CN-117581938-B - Preparation method for improving gel performance of sturgeon myofibrillar protein

CN117581938BCN 117581938 BCN117581938 BCN 117581938BCN-117581938-B

Abstract

The invention provides a preparation method for improving the gel performance of sturgeon myofibrillar proteins, and belongs to the technical field of food processing technology. The preparation method for improving the performance of the sturgeon myofibrillar protein gel comprises the following steps of extracting myofibrillar protein in sturgeon flesh to obtain a sturgeon myofibrillar protein solution, carrying out ultrasonic treatment and malondialdehyde induction treatment on the sturgeon myofibrillar protein solution to obtain composite myofibrillar protein, dialyzing the composite myofibrillar protein to obtain a modified protein solution, and carrying out heat induction on the modified protein solution to obtain the sturgeon myofibrillar protein gel. The preparation method can effectively improve the water retention and storage modulus of the sturgeon myofibrillar protein gel, so that the water retention capacity and deformation resistance of the gel are obviously enhanced, and the preparation method has an important effect on the application of the sturgeon myofibrillar protein in gel-based products.

Inventors

CHEN YUEWEN
YU WENTAO
SHEN SHIKE
JIN DANLI
YUAN YANWEI
FU JINGJING

Assignees

浙江工商大学

Dates

Publication Date: 20260508
Application Date: 20231207

Claims (4)

1. The preparation method for improving the gel performance of the sturgeon myofibrillar proteins is characterized by comprising the following steps of: (1) Extracting myofibrillar proteins in sturgeon flesh to obtain a sturgeon myofibrillar protein solution; (2) Firstly carrying out ultrasonic treatment on the sturgeon myofibrillar protein solution, and then carrying out malondialdehyde induction treatment to obtain composite myofibrillar protein; the ultrasonic treatment is carried out for 10-20 min at a temperature of 2-8 ℃ under a power of 500-700 w; During the induction treatment of the malondialdehyde, the use concentration of the malondialdehyde is 5-20 mmol/L, and the volume ratio of the malondialdehyde to the sturgeon myofibrillar protein solution is 1:5-20; The condition of the malondialdehyde induction treatment is that the malondialdehyde reacts for 20-28 hours at 22-25 ℃ in the dark; (3) Putting the composite myofibrillar protein into PBS dialysate for dialysis for 20-28 h to obtain modified protein solution; (4) Thermally inducing the modified protein solution to obtain sturgeon myofibrillar protein gel; The heat induction is to heat the modified protein solution from 22-25 ℃ to 72-77 ℃ at a speed of 1-1.5 ℃ per minute, and keep the temperature of the modified protein solution at 72-77 ℃ for 20-30 min.
2. The preparation method of the sturgeon myofibrillar crude protein is characterized by comprising the steps of mixing sturgeon flesh with a protein extract A according to a mass-volume ratio of 1:8-12, homogenizing, freezing and centrifuging, mixing the centrifuged sediment with the protein extract A according to a mass-volume ratio of 1:4-6, freezing and centrifuging again, taking the sediment to obtain the sturgeon myofibrillar crude protein, mixing the sturgeon myofibrillar crude protein with a protein extract B according to a mass-volume ratio of 1-2:1-2, carrying out ice bath after homogenizing, freezing and centrifuging again, and taking supernatant to obtain a myofibrillar protein solution; The protein extract A uses water as a solvent and comprises the following components with the final concentration of 10-14 mmol/L Na 2 HPO 4 12H 2 O,6~10 mmol/L NaH 2 PO 4 2H 2 O,90~110 mmol/L NaCl,0.5~1.5mmol/L EDTA; The protein extract B uses water as a solvent and comprises the following components in final concentration of 90-110 mmol/L NaCl and 12-17 mmol/L Na 2 HPO 4 12H 2 O,8~12mmol/L NaH 2 PO 4 2H 2 O。
3. The method according to claim 2, wherein the homogenization is carried out under 13000-15000 r/min for 60-90 s.
4. A sturgeon myofibrillar protein gel obtained by the method of any one of claims 1 to 3.

