CN-117445491-B - Composite method of protein film and composite protein film

Abstract

The application relates to the technical field of protein film preparation, in particular to a protein film compounding method and a compound protein film. The method comprises the steps of providing a first protein film and a second protein film, wherein the first protein film is a protein film after cross-linking treatment, bonding one surface of the first protein film with one surface of the second protein film to obtain a bonded protein film, applying a protein solution between the bonded surfaces of the first protein film and the second protein film in the bonded protein film, and carrying out cross-linking treatment on the bonded protein film so that the protein solution connects the first protein film and the second protein film together through cross-linking reaction to obtain the composite protein film. By attaching two protein films together and applying protein solution between the attached protein films, multiple layers of protein films can be compounded together in a secondary crosslinking mode, and the regulation and control of the deformation performance of the compound protein film when meeting water are realized, so that the application of the protein film is expanded.

Inventors

• TAO HU
• LIU KEYIN

Assignees

• 江西丝科生物科技有限公司

Dates

Publication Date

20260512

Application Date

20231206

Claims (10)

1. 1. A method for complexing a protein film, comprising: providing a first protein film and a second protein film, wherein the first protein film is a protein film after cross-linking treatment; Attaching one surface of the first protein film to one surface of the second protein film to obtain an attached protein film, wherein a protein solution is applied between the attached surfaces of the first protein film and the second protein film in the attached protein film; And carrying out crosslinking treatment on the attached protein film so that the protein solution connects the first protein film and the second protein film together through crosslinking reaction to obtain the composite protein film.
2. 2. The method of claim 1, wherein the second protein film is a cross-linked protein film, wherein the first protein film and the second protein film are both wet protein films, wherein the bonding the one surface of the first protein film to the one surface of the second protein film to obtain a bonded protein film comprises: applying a protein solution to one surface of the first protein film; And attaching one surface of the second protein film to the surface of the first protein film to which the protein solution is applied, so as to obtain the attached protein film.
3. 3. The method of claim 1, wherein the first protein film is a wet protein film and the second protein film is a dry protein film that has not been cross-linked, wherein the bonding one surface of the first protein film to one surface of the second protein film to obtain a bonded protein film comprises: And attaching one surface of the first protein film to one surface of the second protein film, so that the surface part of the second protein film attached to the first protein film is dissolved to form a protein solution, and the attached protein film is obtained.
4. 4. The method of claim 1, wherein the second protein film is a cross-linked protein film, and the first protein film and the second protein film are both dry protein films, and wherein the bonding one surface of the first protein film to one surface of the second protein film to obtain a bonded protein film comprises: applying a protein solution to one surface of the first protein film; And attaching one surface of the second protein film to the surface of the first protein film to which the protein solution is applied, so as to obtain the attached protein film.
5. 5. The method of claim 1, wherein the second protein film is a cross-linked protein film, and the first protein film and the second protein film are both dry protein films, and wherein the bonding one surface of the first protein film to one surface of the second protein film to obtain a bonded protein film comprises: Plasma treating one surface of the first protein film and one surface of the second protein film, respectively; applying a protein solution to the plasma treated surface of the first protein film; And attaching the surface of the second protein film subjected to plasma treatment to the surface of the first protein film to which the protein solution is applied, so as to obtain the attached protein film.
6. 6. The method according to any one of claims 1 to 5, wherein the protein in the protein solution, the protein forming the first protein film, and the protein forming the second protein film are the same in kind.
7. 7. The method of claim 6, wherein the protein in the protein solution is fibroin.
8. 8. The method of compounding a protein film according to claim 1, wherein the cross-linking the laminated protein film to join the first protein film and the second protein film together by a cross-linking reaction of the protein solution to obtain a composite protein film comprises: extruding the two side surfaces of the attached protein film, and drying the attached protein film; And placing the dried attached protein film in a water vapor atmosphere for crosslinking treatment so that the protein solution connects the first protein film and the second protein film together through crosslinking reaction to obtain the composite protein film.
9. 9. The method of claim 8, wherein the step of crosslinking the dried laminated protein film in a water vapor atmosphere to allow the protein solution to join the first protein film and the second protein film together by a crosslinking reaction to obtain a composite protein film comprises: Placing the dried attached protein film in a steam atmosphere for crosslinking treatment so that the protein solution connects the first protein film and the second protein film together through crosslinking reaction to obtain a combined protein film; and drying the combined protein film to obtain the composite protein film.
10. 10. A composite protein film, characterized in that the composite protein film is prepared by a composite method based on the protein film according to any one of claims 1 to 9.

