CN-117531243-B - Automatic high performance liquid chromatographic column regeneration device and use method thereof

CN117531243BCN 117531243 BCN117531243 BCN 117531243BCN-117531243-B

Abstract

The invention discloses an automatic high-efficiency liquid chromatographic column regeneration device and a use method thereof, wherein the automatic high-efficiency liquid chromatographic column regeneration device comprises a column temperature cabin and a power cabin, a temperature control module is arranged in the column temperature cabin, a chromatographic column is installed in the temperature control module, a switching valve A, a switching valve B, a switching valve C, a switching valve D and an infusion pump are arranged in the power cabin, the switching valve A, the switching valve B and the switching valve C are multi-way selector valves, the switching valve D is a two-position four-way switching valve, a plurality of branch interfaces of the switching valve A are externally connected with a plurality of solvent bottles through pipelines, a common interface of the switching valve A is connected with an infusion pump, four interfaces of the switching valve D are respectively connected with the infusion pump, the switching valve B, the common interface and the waste liquid bottle, a plurality of branch interfaces of the switching valve B and the switching valve C are respectively connected with two ends of a plurality of chromatographic columns, and the flushing solvent, the flushing direction and the chromatographic column to be regenerated can be automatically switched through four switching.

Inventors

HU YI
TIAN NA
BU ZHENHUA
WANG HEWEI
ZHANG HONGSHENG
ZHANG YUANLI
TANG YUANYUAN

Assignees

诺米(大连)机器人智能科技有限公司

Dates

Publication Date: 20260505
Application Date: 20231110

Claims (8)

1. The automatic high-efficiency liquid chromatographic column regeneration device is characterized by comprising a column temperature cabin and a power cabin, wherein a temperature control module is arranged in the column temperature cabin, a plurality of chromatographic columns are arranged on the temperature control module, a switching valve A, a switching valve B, a switching valve C, a switching valve D and an infusion pump are arranged in the power cabin, the switching valve A, the switching valve B and the switching valve C are multi-way selector valves and are all provided with a public interface and a plurality of branch interfaces, the switching valve D is a two-position four-way selector valve, and a port 1, a port 2, a port 3 and a port 4 are arranged; The liquid inlet of the infusion pump is connected with the liquid outlet of the infusion pump, the liquid outlet of the infusion pump is connected with the No. 1 port of the switching valve D, the common interface of the switching valve B is connected with the No. 2 port of the switching valve D, the plurality of branch interfaces of the switching valve B are respectively connected with one ends of a plurality of chromatographic columns, the other ends of the plurality of chromatographic columns are respectively connected with the plurality of branch interfaces of the switching valve C, the common interface of the switching valve C is connected with the No. 4 port of the switching valve D, and the No.3 port of the switching valve D is externally connected with a waste liquid bottle or a chromatographic detector through a pipeline; The device comprises a switching valve C, a plurality of branch interfaces of the switching valve B, a flushing solvent and a reverse flushing, wherein the branch interfaces of the switching valve C are respectively connected with the top ends of a plurality of chromatographic columns, the branch interfaces of the switching valve B are respectively connected with the bottom ends of a plurality of chromatographic columns, the flushing solvent flows in from the top ends of the chromatographic columns through the switching valve C and flows out from the bottom ends of the chromatographic columns to the switching valve B for forward flushing, and reverse flushing is performed, the port 2 and the port 4 of the switching valve D are arranged in center symmetry and are adjacent to the port 1 and the port 3, the switching valve D is controlled to enable the port 1 to be communicated with the port 4, the port 2 to be communicated with the port 3 to realize forward flushing, or the port 1 to be communicated with the port 2, and the port 3 to be communicated with the port 4 to realize reverse flushing.
2. The automatic high performance liquid chromatography column regeneration device according to claim 1, further comprising a box body, wherein the column temperature cabin and the power cabin are arranged in the box body side by side, a plurality of through holes are formed in one side wall of the power cabin, a pipeline connected with a branch interface of the switching valve A penetrates through the through holes to be connected with a solvent bottle outside the box body, and a pipeline connected with a port 3 of the switching valve D penetrates through the through holes to be connected with a waste liquid bottle or a chromatography detector outside the box body.
3. The automatic high performance liquid chromatography column regeneration device according to claim 2, wherein the switching valve A and the switching valve B are arranged above the infusion pump side by side, the switching valve C and the switching valve D are arranged above the switching valve A and the switching valve B side by side, and the switching valve B and the switching valve C are arranged near one of the column temperature cabins.
4. An automatic high performance liquid chromatography column regeneration device according to claim 3, wherein a partition board and a main frame are arranged in the box body, the partition board divides the interior of the box body into a Cheng Zhuwen cabin and a power cabin, the main frame is positioned at the rear side of the partition board, and the temperature control module, the switching valve A, the switching valve B, the switching valve C, the switching valve D and the infusion pump are positioned on the main frame.
5. The apparatus of claim 2, wherein the bottoms of the column temperature chamber and the power chamber are respectively provided with a liquid leakage groove, the liquid leakage grooves are provided with liquid leakage sensors, and the bottoms of the side walls of the box body are provided with waste discharge ports communicated with the liquid leakage grooves.
6. The apparatus of claim 2, wherein a door of the switch is provided on a front side of the case, and a control panel and keys are provided on the door.
7. The apparatus of claim 1, wherein the valve heads of the switching valve A, the switching valve B, the switching valve C and the switching valve D and the pump head of the infusion pump are made of SUS, PEEK or Teflon.
8. The method for using the automatic high performance liquid chromatography column regeneration device according to claim 1, which is characterized by comprising the following steps: step 1, installing a chromatographic column on a temperature control module, and starting a column temperature chamber to set temperature; Step 2, controlling a switching valve B and a switching valve C to select chromatographic columns needing regeneration; step 3, controlling a switching valve A to select a flushing solvent; step4, controlling a switching valve D to select forward flushing or backward flushing; Step 5, setting the flow rate and the flushing time of the infusion pump, and starting the infusion pump to flush; step 6, after the flushing is finished, controlling a switching valve A to switch the flushing solvent, and repeating the steps 4-5; And 7, after washing is completed, controlling the switching valve B and the switching valve C to switch the next chromatographic column to be washed, and repeating the steps 3-6 to complete washing of all chromatographic columns.

