CN-117358331-B - Liquid drop preparation system

CN117358331BCN 117358331 BCN117358331 BCN 117358331BCN-117358331-B

Abstract

The invention relates to a liquid drop preparation system which comprises a liquid drop chip, an execution unit and a light solidifying device, wherein the liquid drop chip is used for preparing a liquid phase and an oil phase into liquid drops, the execution unit is used for injecting the liquid phase and the oil phase into the liquid drop chip, the execution unit comprises a first actuator, a second actuator and a needle tube, the first actuator and the second actuator are identical in structure, the first actuator is detachably connected with the needle tube, the second actuator is detachably connected with the needle tube, the first actuator and the second actuator respectively push the needle tube, the controller is used for controlling the operation of the execution unit and the injection speed of the liquid phase and the oil phase, the light solidifying device is used for accelerating the solidification of the liquid drops, the controller is connected with the execution unit through a signal line, the execution unit is connected with the liquid drop chip through a pipeline, the liquid drop chip is hollow in the pipeline, and the liquid can pass through the pipeline. The above arrangement enables the preparation of droplets and the photocuring of droplets of different compositions.

Inventors

Shu xiong
CHEN LEI
QI HUI
JIE YONGSHENG
ZHENG RUI

Assignees

北京市创伤骨科研究所

Dates

Publication Date: 20260505
Application Date: 20231025

Claims (9)

1. A droplet preparation system, comprising: the liquid drop chip is used for preparing the water phase and the oil phase into liquid drops; The device comprises a liquid drop chip, an execution unit, a first actuator, a second actuator and a needle tube, wherein the execution unit is used for injecting a water phase and an oil phase into the liquid drop chip and comprises a first actuator, a second actuator and the needle tube; a controller for controlling the operation of the execution unit and the injection speed of the water phase and the oil phase; The photocuring device accelerates the solidification of the liquid drops, so that the solidification process of the liquid drops is stable, the solidification is complete, microspheres are not fused, the photocuring device comprises a pipeline placement structure for placing pipelines for experiments, heating and temperature controlling the pipelines, the placement length of the pipelines in the pipeline placement structure is adjustable, the upper surface of the pipeline placement structure is provided with a pipeline channel, the pipeline channel comprises a straight line section and a curved section, the straight line section comprises a first straight line section and a second straight line section, the first straight line section is arranged at a position close to the width edge and is parallel to the width direction of the pipeline placement structure, the first straight line section is communicated with the second side surface of the pipeline placement structure and the curved section, at least two curved sections are arranged, the curved sections are coiled into a plurality of circles, the inner circle of the first curved section is connected with the second curved section, the innermost circle of the curved section is connected with the second straight line section, the second straight line section is parallel to the first inner circle, and the second straight line section is connected with the first side surface of the curved section; the controller is connected with the execution unit through a signal line, the execution unit is connected with the liquid drop chip through a pipeline, the liquid drop chip is connected with the light curing device through a pipeline, the pipeline is hollow, and liquid passes through the pipeline.
2. The liquid drop preparation system of claim 1, wherein the liquid drop chip is internally provided with a first channel and a second channel, the second end of the first channel is connected with the second end of the second channel and then combined into a total channel, and the first ends of the first channel and the second channel and the tail ends of the total channel are respectively provided with a connecting hole.
3. The droplet preparation system of claim 1 wherein the first and second actuators are each coupled to a controller.
4. The liquid drop preparation system according to claim 3, wherein the first actuator comprises a motor, a rectangular plate, a screw rod and a movable block, the rectangular plate comprises a first plate, a second plate and a third plate, the motor is separated from the first plate and fixedly connected to the first plate, a shaft of the motor is fixedly connected with the screw rod, the movable block is movably connected with the screw rod, the second plate is arranged between the first plate and the third plate, two polished rods are arranged between the first plate and the third plate, the motor is connected with the screw rod through a coupler, the movable block is rectangular in shape, a first threaded hole is formed in the center of any side face of the movable block, the first threaded hole is matched with threads on the screw rod, and the movable block is arranged on the screw rod and the polished rod.
5. The liquid drop preparation system according to claim 4, wherein a notch is formed in the top of the movable block, an opening is formed in any side of the notch at the top of the movable block, a second threaded hole is formed in the side face, opposite to the opening, of the movable block, a clamping block is arranged in the notch, a threaded rod is arranged on the side face of the clamping block, the first end of the threaded rod is fixedly connected with the clamping block, the threaded rod is matched with the second threaded hole, the threaded rod penetrates through the second threaded hole, an operating portion is arranged at the second end of the threaded rod, the operating portion is fixedly connected with the threaded rod, and an anti-slip layer is arranged on the operating portion.
6. The drop preparation system of claim 4, wherein a circular groove is formed in the center of the top of the third plate, a fixed plate is arranged on the top of the third plate, the fixed plate spans the circular groove, and the fixed plate is movably connected with the third plate.
7. The liquid drop preparation system of claim 1, wherein the light curing device further comprises a baffle plate disposed around the upper surface of the pipeline placement structure, the baffle plate being fixedly connected to the pipeline placement structure for protecting the pipeline from the external environment, and a light source structure disposed on the upper portion of the baffle plate and rotatably connected to the baffle plate.
8. The droplet preparation system according to claim 7, wherein the conduit placement structure is internally provided with heating wires.
9. The droplet preparation system according to any one of claims 7-8, wherein the light source structure comprises a top cover and an illumination lamp, the illumination lamp is fixedly connected to the bottom of the top cover, and at least one illumination lamp is arranged on the top cover.

