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CN-117230548-B - Modified wheat bran fiber and preparation and application thereof

CN117230548BCN 117230548 BCN117230548 BCN 117230548BCN-117230548-B

Abstract

The invention belongs to the field of comprehensive utilization of agricultural product byproducts, and particularly relates to modified wheat bran fibers, and preparation and application thereof. In the invention, the water-soluble components and the soluble dietary fibers in the wheat bran are removed by adopting an ultrahigh pressure treatment mode under the ultrahigh pressure condition, which is favorable for extracting insoluble dietary fibers, and the adsorption capacity of the wheat bran is greatly improved by the synergistic treatment of sodium hydroxide, epichlorohydrin and dimethylamine after the wheat bran is subjected to the ultrahigh pressure treatment, and the synergistic effect is achieved by physical modification and chemical modification.

Inventors

  • HUANG JIHONG
  • WANG JUAN
  • QUAN WENBIN

Assignees

  • 许昌学院
  • 华南理工大学

Dates

Publication Date
20260505
Application Date
20230911

Claims (8)

  1. 1. The preparation method of the modified wheat bran fiber is characterized by comprising the following steps of: 1) Pulverizing testa Tritici, and sieving to obtain testa Tritici powder; 2) Adding water into the wheat bran powder, and performing ultrahigh pressure treatment to obtain a wheat bran powder mixed solution; 3) Centrifugally separating the wheat bran powder mixed solution obtained in the previous step, discarding supernatant, adding water into the collected precipitate, stirring, standing, centrifugally separating again, and collecting the precipitate; 4) Adding water into the precipitate obtained in the previous step, adding amylase, adjusting the pH value to 3.5-4.0, performing heat-preserving vibration reaction, adding saccharifying enzyme, performing heat-preserving vibration reaction, performing centrifugal separation, and collecting the precipitate; 5) Adding water and protease into the precipitate obtained in the previous step, adjusting the pH value to 7.0-7.5, carrying out heat preservation and oscillation reaction, centrifuging, and collecting the precipitate to obtain insoluble wheat bran fiber; 6) Adding NaOH solution into insoluble fiber of wheat bran obtained in the previous step, stirring, standing, centrifuging, and collecting precipitate; 7) Adding epichlorohydrin solution into the precipitate obtained in the previous step, stirring, filtering, and washing filter residues with water and acetone in sequence; 8) Adding dimethylamine solution into the filter residue obtained in the previous step, stirring for reaction, filtering, and washing the filter residue with water and acetone in sequence; 9) And (3) drying the filter residues obtained in the previous step to obtain the modified wheat bran fiber.
  2. 2. The preparation method of claim 1, wherein the mass of water in the step 2) is 2-3 times of the mass of wheat bran powder, and the condition of the ultrahigh pressure treatment is room temperature and 200-400MPa for 10-15 minutes.
  3. 3. The method of claim 1, wherein in the step 6), the concentration of the NaOH solution is 20-30%, and the addition amount is 10-15 times of the mass of insoluble fiber of wheat bran.
  4. 4. The method of claim 1, wherein in the step 7), the concentration of the epichlorohydrin solution is 99.0 to 99.5%, the addition amount is 4 to 8 times of the mass of the precipitate, the stirring time is 4 to 8 hours, and the mass of the water and the acetone is 2 to 3 times of the mass of the filter residue.
  5. 5. The preparation method of the catalyst according to claim 1, wherein in the step 8), the concentration of the dimethylamine solution is 30% -35%, the addition amount is 4-8 times of the mass of the filter residue, the reaction is carried out at 60-80 ℃ for 3-5 hours, the water and the acetone are 2-3 times of the mass of the filter residue, and in the step 9), the drying condition is 80-100 ℃ and 10-14 hours.
  6. 6. The process according to claim 1, wherein the conditions for the centrifugation are 2000-3000 rpm, centrifugation for 10-15 minutes, and a reaction at 50-55℃for 2-3 hours with shaking.
  7. 7. The modified wheat bran fiber produced by the method of claim 1.
  8. 8. Use of the modified wheat bran fiber of claim 7 in a dye adsorbent.

