CN-117547864-B - Liquid path bubble removing device and liquid path bubble removing method

CN117547864BCN 117547864 BCN117547864 BCN 117547864BCN-117547864-B

Abstract

The invention provides a liquid path bubble removing device and a liquid path bubble removing method, wherein the liquid path bubble removing device comprises a cleaning liquid barrel, a liquid tank and a liquid tank, wherein the cleaning liquid barrel is used for containing cleaning liquid; the cleaning device comprises a cleaning solution buffer tank, a switching switch and a pumping device, wherein the cleaning solution buffer tank is a closed tank body and is communicated with a cleaning solution barrel, the cleaning solution buffer tank is provided with an exhaust pipe and a liquid conveying pipe, and the pumping device is communicated with the exhaust pipe or the liquid conveying pipe through one of the switching switches. In the scheme, through generating negative pressure in the cleaning solution buffer tank, the negative pressure enables the cleaning in the cleaning solution barrel to continuously flow into the cleaning solution buffer tank, and bubbles in the cleaning solution are separated out under the action of the negative pressure and gathered at the upper part in the holding cavity of the cleaning solution buffer tank, and the bubbles are effectively removed from the cleaning solution at the lower part of the holding cavity, so that the accuracy of the sample adding quantity is ensured. The invention has the advantages of simple use, small occupied space, higher reliability, obvious bubble removal effect and lower manufacturing cost.

Inventors

MENG GANG
LIANG YANWEI
GAO CHUNHAI

Assignees

北京铂茵生物科技有限公司

Dates

Publication Date: 20260505
Application Date: 20230314
Priority Date: 20220715

Claims (9)

1. A liquid path bubble removal apparatus, comprising: A cleaning liquid barrel for containing cleaning liquid; The cleaning solution buffer tank is a closed tank body and is communicated with the cleaning solution barrel, and is provided with an exhaust pipe for absorbing gas in the cleaning solution buffer tank and a liquid feeding pipe for extracting cleaning solution in the cleaning solution buffer tank; The pumping device is selectively communicated with the exhaust pipe or the liquid conveying pipe through the change-over switch; the switching switch is a two-position three-way valve, wherein the normally closed port of the two-position three-way valve is communicated with the exhaust pipe; the public port of the two-position three-way valve is communicated with the pumping device; the pumping device comprises a diaphragm pump and a plunger pump communicated with the diaphragm pump, wherein, The diaphragm pump is communicated with a public port of the two-position three-way valve; The sampling needle is communicated with the plunger pump; The cleaning solution buffer tank also comprises a liquid level float switch arranged in the cleaning solution buffer tank, wherein the liquid level float switch is used for controlling the change-over switch to switch; when the liquid level float switch is conducted, the pumping device is communicated with the exhaust pipe through the change-over switch.
2. The liquid circuit bubble removal apparatus according to claim 1, wherein the liquid feed pipe is provided at a position on a bottom wall or a side wall of the cleaning liquid buffer tank near the bottom wall.
3. The liquid circuit bubble removal apparatus as claimed in claim 1, wherein said air extraction tube is provided at a position on a top wall or a side wall of said cleaning liquid buffer tank near said top wall.
4. The liquid path bubble removal apparatus of claim 1, wherein, when the sampling needle is included, The needle cleaning device also comprises a needle cleaning pool positioned below the sampling needle.
5. The liquid path bubble removal apparatus according to claim 4, further comprising a waste liquid tank in communication with the needle wash tank.
6. The liquid circuit bubble removal apparatus of claim 1, wherein said liquid level float switch is suspended inside a top wall of said cleaning liquid buffer tank.
7. A method for bubble removal in a liquid path, comprising the steps of: Discharging the gas in the cleaning liquid buffer tank and filling the tank with cleaning liquid; filling a liquid conveying pipe between the cleaning liquid buffer tank and the sampling needle with cleaning liquid; pumping the cleaning liquid from the cleaning liquid buffer tank to the injector and the sampling needle through the pumping device, generating negative pressure in the cleaning liquid buffer tank through the pumping device, enabling the cleaning liquid in the cleaning liquid barrel to continuously flow into the cleaning liquid buffer tank through the negative pressure, and separating out bubbles in the cleaning liquid under the action of the negative pressure; and when the cleaning liquid in the cleaning liquid buffer tank is lower than the liquid level float switch, the liquid level float switch controls the change-over switch to switch, the exhaust pipe is communicated with the pumping device, and negative pressure is pumped to the cleaning liquid buffer tank.
8. The method of removing bubbles from a liquid path according to claim 7, wherein the pumping means is connected to the suction pipe by a change-over switch, and the air in the cleaning liquid buffer tank is discharged and filled with the cleaning liquid.
9. The method of removing bubbles from a liquid path according to claim 7, wherein filling the liquid supply pipe between the liquid buffer tank and the sampling needle with the liquid specifically comprises: The pumping device is communicated with the liquid conveying pipe through the change-over switch, and the liquid conveying pipe is filled with cleaning liquid through the pumping device.

