CN-117547863-B - Chromatographic experimental device and experimental method

CN117547863BCN 117547863 BCN117547863 BCN 117547863BCN-117547863-B

Abstract

The invention relates to the technical field of experimental equipment, in particular to a chromatographic experimental device and an experimental method. During chromatography experiments, the first chromatographic column and the second chromatographic column can be in a serial state by controlling the first valve structure, the second valve structure and the third valve structure, so that liquid in the liquid inlet pipeline can sequentially pass through the first chromatographic column and the second chromatographic column. The first chromatographic column and the second bypass pipeline can be communicated, liquid in the liquid inlet pipeline sequentially passes through the first chromatographic column and the second bypass pipeline, the second chromatographic column and the first bypass pipeline are also communicated, and when the operation of alkali disinfection, pre-balancing, sample loading, balancing, regeneration, alkali disinfection, balancing and preserving is performed, the first chromatographic column and the second chromatographic column which are connected in series can be simultaneously processed, and compared with the operation of alkali disinfection, pre-balancing, sample loading, balancing, regeneration, alkali disinfection, balancing and preserving, which are separately performed on the first chromatographic column and the second chromatographic column, the experimental time is saved, and the experimental efficiency is improved.

Inventors

LI YAO
ZHOU XING
ZHAO WENJIE
HE LIU
LI XIAOFANG

Assignees

深圳市乐土生命科技投资有限公司

Dates

Publication Date: 20260512
Application Date: 20231226

Claims (9)

1. The chromatographic experiment device is characterized by comprising a liquid inlet pipeline, a first chromatographic column, a second chromatographic column, a first serial pipeline, a first bypass pipeline connected with the first chromatographic column in parallel and a second bypass pipeline connected with the second chromatographic column in parallel, wherein the first serial pipeline is used for connecting the first chromatographic column and the second chromatographic column in series; The two ends of the first bypass pipeline are respectively connected with the first valve structure and the second valve structure, and the two ends of the first chromatographic column are respectively connected with the first valve structure and the second valve structure; the first valve structure is connected with the liquid inlet pipeline and is used for enabling liquid in the liquid inlet pipeline to enter a first chromatographic column or a first bypass pipeline selectively, and the second valve structure is used for enabling liquid flowing out of the first chromatographic column or liquid flowing out of the first bypass pipeline to enter the first serial pipeline selectively; the third valve structure is positioned between the second valve structure and the second chromatographic column, and the liquid inlet end of the second chromatographic column is connected with the third valve structure; the liquid inlet end of the second bypass pipeline is connected with the third valve structure, and the third valve structure is used for enabling liquid of the first serial pipeline to selectively enter the second bypass pipeline or the second chromatographic column; The chromatographic test method comprises the following steps: The liquid in the liquid inlet pipeline sequentially passes through a first chromatographic column and a second chromatographic column which are connected in series by controlling a first valve structure, a second valve structure and a third valve structure, and the first chromatographic column and the second chromatographic column are subjected to alkali disinfection, pre-balancing and balancing operation before sample loading; The liquid in the liquid inlet pipeline sequentially passes through the first bypass pipeline and the second bypass pipeline through controlling the first valve structure, the second valve structure and the third valve structure, so that the first chromatographic column sequentially performs sample loading, sample balancing and elution operations; By controlling the first valve structure, the second valve structure and the third valve structure, the liquid in the liquid inlet pipeline sequentially passes through the first bypass pipeline and the second bypass pipeline, and the balance operation is carried out on the first bypass pipeline and the second bypass pipeline; By controlling the first valve structure, the second valve structure and the third valve structure, the liquid in the liquid inlet pipeline sequentially passes through the first chromatographic column and the second chromatographic column which are connected in series, and the first chromatographic column and the second chromatographic column are sequentially subjected to regeneration, alkali disinfection, balance and preservation operations.
2. The chromatography assay of claim 1, wherein the chromatography assay comprises a third chromatography column and a second series line for connecting the second chromatography column in series with the third chromatography column, the chromatography assay further comprising a third bypass line in parallel with the third chromatography column; The fifth valve structure is arranged between the fourth valve structure and the third chromatographic column, the liquid inlet end of the third chromatographic column is connected with the fifth valve structure, the liquid inlet end of the third bypass pipeline is connected with the fifth valve structure, the fourth valve structure is used for enabling liquid flowing out of the second chromatographic column or liquid flowing out of the second bypass pipeline to enter the second serial pipeline, and the fifth valve structure is used for enabling liquid of the second serial pipeline to enter the third bypass pipeline or the third chromatographic column.
3. The chromatography assay of claim 1, wherein the number of first chromatography columns is two or more, each of the first chromatography columns being arranged in parallel.
4. The chromatography assay of claim 2, wherein each of said first chromatography columns is connected to said first valve structure, said first valve structure enabling selective access of liquid from said liquid feed line to any number of said first chromatography columns.
5. A chromatography experimental method according to claim 3, wherein the first valve structure comprises a main valve and a branch valve, the main valve is a three-way valve, the branch valve is a stop valve, the branch valve corresponds to the first chromatography column one by one, the main valve is used for enabling the liquid of the liquid inlet pipeline to enter the first bypass pipeline or the branch valve selectively, and the branch valve is used for controlling the on-off between the first chromatography column corresponding to the branch valve and the main valve.
6. The chromatography assay of any one of claims 1-5, wherein at least one of the first valve structure, the second valve structure, and the third valve structure is a three-way valve.
7. The chromatography assay of claim 6 wherein the three-way valve is an electronically controlled valve.
8. The chromatography assay of claim 6, wherein the three-way valve is a manual valve.
9. The chromatography assay of any one of claims 1-5, wherein the number of feed lines is one.