Description

Composite method of protein film and composite protein film Technical Field The application relates to the technical field of protein film preparation, in particular to a silk protein film compounding method and a compound protein film. Background The protein film is a material with excellent biocompatibility, has the advantages of difficult immune response initiation, degradability, drug loading and the like, and becomes a preferred choice for manufacturing medical devices. Moreover, the protein film may also serve as a substrate for flexible electronic devices, such as transient soluble electronic devices, and the like. Currently, most protein films are typically monolayer films. However, the mechanical properties of a single-layer protein film are limited, and the regulation and control of the internal stress of the protein film cannot be realized, which limits the application of the protein film. In addition, in flexible electronic devices, a single layer of protein film is often only capable of being used as a substrate for the flexible electronic device, and packaging of the flexible electronic device cannot be achieved by using the protein film. Disclosure of Invention In order to solve the technical problems, the application provides a compounding method of a protein film and the compound protein film. In a first aspect, an embodiment of the present application discloses a method for compounding a protein film, including: Providing a first protein film and a second protein film, wherein the first protein film is a protein film after cross-linking treatment; attaching one surface of the first protein film to one surface of the second protein film to obtain an attached protein film, wherein a protein solution is applied between the attached surfaces of the first protein film and the second protein film in the attached protein film; And (3) carrying out crosslinking treatment on the attached protein film so that the protein solution connects the first protein film and the second protein film together through crosslinking reaction to obtain the composite protein film. In some alternative implementations, the second protein film is a cross-linked protein film, and the first protein film and the second protein film are both wet protein films, and the bonding of one surface of the first protein film to one surface of the second protein film to obtain a bonded protein film comprises: Applying a protein solution to one surface of the first protein film; And bonding one surface of the second protein film with the surface of the first protein film, to which the protein solution is applied, thereby obtaining the bonded protein film. In some alternative implementations, the first protein film is a wet protein film and the second protein film is a dry protein film that has not been cross-linked, and the bonding of one surface of the first protein film to one surface of the second protein film results in a bonded protein film comprising: and attaching one surface of the first protein film to one surface of the second protein film, so that the part of the surface of the second protein film attached to the first protein film is dissolved to form a protein solution, and the attached protein film is obtained. In some alternative implementations, the second protein film is a cross-linked protein film, and the first protein film and the second protein film are both dry protein films, and the bonding of one surface of the first protein film to one surface of the second protein film to obtain a bonded protein film comprises: Applying a protein solution to one surface of the first protein film; And bonding one surface of the second protein film with the surface of the first protein film, to which the protein solution is applied, thereby obtaining the bonded protein film. In some alternative implementations, the second protein film is a cross-linked protein film, and the first protein film and the second protein film are both dry protein films, and the bonding of one surface of the first protein film to one surface of the second protein film to obtain a bonded protein film comprises: Plasma treating one surface of the first protein film and one surface of the second protein film respectively; applying a protein solution to the plasma-treated surface of the first protein film; And (3) attaching the surface of the second protein film subjected to plasma treatment to the surface of the first protein film to which the protein solution is applied, so as to obtain the attached protein film. In some alternative implementations, the proteins in the protein solution, the first protein film forming protein, and the second protein film forming protein are the same species. In some alternative implementations, the protein in the protein solution is fibroin. In some alternative implementations, the conformable protein film is cross-linked such that the protein solution joins the first protein film and the second protein film together by