Search

CN-117384224-B - Method for extracting beta-nicotinamide mononucleotide from microalgae and microalgae extract

CN117384224BCN 117384224 BCN117384224 BCN 117384224BCN-117384224-B

Abstract

The present disclosure relates to a method for extracting beta-nicotinamide mononucleotide from microalgae and a microalgae extract. The method comprises the steps of mixing microalgae raw materials with an extracting agent for extraction treatment, wherein the microalgae raw materials are one or more of chlorella, spirulina and haematococcus pluvialis, and the extracting agent comprises 0.05-1.5 wt% of nonionic surfactant, 0.5-1.0 wt% of organic acid, 20-40 wt% of alcohol and the balance of solvent. The microalgae raw material is easy to culture, has high growth speed, high protein content and high beta-nicotinamide mononucleotide content, and the extraction method is simple, high in extraction rate and low in cost.

Inventors

  • GUO BAOWEN
  • ZONG BAONING
  • RONG JUNFENG
  • LI XIU
  • ZHU JUNYING

Assignees

  • 中国石油化工股份有限公司
  • 中国石油化工股份有限公司石油化工科学研究院

Dates

Publication Date
20260505
Application Date
20220705

Claims (14)

  1. 1. A method for extracting beta-nicotinamide mononucleotide from microalgae is characterized by comprising the steps of mixing microalgae raw materials with an extracting agent for extraction treatment; wherein the microalgae raw material is selected from one or more of chlorella, spirulina and haematococcus pluvialis; The extractant comprises 0.05-1.5 wt% of nonionic surfactant, 0.5-1.0 wt% of organic acid, 20-40 wt% of alcohol and the balance of solvent; The nonionic surfactant is one or two selected from tea saponin and sitosterol, the organic acid is one or more selected from citric acid, acetic acid and formic acid, the alcohol is one or more selected from methanol and n-propanol, and the solvent is water.
  2. 2. The method of claim 1, wherein the microalgae feedstock is selected from the group consisting of chlorella.
  3. 3. The method of claim 2, wherein the microalgae feedstock is chlorella pyrenoidosa.
  4. 4. The method of claim 1, wherein the microalgae raw material is used in a form comprising dry algae powder and fresh algae mud.
  5. 5. The method of claim 1, wherein the extractant comprises 0.5 to 1.5 wt% nonionic surfactant, 0.7 to 1.0 wt% organic acid, 30 to 40 wt% alcohol, and the balance solvent.
  6. 6. The method according to claim 1, wherein the conditions of the extraction treatment include an amount of the extractant of 20 to 40mL, an extraction temperature of 30 to 60 ℃ and an extraction time of 30 to 60min relative to 1g of the microalgae raw material.
  7. 7. The method according to claim 6, wherein the conditions of the extraction treatment include that the extraction treatment is carried out under ultrasonic conditions, and the ultrasonic power is 200-400W.
  8. 8. The method according to claim 6, wherein the conditions of the extraction treatment include an amount of the extractant of 30 to 40mL, an extraction temperature of 40 to 60 ℃ and an extraction time of 30 to 40min relative to 1g of the microalgae raw material.
  9. 9. The method of claim 7, wherein the conditions for the extraction process include an ultrasonic power of 300-400W.
  10. 10. The method according to claim 1, further comprising centrifuging the product obtained by the extraction treatment, and then taking a supernatant from the centrifugation and performing ultrafiltration to obtain a filtrate; and (3) performing freeze drying treatment on the filtrate.
  11. 11. The method according to claim 10, wherein the centrifugation conditions include a centrifugation speed of 5000-10000 rpm and a centrifugation time of 5-15 min.
  12. 12. The method according to claim 11, wherein the centrifugation conditions include a centrifugation speed of 8000 to 10000rpm and a centrifugation time of 5 to 10 minutes.
  13. 13. The method of claim 10, wherein the ultrafiltration treatment is performed by using an ultrafiltration membrane having a molecular weight cut-off of 1-5 kd.
  14. 14. The method of claim 13, wherein the ultrafiltration membrane has a molecular weight cut-off of 1 to 2kd.