Description

Chromatographic experimental device and experimental method Technical Field The invention relates to the technical field of experimental equipment, in particular to a chromatographic experimental device and an experimental method. Background The chromatographic process generally comprises the steps of alkali disinfection, pre-equilibration, pre-loading equilibration, loading, post-loading equilibration, elution, regeneration, alkali disinfection, equilibration, preservation and the like, and the steps are the same except for the operations of loading, post-equilibration, elution and the like. In the current chromatography experiments, a chromatography experiment device is generally provided with a liquid inlet pipeline, and each step is independently operated at one time. When more than two chromatographic columns are needed, each chromatographic column needs to be subjected to separate chromatographic operation in sequence, the experimental time is longer, and the chromatographic efficiency is low. Disclosure of Invention The invention provides a chromatographic experimental device and an experimental method, which are used for solving the technical problem of low chromatographic efficiency of the conventional chromatographic experimental device. In a first aspect, an embodiment provides a chromatographic experimental device, which comprises a liquid inlet pipeline, a first chromatographic column, a second chromatographic column, a first serial pipeline, a first bypass pipeline connected with the first chromatographic column in parallel and a second bypass pipeline connected with the second chromatographic column in parallel, wherein the first serial pipeline is used for connecting the first chromatographic column and the second chromatographic column in series; The two ends of the first bypass pipeline are respectively connected with the first valve structure and the second valve structure, and the two ends of the first chromatographic column are respectively connected with the first valve structure and the second valve structure; the first valve structure is connected with the liquid inlet pipeline and is used for enabling liquid in the liquid inlet pipeline to enter a first chromatographic column or a first bypass pipeline selectively, and the second valve structure is used for enabling liquid flowing out of the first chromatographic column or liquid flowing out of the first bypass pipeline to enter the first serial pipeline selectively; The third valve structure is arranged between the second valve structure and the second chromatographic column, the liquid inlet end of the second chromatographic column is connected with the third valve structure, two ends of the first serial pipeline are respectively connected with the second valve structure and the third valve structure, the liquid inlet end of the second bypass pipeline is connected with the third valve structure, and the third valve structure is used for enabling liquid of the first serial pipeline to selectively enter the second bypass pipeline or the second chromatographic column. Further, in one embodiment, the chromatographic test device comprises a third chromatographic column and a second series pipeline, wherein the second series pipeline is used for connecting the second chromatographic column with the third chromatographic column in series, and the chromatographic test device further comprises a third bypass pipeline connected with the third chromatographic column in parallel; The fifth valve structure is arranged between the fourth valve structure and the third chromatographic column, the liquid inlet end of the third chromatographic column is connected with the fifth valve structure, the liquid inlet end of the third bypass pipeline is connected with the fifth valve structure, the fourth valve structure is used for enabling liquid flowing out of the second chromatographic column or liquid flowing out of the second bypass pipeline to enter the second serial pipeline, and the fifth valve structure is used for enabling liquid of the second serial pipeline to enter the third bypass pipeline or the third chromatographic column. Further, in one embodiment, the number of the first chromatographic columns is more than two, and each of the first chromatographic columns is arranged in parallel. Further, in one embodiment, each of the first chromatographic columns is connected to a first valve structure that enables the liquid in the liquid inlet line to be selectively introduced into any number of the first chromatographic columns. Further, in an embodiment, the first valve structure includes a main valve and a branch valve, the main valve is a three-way valve, the branch valve is a stop valve, the branch valve corresponds to the first chromatographic column one by one, the main valve is used for enabling the liquid in the liquid inlet pipeline to selectively enter the first bypass pipeline or the branch valve, and the branch valve is used for con