CN-117323284-B - Cosmetic composition comprising fermented velvet flower callus culture extract and mixture of pomegranate-derived exosomes

Abstract

The present invention relates to a composition comprising a mixture of a fermented chenopodium faberi callus culture extract and a pomegranate-derived exosome, and provides a cosmetic composition having more excellent skin moisturizing, anti-inflammatory, anti-aging and wrinkle-improving effects than the use of the fermented chenopodium faberi callus culture extract or the pomegranate-derived exosome alone.

Inventors

• Xu Zailong
• JIN FENGJUN
• Jin Gaoyin
• LIU ZHIXIU
• Chang Elin

Assignees

• 蔻诗曼嘉韩国股份公司
• 蔻诗曼嘉化妆品(中国)有限公司

Dates

Publication Date

20260505

Application Date

20230626

Priority Date

20220630

Claims (7)

1. 1. A cosmetic composition characterized in that, Comprises a mixture of a velvet flower callus culture extract and an exosome derived from punica granatum as an active ingredient; the mixture comprises the chenopodium faberi callus culture extract and the pomegranate-derived exosomes in a weight ratio of 7:3.
2. 2. A cosmetic composition according to claim 1, characterized in that, Comprising from 0.1 to 10.0 weight percent of said mixture, relative to the total weight of the composition.
3. 3. A cosmetic composition according to claim 1, characterized in that, The velvet flower callus culture extract is a fermentation extract obtained by inoculating a lactobacillus strain.
4. 4. A cosmetic composition according to claim 1, characterized in that, The pomegranate source exosome is prepared by the following method: step (a), adding distilled water to the pomegranate pulp raw material and pulverizing to prepare a pulverized product of the pomegranate pulp raw material; step (b), after centrifugal separation of the crushed material, filtering supernatant to separate and remove dead cells and foreign matters; Step (c) of subjecting the filtered supernatant to ultra-high speed centrifugation to form a primary particle layer comprising exosomes; A step (d) of subjecting the supernatant, which has been subjected to the centrifugation in the step (c), to centrifugation again to form a secondary particle layer containing exosomes, and And (e) separating exosomes from the pomegranate pulp by re-suspending the primary and secondary particle layers in purified water.
5. 5. A cosmetic composition according to claim 4, wherein, Repeating the steps (c) and (d) 2 to 3 times to isolate the exosomes of high purity.
6. 6. The cosmetic composition according to any one of claim 1 to 5, characterized in that, The composition is formulated into one or more of emollient water, nourishing lotion, moisturizing cream, nourishing cream, massage cream, essence, ampoule, gel, eye cream, cleansing cream, facial cleanser, cleansing water, facial mask, spray, and powder.
7. 7. The cosmetic composition according to any one of claim 1 to 5, characterized in that, The composition is used for moisturizing skin, anti-inflammatory, anti-aging or improving skin wrinkles.

Description

Cosmetic composition comprising fermented velvet flower callus culture extract and mixture of pomegranate-derived exosomes Technical Field The present invention relates to a cosmetic composition comprising a fermented edelweiss callus culture extract and a mixture of exosomes derived from punica granatum, which has effects of moisturizing skin, anti-inflammatory, anti-aging or improving wrinkles. Background Anti-inflammatory, whitening, skin moisturizing and anti-wrinkle are always a focus of attention as a beautiful and anti-aging mark. With this concern, products for whitening, moisturizing and anti-aging skin are actively being introduced in the cosmetic market, and as consumer interest increases, demand is increasing, and related cosmetic industries are growing into high added value industries. Recently, according to many studies, many materials have been developed which can maximize not only an anti-inflammatory effect and an effect of promoting skin moisturizing ability but also an anti-wrinkle effect by changing the activity of a collagen synthesis-related element that generates wrinkles, and the demands of research and consumers have increased. Endoplasmic reticulum means a microcapsule having a membrane structure with a diameter of about 20nm to 5 μm, and is composed of exosomes (exosomes), exonuclear granules (ectosome), microbubbles (microvesicle), microparticles (microparticle) and the like according to the size and composition thereof. Wherein exosomes are substances secreted to the outside of cells in all living cells such as animal cells, microorganisms, plants and body fluids as means of intercellular communication, and are substances with a size of 50nm to 200nm nanometers. Exosomes consist of phospholipid bilayers resembling cell membranes, which efficiently and easily transmit corresponding signals inside the cell by transmitting proteins to the target cells, and which can complex signals because a variety of secondary metabolites and substances related to genetic information can be delivered at once. But has the disadvantages of high manufacturing cost and poor price competitiveness. In addition, in the development of conventional cosmetic materials, plant cells cultured from velvet flowers in plant resources, adventitious root cultures, or extracts thereof are used, and fermentation processes are also performed. Unlike these prior studies, however, little research has been done on efficacy differences based on exosome mixing ratios. Thus, in the present invention, it was confirmed that in the case of preparing a complex by mixing a fermented chenopodium faberi callus culture extract and a pomegranate-derived exosome, wrinkle improvement, skin moisturizing, anti-inflammatory effects can be promoted and enhanced as compared to the case of using the fermented chenopodium faberi callus culture extract or the pomegranate-derived exosome alone, thereby completing the present invention. Prior art literature Patent literature Korean laid-open patent publication No. 10-2016-0043551 Korean patent publication No. 10-1842700 Disclosure of Invention Problems to be solved by the invention The present invention is directed to a cosmetic composition comprising a mixture of a fermented chenopodium faberi callus culture extract and an exosome derived from punica granatum as active ingredients, and having effects of improving skin moisture, anti-inflammatory, anti-aging and improving wrinkles. Solution for solving the problem In order to achieve the above-described object, the present inventors have confirmed that when a fermented velvet flower callus culture extract and a pomegranate-derived exosome are used in a mixture in a certain ratio, the results thereof show that the effects of improving wrinkles, skin moisturizing, and anti-inflammatory effects and the like can be improved, and that the effects of improving wrinkles, skin moisturizing, and anti-inflammatory effects are improved as compared to the use of a velvet flower callus culture extract or a pomegranate-derived exosome alone, thereby completing the present invention. The present invention provides a cosmetic composition comprising a mixture of a callus culture extract of chenopodium faberi (Leontopodium alpinum) and an exosome derived from pomegranate pulp as active ingredients. The cosmetic composition of the present invention contains a natural extract as an active ingredient, not only has excellent biosafety, but also minimizes skin irritation. In particular, the plant cell culture extract of the velvet flower and the exosome derived from the pomegranate act on the skin at the same time, so that the synergistic effect of the plant cell culture extract and the exosome derived from the pomegranate can obviously enhance the functions of anti-inflammation, anti-aging, wrinkle improvement, skin moisture preservation and the like. The above-mentioned mixture of fermented edelweiss callus culture extract and pomegranate-derived exosomes can be m