CN-117554351-B - Raman detection system, detection method and application thereof

Abstract

The invention discloses a Raman detection system, a detection method and application thereof, wherein the system comprises a Raman spectrometer and a fixed shading frame, one end of the fixed shading frame is fixedly connected with the Raman spectrometer, the other end of the fixed shading frame is provided with an entering groove for an needle-shaped SERS probe to enter, the tail end of the entering groove is provided with a slot for fixing the needle-shaped SERS probe, and the shape and the size of the slot are matched with those of the needle-shaped SERS probe. The Raman detection system provided by the invention is provided with the fixed shading device with the deep-colored groove and the focal length design, the groove can be used for limiting the rolling of the needle-shaped SERS probe curved surface structure, the deep color has a better shading effect, the laser can be more accurately focused on the curved surface by regulating and controlling the distance from the instrument probe to the SERS probe, the structure is compact and portable, the user operation is facilitated, the effective supplement to the SERS detection field is realized, the SERS technology can be expanded, and the application range is wider.

Inventors

• LV JIAN
• ZHOU BINBIN
• SHEN JUNDA
• LI ZEBIAO
• LI YANGYANG

Assignees

• 香港城市大学深圳福田研究院

Dates

Publication Date

20260512

Application Date

20220803

Claims (10)

1. 1. The Raman detection system is characterized by comprising a Raman spectrometer (1) and a fixed shading frame (2), wherein one end of the fixed shading frame (2) is fixedly connected with the Raman spectrometer (1), an entering groove (3) for an needle-shaped SERS probe (21) to enter is formed in the other end of the fixed shading frame, a slot (4) for placing and fixing the needle-shaped SERS probe (21) is formed in the tail end of the entering groove (3), and the shape and the size of the slot (4) are matched with those of the needle-shaped SERS probe (21).
2. 2. A raman detection system according to claim 1, characterized in that said entrance slot (3) is an elongated deep slot structure cut and extending from the side of the fixed mask (2) towards the center.
3. 3. Raman detection system according to claim 1, characterized in that said slot (4) is formed at the end of said entry slot (3) and extends along the end to one side.
4. 4. A raman detection system according to claim 3, characterized in that said slot (4) is perpendicular to the direction of extension of said entrance slot (3) forming a U-shaped groove structure, and that said slot (4) and said entrance slot (3) form an L-shape.
5. 5. A raman detection system according to claim 1, characterized in that said fixed mask (2) is provided at one end with a mounting hole (5), said mounting hole (5) being shaped and sized to correspond to the probe size of said raman spectrometer (1) for receiving and fixing the probe.
6. 6. A raman detection system according to claim 5, characterized in that an internal measuring platform is arranged in the mounting hole (5), the internal measuring platform is formed by protruding from the periphery of the inner wall of the mounting hole (5) to the central position, and is at a certain distance from the upper port of the mounting hole (5), and the cross-sectional size of a cavity below the internal measuring platform is smaller than the cross-sectional size of a cavity above the internal measuring platform, so as to fix the position of the probe in the vertical direction.
7. 7. A raman detection system according to claim 6 wherein the distance between the upper end surface of said internal measurement platform and said slot (4) is L, 1mm L100 mm.
8. 8. A raman detection system according to claim 1, characterized in that a latch type fixing structure (7) is formed at one end of the fixed shade frame (2), the latch type fixing structure (7) is provided with a semicircular groove (71) located at a middle position, two fixing surfaces (72) are formed by extending from an opening end to two sides in a diameter direction, two limiting blocks (73) are formed by extending ends of the two fixing surfaces (72) in a vertical direction at two sides below the semicircular groove (71), arc-shaped clamping grooves (74) are formed at opposite inner sides of the two limiting blocks (73), and a cavity for accommodating a probe of the raman spectrometer (1) is formed by the semicircular groove (71) and the two opposite arc-shaped clamping grooves (74).
9. 9. Detection method based on a raman detection system according to any one of claims 1 to 8, characterized in that it comprises the steps of: 1) Inserting a probe of a Raman spectrometer into a mounting hole or a semicircular groove of the fixed shading frame; 2) Inserting a needle-shaped SERS probe into a sample to be detected; 3) Pulling out the needle SERS probe and inserting the needle SERS probe into a slot of the fixed shading frame; 4) And (5) utilizing a Raman spectrometer to perform spectrum acquisition.
10. 10. Use of a raman detection system according to any one of claims 1 to 8 in the field of needle-like SERS probe detection.

