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CN-117264092-B - Nanometer antibacterial material for reducing Fe (III) by gamma-cyclodextrin and preparation method and application thereof

CN117264092BCN 117264092 BCN117264092 BCN 117264092BCN-117264092-B

Abstract

The invention discloses a preparation method of a food-grade Fe-gamma-CD nano antibacterial material, which belongs to the technical field of food preservation and comprises the specific steps of dissolving FeCl 3 ·6H 2 O and gamma-CD in deionized water for ultrasonic treatment, heating the mixture for 5-7 hours at 120-180 ℃ with the molar ratio of 1:2-1:3 of the gamma-CD to the FeCl 3 ·6H 2 O, centrifuging after reaction, removing supernatant, collecting precipitate, continuously washing the precipitate with deionized water, repeatedly centrifuging, removing impurities, placing a centrifuged precipitate sample in a blast drying oven, and drying the sample thoroughly to finish the preparation of the Fe-gamma-CD nano antibacterial material. The Fe-gamma-CD nano antibacterial material has the advantages of excellent peroxide catalytic activity, broad-spectrum antibacterial effect, high tolerance to severe environment, high stability and the like, and the Fe-gamma-CD is non-toxic to normal cells, and belongs to food-grade antibacterial materials.

Inventors

  • LIU CHENGZHEN
  • LIU XIAOHUI
  • XU YUANHONG
  • ZHAO YUHAN
  • PAN WEIQIANG

Assignees

  • 青岛大学

Dates

Publication Date
20260512
Application Date
20230922

Claims (8)

  1. 1. The preparation method of the food-grade Fe-gamma-CD nano antibacterial material is characterized by comprising the following specific steps of: (1) Dissolving FeCl 3 •6H 2 O and gamma-CD in deionized water for ultrasonic treatment, wherein the molar ratio of the gamma-CD to the FeCl 3 •6H 2 O is 1:2-1:3; (2) Heating at 120deg.C for 5-7 hr; (3) Centrifuging after the reaction, removing supernatant, collecting precipitate, continuously washing the precipitate with deionized water, repeatedly centrifuging, removing impurities, placing the centrifuged precipitate sample in a forced air drying oven, and drying to finish the preparation of the Fe-gamma-CD nano antibacterial material.
  2. 2. The method for preparing a food-grade Fe- γ -CD nanostatic material according to claim 1, wherein the heating time in step (2) is 6 hours.
  3. 3. The method for preparing a food-grade Fe-gamma-CD nano antibacterial material according to claim 1, wherein the step (3) is that the reacted sample is taken out, centrifuged for 10 minutes at 8000 rpm, the supernatant is removed, the precipitate is collected, the precipitate is further washed with deionized water, the centrifugation is repeated for 3 times, the centrifuged precipitate sample is placed in a blast drying oven at 60 ℃, and the sample is dried thoroughly, thus the preparation of the Fe-gamma-CD nano antibacterial material is completed.
  4. 4. The method for preparing a food-grade Fe- γ -CD nanomaterial according to claim 1, wherein the Fe- γ -CD nanomaterial has a rod-like structure with a length of 150 nm-200 nm and a width of 50 nm.
  5. 5. The method for preparing a food-grade Fe- γ -CD nano-antibacterial material according to claim 1, wherein the molar ratio of γ -CD to FeCl 3 •6H 2 O is 1:3, the heating time is 6 hours, and the heating temperature is 120 ℃, and the prepared Fe- γ -CD nano-antibacterial material contains 6/11 of Fe 2+ and 5/11 of Fe 3+ .
  6. 6. The Fe- γ -CD nanosized antibacterial material prepared by the method for preparing a food-grade Fe- γ -CD nanosized antibacterial material according to any one of claims 1 to 5.
  7. 7. The use of the Fe- γ -CD nanoantibacterial material according to claim 6 as a non-therapeutic antibacterial material.
  8. 8. The use of the Fe-gamma-CD nano-antibacterial material according to claim 6 for preserving milk and orange juice.