Description

Modified wheat bran fiber and preparation and application thereof Technical Field The invention belongs to the field of comprehensive utilization of agricultural product byproducts, and particularly relates to modified wheat bran fibers, and preparation and application thereof. Background Wheat bran is the outer layer part of wheat grains, and the grains are obtained by grinding and separating in the wheat milling process, and the wheat bran is about one fourth of the mass of the original wheat grains and is an important byproduct in the wheat processing process. The wheat bran has rich yield, is mainly used in the fields of feed, fermentation and the like at present, is not sufficiently processed and utilized, has application value to be further explored, and therefore, the development and utilization of the wheat bran are enhanced, the resource waste can be effectively reduced, and the added value of the wheat industry is increased. Disclosure of Invention In view of the above, the present invention aims to provide a modified wheat bran fiber, a preparation method of the wheat bran fiber, and an application of the wheat bran fiber in adsorption. The technical scheme adopted by the invention is as follows: The preparation method of the modified wheat bran fiber is characterized by comprising the following steps of: 1) Pulverizing testa Tritici, and sieving to obtain testa Tritici powder; 2) Adding water into the wheat bran powder, and performing ultrahigh pressure treatment to obtain a wheat bran powder mixed solution; 3) Centrifugally separating the wheat bran powder mixed solution obtained in the previous step, discarding supernatant, adding water (the water dosage is 2-3 times of the sediment mass) into the collected sediment, stirring, standing (1-1.5 h), centrifugally separating again, and collecting the sediment; 4) Adding water into the precipitate obtained in the previous step, adding amylase, adjusting the pH value to 3.5-4.0, performing heat-preserving vibration reaction, adding saccharifying enzyme, performing heat-preserving vibration reaction, performing centrifugal separation, and collecting the precipitate; 5) Adding water and protease into the precipitate obtained in the previous step, adjusting the pH value to 7.0-7.5, carrying out heat preservation and oscillation reaction, centrifuging, and collecting the precipitate to obtain insoluble wheat bran fiber; 6) Adding NaOH solution into insoluble fiber of wheat bran obtained in the previous step, stirring, standing (1-1.5 h), centrifuging, and collecting precipitate; 7) Adding epichlorohydrin solution into the precipitate obtained in the previous step, stirring, filtering, and washing filter residues with water and acetone in sequence; 8) Adding dimethylamine solution into the filter residue obtained in the previous step, stirring for reaction, filtering, and washing the filter residue with water and acetone in sequence; 9) And (3) drying the filter residues obtained in the previous step to obtain the modified wheat bran fiber. The mass of water in the step 2) is 2-3 times of that of wheat bran powder, and the condition of ultrahigh pressure treatment is room temperature, 200-400MPa and 10-15 minutes. In the step 4), the water consumption is 1-1.5 times of the precipitation mass, the amylase consumption is 100-150U/100g of mixed liquor, and the saccharifying enzyme consumption is 60-100U/100g of mixed liquor. In the step 5), the water consumption is 1-1.5 times of the precipitation mass, and the protease consumption is 150-200U/100g of the mixed solution. In the step 6), the concentration of the NaOH solution is 20-30%, and the adding amount is 10-15 times of the mass of insoluble fibers of the wheat bran. In the step 7), the concentration of the epichlorohydrin solution is 99.0-99.5%, the adding amount is 4-8 times of the mass of the sediment, the stirring time is 4-8h, and the mass of the water and the acetone is 2-3 times of the mass of the filter residue. In the step 8), the concentration of dimethylamine solution is 30% -35%, the adding amount is 4-8 times of the mass of filter residues, the reaction is carried out for 3-5 hours at 60-80 ℃ during the reaction, the water and the acetone are 2-3 times of the mass of the filter residues, and in the step 9), the drying condition is 80-100 ℃ and 10-14 hours. The centrifugal separation condition is 2000-3000 rpm, centrifugal for 10-15 min, and heat insulating vibration reaction at 50-55 deg.c for 2-3 hr. The modified wheat bran fiber prepared by the method is provided. The application of the modified wheat bran fiber in the aspect of dye adsorbent. The inventor researches on the comprehensive utilization of wheat bran for many years, and is always explored in the application of wheat bran fibers. The inventor also found that dye wastewater is one of common water pollution, the main components of the dye wastewater comprise aromatic rings, amino groups and azo groups, the components are comple