Description

Liquid path bubble removing device and liquid path bubble removing method Technical Field The invention relates to the technical field of analytical instruments, in particular to a liquid path bubble removing device and a liquid path bubble removing method. Background In a sample analyzer, particularly in a chemiluminescent immunoassay analyzer, in order to ensure the accuracy of a detection result, the accuracy of a sample loading amount needs to be ensured in the process of loading a sample by an instrument. However, bubbles are often formed in the mobile phase solution in the liquid path system of the instrument due to dissolved oxygen or mixed air, and the bubbles affect the accuracy of the sample loading amount of the instrument. In the prior art, in order to remove bubbles in a liquid path system, a de-gassing membrane device which is already produced is often adopted in the liquid path system, and the basic principle is that a large number of hollow fibers are arranged in the de-gassing membrane, tiny holes are formed in the walls of the fibers, water molecules cannot pass through the small holes, and gas molecules can pass through the small holes. When the device works, water flow passes through the inside of the hollow fiber under a certain pressure, the air is continuously pumped out from the outside of the hollow fiber under the action of the vacuum pump, and a certain negative pressure is formed, so that the air in the water continuously overflows outwards from the water through the hollow fiber, and the purpose of removing the air in the water is achieved. The main disadvantage of the degassing device is that the cost is too high, and the degassing device occupies a large space inside the device. The current basic process of removing bubbles in a liquid path is to empty liquid in the liquid path, then stand for a certain time, wait for residual bubbles in the liquid path to break into a water film, and then flush the liquid without bubbles in the liquid path. The technology has the defects that standing is needed in the bubble removing process, the waiting time is long, the efficiency is low, and in addition, if bubbles exist in the liquid stored in the container, the method can not remove the bubbles in the liquid, and the technology has ideal use condition and low reliability. Disclosure of Invention Accordingly, it is an object of one or more embodiments of the present invention to provide a method for removing bubbles from a liquid path of a bubble removing device for improving the bubble removing effect of a cleaning liquid. In a first aspect, there is provided a liquid path bubble removal apparatus comprising: A cleaning liquid barrel for containing cleaning liquid; The cleaning solution buffer tank is a closed tank body and is communicated with the cleaning solution barrel, and is provided with an exhaust pipe for absorbing gas in the cleaning solution buffer tank and a liquid feeding pipe for extracting cleaning solution in the cleaning solution buffer tank; The pumping device is selectively communicated with the air extraction pipe or the liquid conveying pipe through the change-over switch. In the scheme, through generating negative pressure in the cleaning solution buffer tank, the negative pressure enables the cleaning in the cleaning solution barrel to continuously flow into the cleaning solution buffer tank, and bubbles in the cleaning solution are separated out under the action of the negative pressure and gathered at the upper part in the holding cavity of the cleaning solution buffer tank, and the bubbles are effectively removed from the cleaning solution at the lower part of the holding cavity, so that the accuracy of the sample adding quantity is ensured. The invention has the advantages of simple use, small occupied space, higher reliability, obvious bubble removal effect and lower manufacturing cost. In a specific implementation, the change-over switch is a two-position three-way valve, wherein the normally closed port of the two-position three-way valve is communicated with the exhaust pipe, and the normally open port of the two-position three-way valve is communicated with the liquid feeding pipe; the common port of the two-position three-way valve is communicated with the pumping device. In a specific embodiment, the pumping means comprises a diaphragm pump and a plunger pump in communication with the diaphragm pump, wherein, The diaphragm pump is communicated with a public port of the two-position three-way valve. In a specific embodiment, a sampling needle is also included, the sampling needle in communication with the plunger pump. In a specific implementation, the cleaning solution buffer tank further comprises a liquid level float switch arranged in the cleaning solution buffer tank, wherein the liquid level float switch is used for controlling the change-over switch to switch; when the liquid level float switch is conducted, the pumping device is communicated with