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CN-117299245-B - Modularized integrated pressure-designating quantitative fluid driving chip for instant detection

CN117299245BCN 117299245 BCN117299245 BCN 117299245BCN-117299245-B

Abstract

The invention provides a modularized integrated finger pressure quantitative fluid driving chip for instant detection, which comprises a batch manufacturing method of modularized integrated micro valves on the chip, a reusable finger pressure driving module for quantitatively driving the volume of fluid and a design of a micro-fluidic chip for instant detection. The micro valve is mainly made of silicon rubber and takes an anti-sticking film as a valve body, the finger-pressure driving module comprises a pressing part, a supporting part, a spring and a screw with a pressing head, the instant detection micro-fluidic chip comprises a three-layer structure, the first layer is used for observing detection results, the second layer is used for constructing a fluid channel, and the third layer is used for sampling and constructing a micro pump with the micro valve. According to the invention, the micro valve and the finger pressure driving module are combined on the instant detection micro-fluidic chip, so that mass production of the low-cost micro valve is realized, quantitative driving of fluid is realized by pressing the finger pressure driving module on the chip, the flow control precision of the micro fluid is ensured, and the accuracy of the on-site detection result of the chip is further improved.

Inventors

  • DU ZHICHANG
  • CHEN LING
  • YANG SHAOHUI
  • LIN ZHONGHUA

Assignees

  • 集美大学

Dates

Publication Date
20260512
Application Date
20231106

Claims (9)