Description

Preparation method for improving gel performance of sturgeon myofibrillar protein Technical Field The invention relates to the technical field of food processing technology, in particular to a preparation method for improving the gel performance of sturgeon myofibrillar protein. Background Sturgeon protein is an animal protein rich in a plurality of essential amino acids and has good biological value. In recent years, the sturgeon breeding industry in China rapidly develops, but the processing and utilization of sturgeon is still at a low level, and how to reasonably utilize the sturgeon to realize the deep processing of the sturgeon myofibrillar proteins becomes a research hotspot for improving the application value of the sturgeon myofibrillar proteins. At present, the preparation of sturgeon myofibrillar proteins as gel-based products attracts the public's eye. However, the strength of the gel formed by the sturgeon protein is weak, which greatly limits the application of the sturgeon protein in gel-based products in the future. Malondialdehyde (MDA) is the most abundant active aldehyde in lipid oxidation secondary products in meat product processing and storage, and MDA has the functions of causing cross-linking polymerization of protein, decomposing and folding of protein structure and the like, and has wide application path and broad market prospect. Ultrasonic technology is an emerging technology with green and development potential. Sonication can alter the thiol content and secondary structure of the protein, resulting in changes in the emulsification and gelation properties of the myofibrillar proteins. In the face of the characteristics of sturgeon protein, how to improve the gel performance of sturgeon myofibrils has important significance for the preparation and production of food. Disclosure of Invention The invention aims to provide a preparation method for improving the gel performance of sturgeon myofibrillar proteins so as to improve the gel quality. In order to achieve the above object, the present invention provides the following technical solutions: the invention provides a preparation method for improving the gel performance of sturgeon myofibrillar proteins, which comprises the following steps: (1) Extracting myofibrillar proteins in sturgeon flesh to obtain a sturgeon myofibrillar protein solution; (2) Carrying out ultrasonic treatment and malondialdehyde induction treatment on the sturgeon myofibrillar protein solution to obtain composite myofibrillar protein; (3) Dialyzing the composite myofibrillar protein to obtain a modified protein solution; (4) And (3) carrying out heat induction on the modified protein solution to obtain the sturgeon myofibrillar protein gel. Preferably, the extraction method in the step (1) comprises the steps of mixing sturgeon meat with a protein extract A according to a mass-volume ratio of 1:8-12, homogenizing, freezing and centrifuging, mixing the centrifuged precipitate with the protein extract A according to a mass-volume ratio of 1:4-6, freezing and centrifuging again, taking the precipitate to obtain sturgeon myofibril crude protein, mixing the sturgeon myofibril crude protein with a protein extract B according to a mass-volume ratio of 1-2:1-2, homogenizing, carrying out ice bath, freezing and centrifuging again, and taking the supernatant to obtain a myofibril protein solution. Preferably, the protein extract A is prepared by using water as solvent, and comprises the following components in final concentration ：10～14mmol/L Na2HPO4˙12H2O,6～10mmol/L NaH2PO4˙2H2O,90～110mmol/LNacl,0.5～1.5mmol/L EDTA. Preferably, the homogenization is carried out under the condition of 13000-15000 r/min for 60-90 s. Preferably, the protein extract B is prepared by using water as solvent, and comprises the following components in final concentration ：90～110mmol/L Nacl,12～17mmol/L Na2HPO4˙12H2O,8～12mmol/LNaH2PO4˙2H2O. Preferably, the ultrasonic treatment in the step (2) is carried out for 10-20 min at a power of 500-700 w and a temperature of 2-8 ℃. Preferably, during the induction treatment of the malondialdehyde in the step (2), the use concentration of the malondialdehyde is 5-20 mmol/L, the volume ratio of the malondialdehyde to the sturgeon myofibrillar protein solution is 1:5-20, and the treatment condition is that the reaction is carried out for 20-28 h at 22-25 ℃ in a dark place. Preferably, in the step (3), the dialysis is performed by placing the complex myofibrillar proteins in PBS dialysate for 20-28 hours. Preferably, in the step (4), the modified protein solution is heated from 22-25 ℃ to 72-77 ℃ at a speed of 1-1.5 ℃ per minute, and kept at the temperature of 72-77 ℃ for 20-30 min. The invention also provides sturgeon myofibrillar protein gel obtained by the preparation method. The preparation method for improving the gel performance of the sturgeon myofibrillar protein has the following beneficial effects: (1) Improving sturgeon myofibrillar protein gel p