CN-117282478-B - Fluid control method and device based on micro-fluidic control and micro-fluidic system

Abstract

The application provides a fluid control method, a device and a microfluidic system based on microfluidics, and relates to the technical field of microfluidics, wherein the method comprises the steps of monitoring the current interface position information of a laminar flow interface in a junction flow channel, wherein first liquid flowing out of a first branch flow channel and second liquid flowing out of a second branch flow channel are converged in the junction flow channel to form a laminar flow, and the laminar flow interface is an interface between the first liquid and the second liquid in the junction flow channel; and if the current interface position information is not matched with the target interface position information corresponding to the current working mode, adjusting at least one of the flow rate of the first liquid and the flow rate of the second liquid until the updated current interface position information is matched with the target interface position information so as to form target fluid in the intersection flow passage, wherein the target interface position information is used for indicating the expected position of the laminar flow interface in the intersection flow passage in the current working mode.

Inventors

• TIAN JINGXUAN

Assignees

• 心擎医疗（苏州）股份有限公司

Dates

Publication Date

20260512

Application Date

20230921

Claims (20)

1. 1. The utility model provides a fluid control method based on micro-fluidic, is applied to micro-fluidic device, micro-fluidic device is provided with first branch runner, second branch runner and intersection runner, intersection runner respectively with first branch runner and second branch runner intercommunication, characterized in that, the method includes: Monitoring current interface position information of a laminar flow interface in the intersection flow passage, wherein the first liquid flowing out of the first branch flow passage and the second liquid flowing out of the second branch flow passage are converged in the intersection flow passage to form a laminar flow, and the laminar flow interface is an interface between the first liquid and the second liquid in the intersection flow passage; If the current interface position information is not matched with the target interface position information corresponding to the current working mode, adjusting at least one of the flow rate of the first liquid and the flow rate of the second liquid until the updated current interface position information is matched with the target interface position information so as to form a target fluid in the intersection runner, wherein the target interface position information is used for indicating the expected position of the laminar flow interface in the intersection runner in the current working mode; the target interface position information is determined by the following method: and determining target interface position information corresponding to the current working mode based on a first preset corresponding relation, wherein the first preset corresponding relation is used for representing the corresponding relation between a plurality of working modes of the microfluidic device and preset position information.
2. 2. The method of claim 1, wherein said adjusting at least one of the flow rate of the first liquid and the flow rate of the second liquid to match the updated current interface position information with the target interface position information comprises: determining an offset direction of the laminar flow interface relative to the desired position based on the target interface position information and the current interface position information; And if the offset direction is that the laminar flow interface is offset towards the first branch flow channel side, adjusting at least one of the flow rate of the first liquid and the flow rate of the second liquid so as to adjust the flow rate ratio between the first liquid and the second liquid, and moving the laminar flow interface towards the second branch flow channel side until the updated current interface position information is matched with the target interface position information.
3. 3. The method according to claim 2, wherein the method further comprises: And if the offset direction is that the laminar flow interface is offset towards the second branch flow channel side, adjusting at least one of the flow rate of the first liquid and the flow rate of the second liquid so as to reduce the flow rate ratio between the first liquid and the second liquid until the laminar flow interface moves towards the first branch flow channel side until the updated current interface position information is matched with the target interface position information.
4. 4. The method of claim 1, wherein before said adjusting at least one of the flow rate of the first liquid and the flow rate of the second liquid until the updated current interface position information matches the target interface position information, the method further comprises: and determining the current flow rate of the first liquid based on the current flow rate ratio and the current flow rate of the second liquid.
5. 5. The method of claim 4, wherein said adjusting at least one of the flow rate of the first liquid and the flow rate of the second liquid to match the updated current interface position information with the target interface position information comprises: determining a current flow rate ratio corresponding to the current interface position information and a target flow rate ratio corresponding to the target interface position information based on a second preset corresponding relation, wherein the second preset corresponding relation is used for representing the corresponding relation between the preset flow rate ratio and the plurality of interface position information of the laminar flow interface in the intersection runner; And based on the current flow rate of the second liquid and the current flow rate of the first liquid, performing flow rate adjustment of at least one of the first liquid and the second liquid until the flow rate ratio of the second liquid to the first liquid reaches the target flow rate ratio, so that the updated current interface position information is matched with target interface position information.
6. 6. The method of claim 1, wherein the microfluidic device further comprises a light sensor, a signal acquisition region of the light sensor covers at least a portion of the intersection flow path for generating photosensitive information of laminar flow in the intersection flow path, and wherein the current interface position information is determined by: acquiring photosensitive information acquired by the optical sensor; And carrying out interface position analysis based on the photosensitive information to obtain the current interface position information.
7. 7. The method of claim 6, wherein the light sensor is a camera, the photosensitive information is a runner image collected for the joint runner, and the performing interface position analysis based on the photosensitive information to obtain the current interface position information comprises: Detecting edges of the flow channel walls of the intersection flow channel and a laminar flow interface in the intersection flow channel to obtain a first edge corresponding to a first side wall, a second edge corresponding to a second side wall and a third edge corresponding to the laminar flow interface, wherein the first side wall and the second side wall are two opposite wall surfaces arranged on the intersection flow channel; And determining the current interface position information based on a first distance between the first edge and the third edge and a second distance between the second edge and the third edge.
