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CN-117511789-B - Brevibacillus laterosporus and application thereof

CN117511789BCN 117511789 BCN117511789 BCN 117511789BCN-117511789-B

Abstract

The invention relates to the technical field of microorganisms, in particular to Brevibacillus laterosporus and application thereof. The Brevibacillus laterosporus (Brevibacillus laterosporus) HNBR05-1 provided by the invention is preserved in China general microbiological culture Collection center (CGMCC) with the preservation number of CGMCC No. 25886. The Brevibacillus laterosporus HNBR05-1 has the function of efficiently inhibiting plant pathogenic bacteria, has the function of controlling plant diseases such as brown spot perforin disease, bacterial perforin disease and the like, has high control effect, has good application prospect in plant disease control, and lays a foundation for developing efficient biocontrol agents.

Inventors

  • BI QIUYAN
  • MA AIHONG
  • ZHANG HE
  • CHEN ZHENG
  • WANG DUO

Assignees

  • 河北省农林科学院植物保护研究所

Dates

Publication Date
20260505
Application Date
20231103

Claims (7)

  1. 1. The Brevibacillus laterosporus (Brevibacillus laterosporus) HNBR05-1 strain is characterized in that the HNBR05-1 strain is preserved in China general microbiological culture collection center (CGMCC) No. 25886.
  2. 2. A microbial preparation comprising the HNBR05-1 strain of claim 1.
  3. 3. A biocontrol formulation comprising the HNBR05-1 strain of claim 1 or prepared from the HNBR05-1 strain of claim 1.
  4. 4. The method for preparing a microbial preparation according to claim 2 or a biocontrol preparation according to claim 3, characterized in that the method comprises the step of culturing the HNBR05-1 strain under 33-37 ° C, pH 6.3.3-7.5 and aeration conditions to obtain a living bacterial culture; the culture medium used for the culture has the following composition of sucrose 15 g/L,K 2 HPO 4 4.8 g/L,KH 2 PO 4 3.5 g/L,(NH 4 )2SO 4 2 g/L,MgCl 2 0.16 g/L,CaCl 2 0.02 g/L,Na 2 MoO 4 •2H 2 O 0.0024 g/L,FeCl 3 0.0018 g/L,MnCl 2 •2H 2 O 0.0015 g/L, sodium chloride 2 g/L.
  5. 5. Use of HNBR05-1 strain according to claim 1 or a microbial preparation according to claim 2 or a biocontrol agent according to claim 3 for the inhibition of phytopathogens; Wherein the pathogenic bacteria are xanthomonas campestris Prunus persica variety (Xanthomonas arboricola pv. prune) Vauserin, hoste, kersters & Swings) and/or cercospora nucleotida (Cercospora circumscissa); the plant is peach.
  6. 6. Use of HNBR05-1 strain of claim 1 or the microbial preparation of claim 2 or the biocontrol agent of claim 3 in plant disease control; Wherein the disease is bacterial perforin disease and/or brown spot perforin disease; the plant is peach.
  7. 7. A method for controlling plant diseases, which comprises applying the HNBR05-1 strain of claim 1 or the microbial preparation of claim 2 or the biocontrol agent of claim 3 to said plant; Wherein the disease is bacterial perforin disease and/or brown spot perforin disease; the plant is peach.

Description

Brevibacillus laterosporus and application thereof Technical Field The invention relates to the technical field of microorganisms, in particular to Brevibacillus laterosporus and application thereof. Background The fruit tree planting process is relatively long, the risk of occurrence of diseases is high, and serious loss is easily caused if the disease prevention and control measures are not good. However, although the traditional chemical agents are used for disease control and possibly have better effects, harmful substances are liable to remain, so that the quality and safety of fruits are reduced. With the progress of technology, biological control means become the means of the most preferred consideration in fruit tree disease control, and play an important role in fruit tree disease control. In the cultivation management of fruit tree planting, the perforation disease of fruit trees is very common. The perforation disease causes a large amount of fallen leaves of the fruit trees, seriously causes the dead branch tips, reduces the yield, can influence the formation of flower buds, and various stone fruit types such as peach, plum, apricot, cherry and the like occur. Peach trees are one of the oldest fruit trees in China, and the perforation disease is most obvious on peach trees. In many areas, the flowers are withered in five six months each year, which is the full-growing period of the perforation disease of the peach tree, the perforation disease is easier to occur in the seasons with warm and rainy days each year, a great amount of leaves of the peach tree can fall off too early and die after the perforation disease occurs, and the problem of spot cracking on the surface of the fruit can be caused, so that the prevention and the control are very difficult, and the damage to the yield and the fruit quality of the peach tree are very large. The common peach perforation disease at present is bacterial perforation disease and brown spot perforation disease, and single and mixed occurrence phenomenon exists. Although the disease is perforation disease, different types of perforation disease have different disease characteristics and prevention methods, many fruit farmers cannot accurately judge the type of perforation disease after the occurrence of the disease, and blind medication often delays prevention and treatment, so that the perforation disease of peach trees is aggravated. The antibacterial spectrum, the prevention object, the prevention effect and the lasting effect of different biocontrol bacteria are different, so that when the peach tree perforation disease is prevented, the simultaneous treatment medicine for preventing and treating the bacterial perforation disease and the brown spot perforation disease is specifically found, and the optimal prevention effect of the disease can be achieved. Bacterial perforins of peaches are world-wide epidemic diseases with high incidence and serious hazard degree in recent years, and severely restrict the economic development of peach industry. The etiology of bacterial perforin disease in peach is Xanthomonas campestris Prunus persica variety (Xanthomonas carboricolapv. Prune) Vauserin, hoste, kersters & Swings. Once the disease occurs, the disease is extremely easy to be influenced by high-temperature and high-humidity weather and has large area burst, is difficult to control, and can only be forced to cut down the diseased tree to prevent the infectious agent. The scientific management and prevention and control of the disease must be considered to be important. The chemical agents including copper preparations are used in a large quantity, the effect is obvious and quick, but the problems of environmental pollution, pesticide residues, drug resistance and the like are outstanding, and a new green, environment-friendly and effective prevention and control alternative way, namely biological prevention and control, is urgently sought. However, the biological control technology for bacterial perforins of peach is not mature at present, and only a few reports are made on Esitken et al （Effects of foliar application of Bacillus subtilis OSU-142 on the yield, growth and control of shot-hole disease (Coryneum blight) of apricot. European Journal of Horticultural Science, 2002, 67(4).S. 139-142） and Biondi et al （Use of a bacterial antagonist for the biological control of bacterial leaf/fruit spot of stone fruits. Iobc/wprs Bulletin, 2009, 277-281） that partial biocontrol bacteria bacillus subtilis and pseudomonas fluorescens can control bacterial perforins of stone fruits. Kawaguchi et al （Biological control of bacterial spot on peach by nonpathogenicXanthomonas campestrisstrains AZ98101 and AZ98106. Journal of General Plant Pathology, 2014, 80:158-163） sprayed suspension of nonpathogenic Xanthomonas strains AZ98101 and AZ98106 on peach trees, followed by Xap inoculation, and the results showed that both strains could inhibit the proliferation of bacterial