Description

Method for extracting beta-nicotinamide mononucleotide from microalgae and microalgae extract Technical Field The present disclosure relates to the field of natural microorganism extraction technology, and in particular, to a method for extracting β -nicotinamide mononucleotide from microalgae and a microalgae extract. Background Beta-Nicotinamide Mononucleotide (NMN), which is the product of the nicotinamide riboside transferase reaction, is one of the key precursors for NAD+ synthesis. Nad+ is a key coenzyme for cellular energy metabolism and oxidative stress adaptation, and nad+ centered mammalian senescence theory suggests that nad+ levels determine the rate and extent of senescence. NAD+ is an important cofactor or co-substrate in all cells of the human body and can be used in a variety of enzymatic processes including glycolysis, TCA cycle, oxidative phosphorylation, DNA repair, protein deacetylation, etc. Nad+ levels are important for maintaining mitochondrial homeostasis, organism metabolism, normal function of organs and tissues, and for delaying aging. Currently, NMN is mainly prepared by chemical synthesis, enzymatic synthesis and microbial fermentation. But the synthesis is difficult, the cost is high, and the like. NMN is widely present in natural foods, such as emulsions of fruits, vegetables and mammals, and the like, and the literature reports that the NMN content in fresh samples of 100g of avocados, cabbages and broccoli is 6.519 mug, 4.091 mug and 2.30 mug respectively, but the problem of lower extraction rate of beta-nicotinamide mononucleotide exists when the natural foods are used for extracting the beta-nicotinamide mononucleotide. Disclosure of Invention The purpose of the present disclosure is to provide a method for extracting beta-nicotinamide mononucleotide from microalgae, which has simple process and high extraction rate of beta-nicotinamide mononucleotide, and a microalgae extract. In order to achieve the above object, a first aspect of the present disclosure provides a method for extracting β -nicotinamide mononucleotide from microalgae, comprising mixing microalgae raw materials with an extracting agent for extraction treatment, wherein the microalgae raw materials are selected from one or more of chlorella, spirulina and haematococcus pluvialis, and the extracting agent comprises 0.05-1.5 wt% of nonionic surfactant, 0.5-1.0 wt% of organic acid, 20-40 wt% of alcohol and the balance of solvent. Optionally, the microalgae raw material comprises chlorella, more preferably chlorella pyrenoidosa, and optionally, the microalgae raw material comprises dry algae powder and fresh algae mud. Optionally, the extractant comprises 0.5-1.5 wt% of nonionic surfactant, 0.7-1.0 wt% of organic acid, 30-40 wt% of alcohol and the balance of solvent. Optionally, the nonionic surfactant is selected from one or two of tea saponin and sitosterol, the organic acid is selected from one or more of citric acid, acetic acid and formic acid, the alcohol is selected from one or more of methanol and n-propanol, and the solvent is water. Optionally, the conditions of the extraction treatment comprise that the dosage of the extractant is 20-40 mL, the extraction temperature is 30-60 ℃ and the extraction time is 30-60 min relative to 1g of the microalgae raw material, and optionally, the extraction treatment is carried out under the ultrasonic condition, and the ultrasonic power is 200-400W. Optionally, the conditions of the extraction treatment comprise the use amount of the extractant is 30-40 mL, the extraction temperature is 40-60 ℃, the extraction time is 30-40 min, and the ultrasonic power is 300-400W relative to 1g of the microalgae raw material. Optionally, the method further comprises centrifuging the product obtained by the extraction treatment, taking the supernatant from the centrifugation, ultrafiltering to obtain filtrate, and lyophilizing the filtrate. Optionally, the centrifugation conditions comprise a centrifugation speed of 5000-10000 rpm, preferably 8000-10000 rpm, a centrifugation time of 5-15 min, preferably 5-10 min, optionally, an ultrafiltration membrane is adopted in the ultrafiltration treatment, and the molecular weight cut-off of the ultrafiltration membrane is 1-5 kD, preferably 1-2 kD. A second aspect of the present disclosure provides a microalgae extract obtained according to the method of the first aspect of the present disclosure, the microalgae extract comprising β -nicotinamide mononucleotide. The microalgae extract is a microalgae extract, wherein the microalgae extract comprises beta-nicotinamide mononucleotide and the extractant, the content of the beta-nicotinamide mononucleotide is 2-200 mg/L based on the volume of the microalgae extract, and preferably, when the microalgae raw material is chlorella, the content of the beta-nicotinamide mononucleotide is 100-200 mg/L based on the volume of the microalgae extract. Through the technical scheme, the method for extracting