8. 8. The method of claim 6, wherein the light sensor is a camera, the photosensitive information is a runner image collected for the joint runner, and the performing interface position analysis based on the photosensitive information to obtain the current interface position information comprises: Acquiring coordinate information of a plurality of characteristic points on a demarcation line corresponding to the laminar flow interface based on the flow channel image; determining a slope of the boundary line based on the coordinate information of the plurality of feature points; And determining the current interface position information based on the slope of the dividing line.
9. 9. The method of claim 2, wherein the flow rate ratio is regulated by: Controlling the flow rate of the first liquid in the first branch flow passage to be stable, and adjusting the flow rate of the second liquid in the second branch flow passage to regulate and control the flow rate ratio; And if the flow rate of the second liquid in the second branch flow passage reaches the upper limit or the lower limit of the flow rate of the second liquid, controlling the flow rate of the second liquid in the second branch flow passage to be stable, and regulating the flow rate of the first liquid in the first branch flow passage so as to regulate and control the flow rate ratio.
10. 10. The method of claim 2, wherein the flow rate ratio is regulated by: controlling the flow rate of the second liquid in the second branch flow passage to be stable, and adjusting the flow rate of the first liquid in the first branch flow passage to regulate and control the flow rate ratio; And if the flow rate of the first liquid in the first branch flow passage reaches the upper limit or the lower limit of the flow rate of the first liquid, controlling the flow rate of the first liquid in the first branch flow passage to be stable, and regulating the flow rate of the second liquid in the second branch flow passage so as to regulate and control the flow rate ratio.
11. 11. The method of claim 2, wherein the flow rate ratio is regulated by: if the flow rate ratio between the first liquid and the second liquid needs to be regulated down, controlling the flow rate of the second liquid in the second branch flow passage to be stable, and regulating the flow rate of the first liquid in the first branch flow passage.
12. 12. The method of claim 2, wherein the flow rate ratio is regulated by: If the flow rate ratio between the first liquid and the second liquid needs to be adjusted to be high, the flow rate of the first liquid in the first branch flow passage is controlled to be stable, and the flow rate of the second liquid in the second branch flow passage is adjusted to be low.
13. 13. The method of claim 1, wherein at least one of the first and second branch flow passages communicates with a flow regulating device, and wherein the flow rate of the first liquid or the flow rate of the second liquid is regulated by: And adjusting the operation parameters of the flow adjusting device to adjust the flow rate of the first liquid in a first branch flow passage communicated with the flow adjusting device or the flow rate of the second liquid in a second branch flow passage communicated with the flow adjusting device.
14. 14. The method of any of claims 1-13, wherein the adjusting at least one of the flow rate of the first liquid and the flow rate of the second liquid to match updated current interface position information with target interface position information comprises: Adjusting at least one of the flow rate of the first liquid and the flow rate of the second liquid under a first flow rate constraint condition until the updated current interface position information matches the target interface position information; The first flow rate constraint is used to control the flow rate of the first liquid within a first flow rate range that is a range of fluid speeds required to maintain laminar flow of the first liquid in the first branch flow channel and the junction flow channel, and/or the first flow rate constraint is used to control the flow rate of the second liquid within a second flow rate range that is a range of fluid speeds required to maintain laminar flow of the second liquid in the second branch flow channel and the junction flow channel.
15. 15. The method of any of claims 1-13, wherein the first liquid is blood and the adjusting at least one of the flow rate of the first liquid and the flow rate of the second liquid to match the updated current interface position information with the target interface position information comprises: adjusting at least one of the flow rate of the first liquid and the flow rate of the second liquid under a second flow rate constraint condition until the updated current interface position information matches the target interface position information; The second flow rate constraint condition is for controlling the flow rate of the first liquid within a third flow rate range, the third flow rate range being a fluid speed range required to maintain a laminar flow state of the first liquid in the first branch flow channel and the junction flow channel, and the first liquid not forming a coagulation state.
16. 16. The method of any one of claims 1-13, wherein the microfluidic device further comprises a first detection apparatus and a mixing flow channel in communication with the intersection flow channel, the second liquid being a reaction solution capable of specifically reacting with the first liquid in the current mode of operation in a mixed liquid detection mode, the mixing flow channel being configured to mix the target fluid such that the reaction solution mixes with the first liquid and specifically reacts to form a target mixed liquid; After said adjusting at least one of the flow rate of the first liquid and the flow rate of the second liquid to match the updated current interface position information with target interface position information to form a target fluid in the joint flow channel, the method further comprises: detecting the target mixed liquid based on the first detection equipment to obtain first detection parameter data, wherein the first detection parameter data is used for indicating content information of target substances in the target mixed liquid; and generating a detection result based on the first detection parameter data.
17. 17. The method of claim 16, wherein the target interface position information is determined based on a predetermined mixing ratio between the first liquid and the second liquid when the current operating mode is a mixed liquid detection mode.
18. 18. The method of claim 16, wherein the first liquid is blood; in case the target substance comprises an anticoagulant substance, the first detection parameter data comprises detection data for indicating an anticoagulant level of the blood.
19. 19. The method of claim 16, wherein the method further comprises: Acquiring interface position information corresponding to the target mixed liquid; determining the actual mixing proportion between the first liquid and the second liquid based on the interface position information corresponding to the target mixed liquid; Determining a first liquid dilution ratio corresponding to the target mixed liquid based on the actual mixing proportion; the generating a detection result based on the first detection parameter data includes: the detection result is generated based on the first detection parameter data and the first liquid dilution factor.
20. 20. The method of any one of claims 1-13, wherein the microfluidic device further comprises a second detection apparatus disposed on the joint flow channel, the second liquid being a reference solution; And in the current working mode, namely a cleaning mode aiming at the second detection equipment, the expected position corresponding to the target interface position information is that the laminar flow interface deviates towards the first branch flow passage side and the reference solution covers the detection area of the second detection equipment.