Description

Liquid drop preparation system Technical Field The invention belongs to the technical field of medical instruments, and particularly relates to a liquid drop preparation system. Background The micro-droplet has important application in the fields of drug controlled release, virus detection, particle material synthesis, catalyst and the like. In the IVD field, micro droplets are used for gene chips, protein chips and dPCR. The development of microfluidic technology realizes controllable design and accurate control of the size, structure, morphology and function of micro-droplets, and becomes one of the most important technical platforms in the field of micro-droplets. The microfluidic method is mainly to prepare micro liquid drops by using two mutually incompatible liquids as continuous phase and discrete phase respectively, and controlling the generation of liquid drops by controlling the microtubule structure and the two-phase flow rate ratio. The continuous phase and the discrete phase enter different micro-channels respectively under the pushing of a driving pump with fixed volume flow rate, after two fluids meet at an intersection point, the discrete phase fluid continues to extend to form a plug-shaped or spray-shaped liquid column, and then is pinched off under the shearing and extrusion actions of the continuous phase fluid, and is dispersed in the continuous phase in the form of micro-droplets. The existing droplet preparation system has the problems that the curing process of micro droplets is unstable, and the fusion or the curing of microspheres are incomplete. The present invention addresses the above-described problems by providing a droplet preparation system. Disclosure of Invention In order to overcome the problems in the background art, the invention adopts the following technical scheme: A droplet preparation system, comprising: the liquid drop chip is used for preparing the water phase and the oil phase into liquid drops; The device comprises a liquid drop chip, an execution unit, a first actuator, a second actuator and a needle tube, wherein the execution unit is used for injecting a water phase and an oil phase into the liquid drop chip and comprises a first actuator, a second actuator and the needle tube; a controller for controlling the operation of the execution unit and the injection speed of the water phase and the oil phase; The photocuring device accelerates the curing of the liquid drops, so that the curing process of the micro liquid drops is stable, the curing is complete, and the microspheres are not fused; The controller is connected with the execution unit through a signal line, the execution unit is connected with the liquid drop chip through a pipeline, the liquid drop chip is connected with the light curing device through a pipeline, the pipeline is hollow, and liquid passes through the pipeline. The above arrangement enables the preparation of droplets and the photocuring of droplets of different compositions. Further, a first channel and a second channel are arranged in the liquid drop chip, the second end of the first channel is connected with the second end of the second channel and then combined into a total channel, and an oil phase and a water phase enter the first channel and the second channel respectively to form liquid drops in the total channel. Further, the first ends of the first channel, the second channel and the tail end of the total channel are respectively provided with a connecting hole, and the connecting holes can be connected with pipelines. The first actuator and the second actuator are respectively connected with the controller, and the controller can respectively control the pushing speed of the first actuator and the second actuator to the needle tube, so that the formation of liquid drops is facilitated. The first actuator comprises a motor, a rectangular plate, a screw and a movable block, wherein the rectangular plate comprises a first plate, a second plate and a third plate, the motor is separated from the first plate and fixedly connected to the first plate, a shaft of the motor is fixedly connected with the screw, and the movable block is movably connected with the screw. Further, first board, second board and third board center set up first through-hole, and the second board sets up between first board and third board, and the first through-hole both sides of second board set up the second through-hole respectively, set up two polished rods between first board and the third board, the polished rod sets up the both sides at first through-hole, polished rod diameter is the same with second through-hole diameter, and the polished rod passes the second through-hole. Further, the motor is connected with the screw rod through a coupler. Furthermore, the bottoms of the adjacent side surfaces of the second plate and the third plate are provided with a bottom plate which is fixedly connected with the second plate and the third plate respectively,