  1. 1. The modularized integrated pressure-designating quantitative fluid driving chip for instant detection is characterized by comprising a modularized integrated micro valve and an adjustable quantitative pressure-designating driving module, wherein the modularized integrated micro valve is processed in a standardized way at low cost, the modularized integrated micro valve is embedded into the pressure-designating quantitative fluid driving chip, and the adjustable quantitative pressure-designating driving module is pressed by fingers to realize quantitative driving of multiple fluids on the chip, the adjustable quantitative pressure-designating driving module comprises a pressing part (9), a spring (10), a supporting part (11) and a screw (12), a threaded hole (17) and a spring mounting hole (18) are arranged in the middle of the pressing part (9) so as to be matched with the mounting of the screw (12) and the spring (10), the spring mounting hole (18) corresponding to the pressing part (9) is formed in the supporting part (11), and the top end and the bottom of the screw (12) are respectively provided with a rotary valve (19) and a pressing head (20), wherein when the finger force is applied to the pressing part (9), the spring (10) is compressed, further, the finger is pressed to the maximum pressing amount, and the spring (10) is pressed by the supporting part after the finger is pressed to reach the maximum pressing amount, the spring (11) is pressed by the supporting part, and the same in order to realize quantitative driving of fluid driving each time; The screw (12) can realize the adjustable function of the adjustable quantitative finger pressure driving module by rotating the rotary valve (19) to change the finger pressure stroke and adjust the fluid volume driven by single pressing, the screw (12) can be provided with a plurality of pressing heads (20), and multiple fluids can be driven by one pressing to realize one-source multi-driving.
  2. 2. A modular integrated, designated pressure fluid driver chip for point-of-care testing as recited in claim 1, wherein said modular integrated microvalve is formed by the steps of: the method comprises the steps of 1, pouring a first layer of silicon rubber raw material (1) on a first die (2) and vacuumizing to eliminate bubbles, wherein a large number of semicircular structures (16) are arrayed and distributed on the first die (2); Step 2, placing a cover plate (3) on the first die (2) and the first layer of silicon rubber raw material (1) so as to flatten the first layer of silicon rubber raw material (1) to the same height as the arrayed semicircular ring structures (16), and curing the first layer of silicon rubber raw material (1) under the heat curing condition by vacuumizing; The cover plate (3) is peeled off, the fixed die (4) is horizontally placed on the solidified first layer of silicon rubber raw material, the anti-sticking film (5) is placed in the hollow round holes of the array of the fixed die (4) to cover the solidified first layer of silicon rubber raw material and the semicircular ring structure (16), and the anti-sticking film (5) is used as a valve body part of the micro valve; Step 4, removing the fixed die (4), placing a second die (6) on the solidified first layer of silicone rubber raw material at the position shown in the step 4, pouring the second layer of silicone rubber raw material (101) to a certain height, and then placing a third die (7) into the poured second layer of silicone rubber raw material (101); Step 5, under the heat curing condition, vacuumizing to cure the second layer of silicon rubber raw material (101), and finally sequentially stripping the third die (7), the second die (6) and the first die (2) respectively; and 6, cutting by a tool to obtain a single complete one-way valve (8).
  3. 3. A modular integrated, designated-pressure-quantity fluid-driven chip for on-line detection according to claim 2, characterized in that the first mould (2) comprises a bottom layer and a semicircular ring structure (16) distributed on the bottom layer in an array, the fixed mould (4) is provided with array hollowed-out circular holes cooperating with an anti-adhesive film (5), the second mould (6) is a cylinder with an inner diameter determining the outer dimensions of the non-return valve (8), the third mould (7) comprises a top layer and cylinders distributed on the top layer, in step 3, when the fixed mould (4) is laid flat on the solidified first layer of silicone rubber material, the edge horizontal projection of the holes in the fixed mould (4) is located outside the circumference of the outer edge horizontal projection of the semicircular ring structure (16) and has a spacing such that the anti-adhesive film (5) can be embedded into the non-return valve (8) as a non-return valve body, and when the anti-adhesive film (5) is put into the fixed mould (4), the edge of the anti-adhesive film (5) should coincide with the edge of the fixed mould (4).
  4. 4. A modular integrated, pressure-specific, fluid-driven chip for on-line detection according to claim 2, characterized in that the semicircular ring structure (16), the release film (5), the third die (7) are coaxial.
  5. 5. A modular integrated, designated pressure fluid-driven chip for on-line detection according to claim 2, characterized in that the bottom layer of the first die (2) and the fixed die (4) are both circular and of equal diameter.
  6. 6. A modular integrated, specified-pressure fluid-driven chip for on-line detection according to claim 1, characterized in that the threaded hole (17) is a through hole, the spring mounting holes (18) provided in the support part (11) and the pressing part (9) are the same size, the maximum pressing depth position is the position where the upper surface of the screw (12) is mounted coincident with the lower surface of the support part (11), and the pressing part (9), support part (11), screw (12) are coaxial.
  7. 7. The modular integrated pressure-designating fluid driving chip for instant detection according to claim 1, wherein the pressure-designating fluid driving chip comprises a three-layer structure, namely a bottom layer (13), a first layer of PDMS (14) and a second layer of PDMS (15), wherein the bottom layer (13) is used for observing instant detection results, the first layer of PDMS (14) is provided with a runner and a fluid processing unit for instant detection, a plurality of one-way valve holes (24) are strategically arranged, the one-way valve holes (24) are embedded into a one-way valve standardized by a low-cost method of the modular integrated micro-valve through interference fit, the second layer of PDMS (15) is further provided with a pressure driving chamber (25) unlike the first layer of PDMS (14), wherein fluid flows from a sample inlet (21) of the second layer of PDMS (15), fingers press the pressing head (20) in the finger pressure driving module to transfer force onto the pressure driving chamber (25) of the second layer of PDMS (15), the pressure driving chamber (25) generates a pump film to enable the embedded one-way valve (8) to be selectively mixed with fluid to pass through the one-way valve (14) for instant detection, and finally mix fluid flow (14) is fully opened through the one-way valve (14).
  8. 8. The modularized integrated pressure-designating fluid driving chip for instant detection according to claim 7, wherein the sample injection hole (21), the detection hole (23) and the one-way valve hole (24) are through holes for embedding the one-way valve (8) and injecting and detecting sample reagents, the pressure driving chamber (25) is spaced from the top of the second layer of PDMS (15), the lower surface of the supporting part (11) coincides with the upper surface of the second layer of PDMS (15) before the finger-pressing driving module is pressed, and the pressing head (20) is coaxial with the pressure driving chamber (25).
  9. 9. A modular integrated, defined pressure fluid-driven chip for on-line detection according to claim 7, characterized in that a single pressure-driven chamber (25) is in communication with two of said non-return valves (8) to form a micropump unit (26), and that both of said non-return valves (8) are placed in a positive-negative relationship and integrated on the first layer of PDMS (14) of the chip.