Description

Fluid control method and device based on micro-fluidic control and micro-fluidic system Technical Field The present application relates to the field of microfluidic technologies, and in particular, to a microfluidic-based fluid control method, a microfluidic-based fluid control device, and a microfluidic system. Background Microfluidic refers to a technology for treating or operating micro-fluid by adopting a micro-scale micro-channel, relates to the knowledge intersection of chemistry, fluid physics, microelectronics, new materials, biology and biomedical engineering, and has great development potential and wide application prospect in the fields of biomedical research and the like. In the application of the microfluidic device, in order to ensure the detection effect, the flow velocity of the liquid in the flow channel needs to be accurately regulated so as to meet the detection requirement, but in view of the micro-characteristics of the microfluidic system, the flow velocity detection and flow velocity control difficulty of the liquid to be detected are high, the regulation precision is low, and the detection accuracy is not facilitated. In view of the above, there is a need to provide an improvement to solve at least one of the above-mentioned existing problems. Disclosure of Invention The application provides a fluid control method, a device and a microfluidic system based on microfluidics, which can remarkably reduce the fluid regulation and control precision requirement, regulation and control difficulty and complexity of the microfluidics and improve the detection accuracy and detection efficiency. In one aspect, the present application provides a microfluidic-based fluid control method, applied to a microfluidic device, where the microfluidic device is provided with a first branch flow channel, a second branch flow channel, and a junction flow channel, and the junction flow channel is respectively communicated with the first branch flow channel and the second branch flow channel, and the method includes: Monitoring current interface position information of a laminar flow interface in the intersection flow passage, wherein the first liquid flowing out of the first branch flow passage and the second liquid flowing out of the second branch flow passage are converged in the intersection flow passage to form a laminar flow, and the laminar flow interface is an interface between the first liquid and the second liquid in the intersection flow passage; and if the current interface position information is not matched with the target interface position information corresponding to the current working mode, adjusting at least one of the flow rate of the first liquid and the flow rate of the second liquid until the updated current interface position information is matched with the target interface position information so as to form target fluid in the intersection runner, wherein the target interface position information is used for indicating the expected position of the laminar flow interface in the intersection runner in the current working mode. In a possible implementation manner, the target interface position information is determined by determining target interface position information corresponding to the current working mode based on a first preset corresponding relationship, where the first preset corresponding relationship is used for representing corresponding relationships between a plurality of working modes of the microfluidic device and preset position information. In a possible embodiment, said adjusting at least one of the flow rate of the first liquid and the flow rate of the second liquid to match the updated current interface position information with the target interface position information comprises: determining an offset direction of the laminar flow interface relative to the desired position based on the target interface position information and the current interface position information; And if the offset direction is that the laminar flow interface is offset towards the first branch flow channel side, adjusting at least one of the flow rate of the first liquid and the flow rate of the second liquid so as to adjust the flow rate ratio between the first liquid and the second liquid, and moving the laminar flow interface towards the second branch flow channel side until the updated current interface position information is matched with the target interface position information. In a possible implementation mode, the method further comprises adjusting at least one of the flow rate of the first liquid and the flow rate of the second liquid to reduce the flow rate ratio between the first liquid and the second liquid if the offset direction is that the laminar flow interface is offset towards the second branch flow channel side, and moving the laminar flow interface towards the first branch flow channel side until the updated current interface position information matches with the target