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CN-117447621-B - Oligosaccharide, preparation method thereof and application thereof in aquatic feed

CN117447621BCN 117447621 BCN117447621 BCN 117447621BCN-117447621-B

Abstract

The invention provides oligosaccharide, a preparation method thereof and application thereof in aquatic feed, and belongs to the technical field of plant polysaccharide. The invention sequentially carries out pectase enzymolysis, H 2 O 2 -Vc degradation and microwave degradation on mulberry polysaccharide, and the obtained degradation product is subjected to sulfation modification to obtain sulfated mulberry oligosaccharide. The sulfated mulberry oligosaccharide has the advantages of improving the oxidation resistance of aquatic animals, improving the antiviral property of the aquatic animals, improving the immunity of the aquatic animals and improving the growth performance of the aquatic animals in aquaculture, and can be used as a feed additive in aquaculture.

Inventors

  • LI ERNA
  • ZHOU DONGLAI
  • YANG QIONG
  • ZHANG WEILONG
  • LIAO SENTAI
  • LI QINGRONG
  • ZOU YUXIAO

Assignees

  • 广东省农业科学院蚕业与农产品加工研究所

Dates

Publication Date
20260512
Application Date
20231025

Claims (7)

  1. 1. Use of sulfated mulberry oligosaccharide in the preparation of at least one of the following: 1) improving the oxidation resistance of aquatic animals, 2) improving the antiviral property of the aquatic animals, and 3) improving the immunity of the aquatic animals; The aquatic animal is micropterus salmoides; The virus is iridovirus; the preparation method of the sulfated mulberry oligosaccharide comprises the following steps: Sequentially carrying out pectase enzymolysis, H 2 O 2 -Vc degradation and microwave degradation on mulberry polysaccharide to obtain degradation products; And (3) carrying out sulfation modification on the degradation product to obtain the sulfated mulberry oligosaccharide.
  2. 2. The use according to claim 1, wherein the final concentration of pectase is 5-10 g/L when the pectase is enzymatically hydrolyzed.
  3. 3. The use according to claim 1, wherein the temperature of the pectase is 40-60 ℃, and the time of the pectase is 25-35 min.
  4. 4. The use according to claim 1, wherein the final concentration of H 2 O 2 is 1-5% and the final concentration of Vc is 10-15% when the H 2 O 2 -Vc is degraded.
  5. 5. The application of claim 1, wherein the degradation temperature of H 2 O 2 -Vc is 48-52 ℃, and the degradation time of H 2 O 2 -Vc is 8-12 min.
  6. 6. The use according to claim 1, wherein the microwave power is 600-800 w and the microwave treatment time is 8-12 min.
  7. 7. The use according to claim 1, characterized in that the sulfation modification is performed by water-bath reaction of the degradation product and sulfur trioxide-pyridine; the final concentration of the sulfur trioxide-pyridine is 2% -5%, the temperature of the water bath reaction is 78-82 ℃, and the time of the water bath reaction is 1-1.5 h.

Description

Oligosaccharide, preparation method thereof and application thereof in aquatic feed Technical Field The invention belongs to the technical field of plant polysaccharide, and particularly relates to oligosaccharide, a preparation method thereof and application thereof in aquatic feed. Background The mulberry is a common fruit, has slightly sweet and delicious fruits and extremely high nutritive value, and contains various nutrient components such as rich vitamins, minerals, mulberry polysaccharide, amino acids and the like. Wherein mulberry polysaccharide has various beneficial effects and actions on human health. Mulberry polysaccharide is reported to have hypolipidemic effects, lower cholesterol and triacylglycerol levels in the blood, and reduce the risk of atherosclerosis. Mulberry polysaccharide has the effect of stimulating the immune system of the organism and has the anti-tumor effect. Meanwhile, mulberry polysaccharide has strong immunity enhancing effect and disease resistance enhancing effect. The mulberry polysaccharide also has strong antioxidation, can remove free radicals, and has positive significance for preventing and treating various degenerative diseases. In addition, mulberry polysaccharide has the functions of protecting liver and promoting gastrointestinal health, and has various benefits for maintaining human health. The mulberry oligosaccharide is a sugar structure with smaller molecular weight, which is obtained by further degrading mulberry polysaccharide as a raw material, and the prior art reports that the mulberry oligosaccharide is prepared into a prebiotic product for keeping human health. However, there is no report on the use of sulfated mulberry oligosaccharides for antiviral purposes. Disclosure of Invention In view of the above, the present invention aims to provide a method for preparing sulfated mulberry oligosaccharide, which has antiviral activity by multi-step degradation and sulfation modification of protease, H 2O2 -Vc and microwaves. The invention provides a preparation method of sulfated mulberry oligosaccharide, which comprises the following steps: Sequentially carrying out pectase enzymolysis, H 2O2 -Vc degradation and microwave degradation on mulberry polysaccharide to obtain degradation products; And (3) carrying out sulfation modification on the degradation product to obtain the sulfated mulberry oligosaccharide. Preferably, the final concentration of the pectase is 5-10 g/L during enzymolysis of the pectase. Preferably, the enzymolysis temperature of the pectase is 40-60 ℃, and the enzymolysis time of the pectase is 25-35 min. Preferably, when the H 2O2 -Vc is degraded, the final concentration of the H 2O2 is 1% -5%, and the final concentration of the Vc is 10% -15%. Preferably, the degradation temperature of the H 2O2 -Vc is 48-52 ℃, and the degradation time of the H 2O2 -Vc is 8-12 min. Preferably, when the microwaves are degraded, the power of the microwaves is 600-800W, and the treatment time of the microwaves is 8-12 min. Preferably, the sulfation modification method is to subject the degradation product and sulfur trioxide-pyridine to a water bath reaction; the final concentration of the sulfur trioxide-pyridine is 2% -5%, the temperature of the water bath reaction is 78-82 ℃, and the time of the water bath reaction is 1-1.5 h. The invention provides sulfated mulberry oligosaccharide prepared by the preparation method, the molecular weight of the sulfated mulberry oligosaccharide is 3000 Da-5000 Da, the sulfated mulberry oligosaccharide comprises glucose, xylose and galactose, the molar ratio of the glucose to the xylose to the galactose is 1 (1-2) (6-8), the hydroxyl of the monosaccharide is replaced by sulfonic acid groups in the sulfated mulberry oligosaccharide, and the substitution degree of the polysaccharide of the sulfonic acid groups is 1.251. The invention provides an animal feed, comprising the sulfated mulberry oligosaccharide and a basic feed. The invention provides application of the sulfated mulberry oligosaccharide in aquaculture, wherein the application comprises at least one of the following matters: 1) Improving the antioxidation capability of aquatic animals; 2) Improving the antiviral property of aquatic animals; 3) Improving the immunity of aquatic animals; 4) Improving the growth performance of aquatic animals. The invention provides a preparation method of sulfated mulberry oligosaccharide, which comprises the following steps of sequentially carrying out pectase enzymolysis, H 2O2 -Vc degradation and microwave degradation on mulberry polysaccharide to obtain degradation products, and carrying out sulfation modification on the degradation products to obtain the sulfated mulberry oligosaccharide. The sulfated mulberry oligosaccharide prepared by the invention not only has antiviral activity, but also has antioxidant capacity, improves the immunity of fed animals and promotes the growth of animals, and can be used as a feed additi