The current research shows that the stem cell treatment has wide application prospect in clinic. However, the therapeutic effect is still limited because the current stem cell injection treatment cannot collect the implanted cells at a specific implantation site for a long time, and an implantation method requiring a large amount of cells can achieve the therapeutic effect. Therefore, based on the limitations, biomaterials are applied as cell delivery vehicles in stem cell therapy, and the biomaterials promote cell attachment and proliferation, then deliver and concentrate cells to implantation sites for a long time, and promote migration, proliferation, differentiation, functional expression, and the like of implanted cells. The biological material used as the cell delivery carrier has excellent biocompatibility, mechanical property, lower immunogenicity, maintenance and promotion of cell migration and functional expression, etc. Based on the excellent cell compatibility, low immunogenicity and controllable mechanical property of the decellularized ECM, the decellularized ECM can be used as an ideal biological material of a cell delivery carrier to promote cell implantation and migration, proliferation, differentiation and functional expression. However, the use of biological materials as cell delivery vehicles has currently faced some difficulties. For example, cells cultured on biological materials grow prior to the surface of the material and gradually migrate into the interior of the material, requiring a longer construction period for the desired biological material-cell body construction, and thus are disadvantageous for their use. The single matrix constructed by the biological material can not simulate the physiological structure of multiple layers of biological tissues, so that the construction of an ideal biological material-cell body needs to design materials with proper size and reasonable microstructure and porosity, the survival and the growth of the cells in the material can be effectively promoted by the oxygen and the nutrient substances penetrating the material, the construction of three-dimensional tissues with bionic multi-layer structures is facilitated, and the repair treatment of different damaged tissue positions and the implantation of the cells to promote the tissue damage is achieved. Disclosure of Invention The present invention aims to solve at least one of the above-mentioned problems and problems in the prior art. It is an object of a first aspect of the present invention to provide an ECM paper scaffold. The object of the second aspect of the present invention is to provide a method for preparing an ECM paper scaffold according to the first aspect of the present invention. The object of a third aspect of the present invention is to provide the use of an ECM paper scaffold of the first aspect of the invention and/or the method of preparation of the second aspect of the invention for the preparation of a tissue substitute. The object of the fourth aspect of the present invention is to provide the use of the ECM paper scaffold of the first aspect of the invention and/or the method of preparation of the second aspect of the invention in cell culture. The object of a fifth aspect of the present invention is to provide the use of an ECM paper scaffold of the first aspect of the invention and/or the method of preparation of the second aspect of the invention for the preparation of a tissue repair product. The object of the sixth aspect of t