Description

Raman detection system, detection method and application thereof Technical Field The invention belongs to the technical field of detection, relates to a Raman detection system, a detection method and application thereof, and in particular relates to a high-performance portable Raman detection system, a detection method and application thereof. Background In recent years, due to the characteristics of convenient raman spectrum operation, non-invasive test and the like, the method is widely applied to various fields such as food safety, pharmaceutical engineering, customs inspection and the like, and is the most potential Point of CARE TESTING, POCT. However, the portable raman instrument has low sensitivity, and is difficult to use for detecting trace substances in a complex system, so that the wider application of the raman technology is limited. Researchers find that noble metals with nanoscale roughened surfaces (referred to as "SERS probes") can enhance raman spectrum signals by a factor of one million, greatly promoting the application of portable raman instruments in various fields. The most critical issue at present is how to match a high-performance SERS probe with a portable raman instrument to form a high-performance portable raman detection system. Most of the existing SERS probes are plane substrates modified by noble metal nano particles, and the type of probes generally needs to use glass, silicon chips, aluminum foils and the like as substrates, and detect objects to be detected by dripping the objects to be detected on the surfaces of the substrates. The planar substrate has a plurality of problems in actual detection that (1) information in a block sample, such as a series of meat products including fish meat, beef and the like, cannot be obtained, (2) background interference signals in an object to be detected are also adsorbed on the surface of a chip together with a target object in a dripping mode to interfere with final detection, and (3) the planar substrate also has signals, such as a most commonly used silicon wafer has obvious Raman peaks at about 520 cm -1, and the interference can be brought to detection. Based on the above problems, a needle-like SERS probe is developed to be directly inserted into a bulk article for detection, and selectively adsorb target molecules to eliminate interference of background signals. However, all the fixing devices related to SERS (surface enhanced raman scattering) detection procedures on the market are developed based on planar SERS probes, and these fixing devices are usually designed according to the dimensions and the height of the planar SERS probes, and cannot meet the requirements of needle-shaped SERS probes. Therefore, there is a need to develop a raman detection system for needle-like SERS probe immobilization and detection procedures. Disclosure of Invention The invention aims to provide a Raman detection system, a detection method and application thereof, which are applied to a detection flow of an acicular SERS probe and have excellent detection performance. In order to achieve the above object, according to one aspect of the present invention, there is provided a raman detection system comprising a raman spectrometer and a fixed mask having one end connected and fixed to the raman spectrometer and the other end provided with an entry slot for an needle-shaped Surface Enhanced Raman Scattering (SERS) probe to enter, and a slot for fixing the needle-shaped SERS probe is formed at the end of the entry slot, and the slot has a shape and size adapted to the needle-shaped SERS probe. According to one embodiment of the invention, the system is a portable Raman detection system with excellent performance. According to one embodiment of the invention, the entrance groove is an elongated deep groove structure formed by cutting and extending from the side surface of the fixed shading frame to the center. According to one embodiment of the invention, the slot is formed at the end of the access slot and extends to one side along the end. According to one embodiment of the invention, the slot is perpendicular to the extending direction of the entering slot, a U-shaped groove structure is formed, and the slot and the entering slot form an L shape. According to one embodiment of the invention, one end of the fixed shade frame is provided with a mounting hole, and the shape and the size of the mounting hole are consistent with those of a probe of the Raman spectrometer, so as to accommodate and fix the probe. According to one embodiment of the invention, the mounting hole is internally provided with the internal measurement platform, the internal measurement platform is formed by protruding and extending from the periphery of the inner wall of the mounting hole to the central position, and is a certain distance from the upper port of the mounting hole, and the cross section size of a cavity below the internal measurement platform is smaller than the