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CN-117343404-B - Conductive hydrogel material and preparation method and application thereof

CN117343404BCN 117343404 BCN117343404 BCN 117343404BCN-117343404-B

Abstract

The invention discloses a conductive hydrogel material and a preparation method and application thereof. The preparation method comprises the steps of carrying out Michael addition reaction and cross-linking reaction on a first high molecular compound and a second high molecular compound to obtain a hydrogel precursor, compressing and mixing the hydrogel precursor and a conductive carbon-based material in a die, standing, and demolding to obtain the conductive hydrogel material. According to the preparation method disclosed by the invention, the conductive carbon-based material and the hydrogel precursor are mixed in the mould, so that the conductivity of the carbon-based conductive hydrogel prepared by the traditional method is improved, and the carbon-based conductive hydrogel has wider application space in the field of biological sensing. Meanwhile, the conductive hydrogel material prepared by the invention has good mechanical property and certain adhesiveness, is safe in hydrogel component and good in biocompatibility, can be used as a biological scaffold material to better simulate the cell growth environment, can realize the regulation and control of cell growth through external stimulation, has good mechanical property and pressure sensitivity, and is suitable for being used as a flexible biological sensing electrode material.

Inventors

  • LI JIAWEI
  • HAO YING
  • CHENG GUOSHENG
  • HONG JING

Assignees

  • 中国科学院苏州纳米技术与纳米仿生研究所

Dates

Publication Date
20260512
Application Date
20231110

Claims (11)

  1. 1. A method for preparing a conductive hydrogel material, comprising: Mixing a first solution containing a first high molecular compound which is a combination of any one or more of collagen, hyaluronic acid and gelatin modified by at least one of dopa, dopamine and tannic acid and a second solution containing a second high molecular compound which is a combination of any one or more of polyethylene glycol and polyvinyl alcohol modified by at least one of acrylic acid, methacrylic acid and butenoic acid, and performing a Michael addition reaction and a crosslinking reaction on the first high molecular compound and the second high molecular compound to prepare a hydrogel precursor solution; Compacting the conductive carbon-based material in a mould, then mixing the compacted conductive carbon-based material with a hydrogel precursor solution, standing, and demoulding to obtain the conductive hydrogel material, wherein the conductive carbon-based material is a carbon nano tube.
  2. 2. The method of claim 1, wherein the carbon nanotubes are multi-walled carbon nanotubes having an outer diameter of less than 2nm and a length of 5-30 μm.
  3. 3. The method of claim 1, wherein the mass ratio of the first polymer compound to the second polymer compound is 1:1-3:1.
  4. 4. The method of claim 1, wherein the pH of the first solution or the second solution is 7.7-8.0.
  5. 5. The method of claim 1, wherein the standing time is 5 to 15 minutes.
  6. 6. A conductive hydrogel material produced by the production method of any one of claims 1 to 5.
  7. 7. The conductive hydrogel material of claim 6, wherein the conductive carbon-based material is present in an amount of 10 to 40wt%.
  8. 8. The conductive hydrogel material of claim 6, wherein the conductive hydrogel material has an electrical conductivity of 5-20S/m.
  9. 9. Use of the electrically conductive hydrogel material of any one of claims 6-8 in the field of biomaterials, biosensing or wearable devices.
  10. 10. The use according to claim 9, wherein the biological material comprises a flexible biosensing electrode material.
  11. 11. A conductive hydrogel electrode material comprising the conductive hydrogel material of any one of claims 6-8.

Description

Conductive hydrogel material and preparation method and application thereof Technical Field The invention relates to a hydrogel material, in particular to a conductive hydrogel material, and a preparation method and application thereof, and belongs to the technical field of new nano materials. Background The hydrogel is a flexible material with a three-dimensional network structure, which is formed by taking water as a medium and crosslinking a macromolecular material containing hydrophilic groups through intermolecular covalent bonds, hydrogen bonds and other bond sites. The carbon-based material becomes an ideal conductive filler of the conductive hydrogel due to unique properties such as high conductivity, large specific surface area and the like. The hydrogel scaffold material can imitate natural tissues in structure and function, can provide adhesion sites and three-dimensional growth spaces for cells, and is beneficial to information transmission among cells and transmission of nutrient substances and metabolites. Meanwhile, the hydrogel scaffold material also has good biocompatibility, highly imitates in vivo microenvironment, can maintain cell phenotype, and is beneficial to guiding formation of nerve tissues. Meanwhile, compared with the traditional rigid electronic sensor, the flexible wearable sensor prepared by combining the hydrogel material has the characteristics of greater flexibility, ductility, small volume and the like, and can convert pressure, deformation and the like into recordable electronic signals. The conductive hydrogel has the dual characteristics of conductive substances and hydrogel, and can be applied to the fields of tissue engineering materials, biosensing and the like. CN113308148a provides a preparation method of a conductive hydrogel ink, wherein the conductive hydrogel component comprises polyvinyl alcohol, carbon nano-tube, sodium carboxymethyl cellulose and the like. The mechanical strength of the hydrogel is improved by the preparation method of cyclic freezing and thawing. CN110746615B provides a preparation method and application of a pH-responsive high-strength conductive hydrogel, and the hydrogel prepared by the method has high strength and can be safely used as a carrier of a drug. However, the conductivity of the conductive hydrogel prepared by the method is low, and the application of the conductive hydrogel is limited to a certain extent. CN114349980a provides a conductive hydrogel, a preparation method and application thereof, wherein the conductive hydrogel is composed of sodium alginate, unsaturated monomers and metal ions, and the conductive hydrogel prepared by the invention has good conductivity, ductility and high sensitivity, and can be applied to preparing sensors. However, the gel-forming and solidifying time is long, and the biocompatibility is not good due to the existence of metal ions, so that the application of the gel-forming and solidifying agent in the aspect of biosensing is limited to a certain extent. In summary, most of the researches at present mainly include crosslinking a conductive polymer and a high molecular material by a physical or chemical method to prepare the conductive composite hydrogel scaffold material. Although this gives the conductive composite stent unique properties of both biopolymer and conductive polymer. However, the defects of low conductivity, single structure, poor flexibility, poor biocompatibility and the like of the conductive hydrogel stent are difficult to solve well so as to meet the requirements of tissue engineering application. Therefore, the conductive hydrogel material with high conductivity, good biocompatibility and rich space structure is a problem to be solved. Disclosure of Invention The invention mainly aims to provide a conductive hydrogel material and a preparation method thereof, so as to overcome the defects in the prior art. It is a further object of the present invention to provide the use of said electrically conductive hydrogel material. In order to achieve the aim of the invention, the invention adopts the following technical scheme: the embodiment of the invention provides a conductive hydrogel material, which is prepared by doping conductive carbon-based materials after Michael addition reaction and crosslinking reaction of a first high molecular compound and a second high molecular compound. In some embodiments, the first polymer compound includes any one or a combination of two or more of collagen, hyaluronic acid, and gelatin, but is not limited thereto. In some embodiments, the second polymer compound includes any one or a combination of two of polyethylene glycol and polyvinyl alcohol, but is not limited thereto. The embodiment of the invention also provides a preparation method of the conductive hydrogel material, which comprises the following steps: The first polymer compound and the second polymer compound are subjected to Michael addition reaction and crosslinking reaction