Description

Automatic high performance liquid chromatographic column regeneration device and use method thereof Technical Field The invention belongs to the technical field of chromatographic column regeneration, and particularly relates to an automatic high-performance liquid chromatographic column regeneration device and a use method thereof. Background High performance liquid chromatography is a novel analytical separation technology developed in the beginning of the last 70 years of 60. The method is widely applied to the fields of biochemistry, medicine analysis and production, environment detection, chemistry and chemical industry, food sanitation, commodity detection and the like by the characteristics of high separation efficiency, high analysis speed, good detection sensitivity and the like. The core of the high performance liquid chromatograph is a chromatographic column, and the performance of the chromatographic column is directly related to the accuracy of measurement. The chromatographic column can cause the reduction of column efficiency and even complete failure after long-time use or under the condition of improper application, and the regeneration treatment of the chromatographic column with poor performance is carried out, so that the performance is recovered as much as possible, thereby being an economic and effective way. A typical method of regenerating a chromatographic column is to place the column in a chromatograph for washing or to wash the column with a column washing device. Flushing with chromatograph can occupy chromatograph detection position, reduce instrument availability factor, and current chromatographic column flushing device has following not enough: 1. in the flushing process, a plurality of solvents are often needed, the existing chromatographic column flushing device can only install one chromatographic column and one flushing solvent, the solvents are required to be frequently switched, the solvents are required to be manually switched, the operation is complex, and the accuracy is not easy to control; 2. The washing process often needs forward washing and then back washing, then forward washing, forward back washing alternately, and current chromatographic column washing unit generally only has a washing direction, and the switching washing direction needs to pull down the chromatographic column upset and install again, also needs manual switching, and the operation is complicated, and the human cost is high, and economic benefits is low. Disclosure of Invention The invention aims to provide an automatic high-efficiency liquid chromatographic column regenerating device and a use method thereof, which can automatically switch flushing solvent, flow direction and flow velocity, and solve the problems that the existing chromatographic column flushing device needs manual operation, and has complex operation, high labor cost and low economic benefit. In order to achieve the aim, the invention provides an automatic high performance liquid chromatography column regeneration device which comprises a column temperature cabin and a power cabin, wherein a temperature control module is arranged in the column temperature cabin, a plurality of chromatography columns are arranged on the temperature control module, a switching valve A, a switching valve B, a switching valve C, a switching valve D and an infusion pump are arranged in the power cabin, the switching valve A, the switching valve B and the switching valve C are multi-way selector valves, a common interface and a plurality of branch interfaces are respectively arranged, the switching valve D is a two-position four-way selector valve, and a port 1, a port 2, a port 3 and a port 4 are arranged; The liquid inlet of the infusion pump is connected with the liquid outlet of the infusion pump, the liquid outlet of the infusion pump is connected with the No. 1 port of the switching valve D, the common interface of the switching valve B is connected with the No. 2 port of the switching valve D, the plurality of branch interfaces of the switching valve B are respectively connected with one ends of a plurality of chromatographic columns, the other ends of the plurality of chromatographic columns are respectively connected with the plurality of branch interfaces of the switching valve C, the common interface of the switching valve C is connected with the No. 4 port of the switching valve D, and the No.3 port of the switching valve D is connected with a waste liquid bottle or a chromatographic detector through the pipeline. Further, the plurality of branch interfaces of the switching valve C are respectively connected with the top ends of the plurality of chromatographic columns, the plurality of branch interfaces of the switching valve B are respectively connected with the bottom ends of the plurality of chromatographic columns, and the flushing solvent flows in from the top ends of the chromatographic columns through the switching valve C an