Description

Modularized integrated pressure-designating quantitative fluid driving chip for instant detection Technical Field The invention relates to the technical field of microfluidic chips, in particular to a modularized integrated pressure-designating quantitative fluid driving chip for instant detection. Background The micro-fluidic chip technology is a micro-chip manufactured by utilizing a micro-nano processing technology, and the micro-sample is processed and analyzed by controlling the fluid flow in a micrometer-scale runner. It has great potential in biomedical, chemical analysis, environmental monitoring and other fields and is an indispensable tool in laboratory analysis and biomedical research. The traditional microfluidic chip system is complex, inconvenient to carry, high in cost and slow in response, so that the requirement of on-site instant detection cannot be met, and at the moment, research on driving and controlling of microfluidics is very necessary. Therefore, the invention focuses on the chip integrated micropump technology and fluid driving control method of the microfluidic chip. The chip integrated micropump technology provides rich methods and tools for research and application in the field of microfluidics, and the types of micropumps include electric-driven micropumps, electrochemical-driven micropumps and pressure-driven micropumps. The electric driving micropump can accurately control flow and pressure, but needs a more complex circuit and a control system, the electrochemical driving micropump has a compact structure and higher mobility, but has slower response speed, can be limited by bubble generation and chemical reaction in some application scenes, has a simple structure, is easier to be integrated on a chip in a modularized manner, and meets the driving condition of high-flux fluid. In the finger pressure driven micro-fluidic chip, the flow control method of the pressure driven micro-pump comprises a direct pressing method, an indirect control method and a sample injection control method. The direct pressing method ensures a simple structure of the chip, but can cause larger driving volume errors due to the characteristics of individual fingers (finger force and pressing position), the indirect control method increases the complexity of the chip and has certain limitation on the type of driving fluid under the condition of ensuring certain driving precision when controlling fluid driving by using methods such as pressure transmission by setting an air pressure layer, energy conversion by a piezoelectric element, finger pressure control of capillary force and the like, and the sample injection control method has higher driving precision, but needs higher pressing sensitivity, and the chip relates to complex processing technology. Disclosure of Invention In view of the above, the present invention is directed to a modular integrated finger pressure quantitative fluid driving chip for real-time detection, which utilizes a micro valve integrated on the chip in a modular manner, improves portability of the chip, reduces manufacturing difficulty and processing cost of the micro valve, and makes fluid driving not depend on external devices such as a heavy external pump/power supply to provide input, and a reusable finger pressure driving module for quantitatively driving fluid volume makes driving fluid operation simple, and ensures flow control accuracy of fluid in the chip. The micro valve is integrated on the chip to construct the micro pump by the chip integrated micro pump technology, the finger pressure driving module is used for driving fluid, and the designed micro-fluidic chip can realize simple, rapid and high-precision on-site instant detection by combining the two modules. The modularized integrated pressure-designating quantitative fluid driving chip for instant detection comprises a modularized integrated micro valve and an adjustable quantitative pressure-designating driving module, wherein the modularized integrated micro valve is embedded into the pressure-designating quantitative fluid driving chip, and the adjustable quantitative pressure-designating driving module is pressed by a finger to realize multi-fluid quantitative driving on the chip. In a preferred embodiment, the modular integrated microvalve is low cost, standardized by: the method comprises the steps of 1, pouring a first layer of silicon rubber raw material (1) on a first die (2) and vacuumizing to eliminate bubbles, wherein a large number of semicircular structures (16) are arrayed and distributed on the first die (2); Step 2, placing a cover plate (3) on the first die (2) and the first layer of silicon rubber raw material (1) so as to flatten the first layer of silicon rubber raw material (1) to the same height as the arrayed semicircular ring structures (16), and curing the first layer of silicon rubber raw material (1) under the heat curing condition by vacuumizing; The cover plate (3) is peeled off,