Description

Nanometer antibacterial material for reducing Fe (III) by gamma-cyclodextrin and preparation method and application thereof Technical field: The invention belongs to the technical field of food preservation, and particularly relates to a gamma-cyclodextrin reduced Fe (III) nano antibacterial material, and a preparation method and application thereof. The Fe-gamma-CD nano material is prepared by a one-pot hydrothermal method, hydroxyl free radicals are generated by utilizing the peroxide-like property of the Fe-gamma-CD nano material, so that the purpose of bacteriostasis is achieved, and the antibacterial effect is good and harmless to human bodies. The background technology is as follows: Food-borne diseases are a health problem of increasing global concern, and the world health organization indicates that about 70% of human diseases are caused by food-borne pathogens. Common pathogens responsible for food poisoning include E.coli, staphylococcus aureus, salmonella, shigella, and the like. Currently, traditional methods of controlling food-borne bacteria involve physical sterilization techniques and the addition of food additives. For example, heat sterilization techniques are an important method used in food processing. However, this technique generally results in a change in color, aroma, taste of the food product while also reducing the nutritional content. Compared with the traditional heat sterilization technology, the microwave, ultrasonic or pulse electric field can kill harmful bacteria in the food processing process, and meanwhile, the nutrition components are reserved, but the application potential is limited by the higher cost. Sodium benzoate is a widely used food additive. However, the use of sodium benzoate alters the protein structure, thereby inhibiting the function of trypsin and adversely affecting digestion in humans. Thus, there is a strong need for a green, low-toxicity, effective method for controlling food-borne pathogens. At present, lysozyme is a natural biocatalyst, and has strong antibacterial activity against fungi, viruses and bacteria by affecting the permeability of cell membranes, and thus can be used as a preservative in food systems. However, natural enzymes such as lysozyme have inherent disadvantages of high production cost, low catalytic stability, easy inactivation under severe environments, and the like, which greatly limit their potential application as antibacterial agents. With the development of nanotechnology, various nanocomposite materials have been discovered. Wherein, the nano-enzyme is a nano-particle with enzyme catalysis property, and can be used for replacing the traditional antibacterial strategy. Fe 2+ in the artificially synthesized antibacterial agent is a classical catalyst ion widely explored in antibacterial research because it has high POD-like catalytic efficiency and high biocompatibility. It is easily oxidized and thus, it becomes necessary to develop a nanomaterial capable of maintaining its valence state. The high gain et al found that Fe 3O4 nanoparticles had intrinsic peroxidase-like (POD-like) activity, which provided more potential applications for iron-based nanoparticles. In addition, POD-like activity can generate Reactive Oxygen Species (ROS) by reacting with Fenton (Fe 2+) or Fenton-like (Mn 2+,Co3+) of H 2O2 to achieve bacteriostatic effect. The patent with publication number CN116764662A discloses a defect type magnetic FeNi porous carbon nano enzyme with multiple enzyme activities, which takes aquatic waste-waste shrimp shells as raw materials to prepare the defect type magnetic FeNi porous carbon nano enzyme with high specific surface area, large pore capacity and multi-level pore structure, has peroxidase-like activity, hydrogen peroxide-like simulated enzyme activity and oxidase-like activity, and can be used for detecting the total antioxidant level and the total polyphenol content in beverages and fruits in three modes of high-selectivity colorimetric/photothermal/smart phones. The patent with publication number CN102029163A discloses a catalase catalyst, which is prepared by dissolving raw materials of ferric nitrate and bismuth nitrate in ethylene glycol methyl ether, adding a complexing agent and a dispersing agent to prepare sol, further obtaining gel, calcining at high temperature and post-treating to obtain the BiFeO 3 catalase catalyst, can be used as a substitute product of natural catalase, can be used for detecting and analyzing the content of H 2O2 in food, biological and environmental samples, and can also be used for degrading and mineralizing organic pollutants. The invention comprises the following steps: The invention aims to overcome the defects of the prior art and provides a food-grade Fe-gamma-CD nano antibacterial material, a preparation method and application thereof, wherein a gamma-CD ligand in the prepared Fe-gamma-CD nano antibacterial material promotes reduction of Fe 3+ to Fe 2+, so that Fe 2+ and