Search

CN-117643301-B - Combined regulator for relieving long-term low-temperature stress and promoting germination of rice seeds and application

CN117643301BCN 117643301 BCN117643301 BCN 117643301BCN-117643301-B

Abstract

The invention relates to the technical field of agricultural planting, and particularly discloses a combined regulator for relieving long-term low-temperature stress and promoting rice seed germination and application thereof, wherein the effective components of the combined regulator consist of 5-10mg/L gibberellin and 0.4-0.6% Chitosan (CS). The optimal initiator is selected by adopting a method of combining the non-hormonal regulator and the osmotic regulator, namely 10mg/L gibberellin and 0.5% chitosan combined regulator, so that the mixed initiator not only has higher germination rate (94.67%) in low-temperature environment, but also has higher germination index (40.63), and the germination index is obviously larger than that of the common regulator, and the mixed initiator is particularly suitable for being used in areas with longer duration of 'cold in the back spring' or longer duration of low temperature in sowing seasons.

Inventors

  • ZHANG XIAOLI
  • HUANG BINGCHUAN
  • ZHANG HUA
  • Liang tianfeng
  • GAO GUOQING
  • GUO HUI
  • TANG MAOYAN
  • TAO WEI
  • CHEN LEI
  • ZHONG XIAOYUAN

Assignees

  • 广西壮族自治区农业科学院

Dates

Publication Date
20260512
Application Date
20231206

Claims (8)

  1. 1. A combined regulator for relieving long-term low-temperature stress and promoting germination of rice seeds is characterized in that the effective components of the combined regulator consist of gibberellin and chitosan, the seed soaking concentration of the gibberellin is 10mg/L, and the seed soaking concentration of the chitosan is 0.5%.
  2. 2. A method of promoting germination of rice seeds using the combination modulator of claim 1, comprising the steps of: (1) Initiating treatment, namely selecting full rice seeds with high temperature resistance, soaking the rice seeds in the combined regulator, and initiating the rice seeds for 24 hours at the temperature of 12-15 ℃; (2) Drying, namely filling rice seeds into a mesh bag after initiation is finished, flushing with tap water, draining, and drying at a constant temperature of 25 ℃; (3) Sowing, namely directly sowing rice seeds after initiating drying or placing the rice seeds into a self-sealing bag and preserving the rice seeds in a refrigerator at a temperature of 4 ℃ for standby.
  3. 3. The method of claim 2, further comprising the step of (4) germinating by counting germination conditions of each combination, including germination rate, germination index and germination length, and scanning the germinated plants.
  4. 4. The method of claim 2, wherein in step (1), the low temperature resistant rice seeds are yellow rice seeds, the low temperature resistant rice seeds are sterilized with 1% of strong chlorine for 20min, and then washed clean with water and then soaked in a combination regulator.
  5. 5. The method of claim 2, wherein in step (1), the ratio of the mass of the low temperature resistant rice seed to the volume of the combined regulator is 1:4-6 (w/v).
  6. 6. The method according to claim 2, wherein in step (1), the rice seeds resistant to temperature are induced under dark conditions of 12 ℃ for 12 hours and under light conditions of 15 ℃ for 12 hours.
  7. 7. The method according to claim 2, wherein in the step (2), the tap water is washed for 3min, and the drying operation is carried out by placing the mixture in a constant temperature drying oven at 25 ℃ for drying for 48h.
  8. 8. The method of claim 2, wherein in step (3), the rice seeds after the initiation of drying are sown into the paddy field by mechanical direct seeding.

Description

Combined regulator for relieving long-term low-temperature stress and promoting germination of rice seeds and application Technical Field The invention belongs to the technical field of agricultural planting, and particularly relates to a combined regulator for relieving long-term low-temperature stress and promoting germination of rice seeds and application thereof. Background Rice (Oryza sativa L.) is the most important grain crop in China, but due to low economic benefit, the sowing area of rice is continuously reduced, and the phenomenon that double-cropping rice is changed into single-cropping rice and even early-season rice is abandoned is very common in Guangxi province. The biggest cost of rice planting is seedling transplanting, and the production cost of seedling transplanting links accounts for more than 30% of the total cost of rice production no matter manual seedling throwing, transplanting or mechanical transplanting. The mechanical direct seeding of rice omits the links of seedling raising, seedling conveying and transplanting, greatly reduces the cost and saves the labor force. In recent years, with the continuous expansion of rural land circulation scale, the national support for standardized farmland construction is added, land drought changes water and small changes are greatly improved, favorable conditions are provided for mechanized precise direct seeding planting of rice, and mechanized direct seeding of rice is also widely concerned and adopted. At present, the main problem encountered in direct seeding rice popularization is that the low temperature caused by early-stage 'cold in the back spring' and low illumination of overcast and rainy days seriously affects the emergence quality of the direct seeding rice, is difficult to 'one-seeding full-seedling', and directly affects the yield of the direct seeding rice. The problem can be solved by breeding low temperature resistant varieties suitable for direct seeding, but the breeding period is longer, and the low temperature resistant varieties are difficult to use rapidly. The simplest and effective solution at present is to increase the emergence rate by combining the regulator. The team applies for a patent (202210237085. X) for promoting germination of direct seeding early rice seeds under low temperature stress before, and adopts GA 3 +SA to jointly trigger treated seeds, so that germination rate and emergence rate of the rice seeds under low temperature stress can be improved, one-seeding full-seedling of direct seeding early rice is ensured, and rapid development of direct seeding rice is promoted. However, the germination index of the seeds treated by the GA 3 and SA together is not high, and the germination rate and the emergence rate can be affected in areas with severe environment and longer duration of the cold in the back spring. The team also optimizes the formula of the combined regulator, combines the hormone regulator and the penetration regulator, can obtain the regulator with higher germination rate and germination index, has obvious advantages in the areas with more severe environment and longer duration of the reverse spring cold, provides technical support for the direct seeding popularization of early rice, and has important significance for promoting the mechanized direct seeding of the rice. Disclosure of Invention The invention aims to provide a combined regulator for relieving long-term low-temperature stress and promoting germination of rice seeds and application thereof, thereby overcoming the defects that the existing rice direct seeding is used in a region with a severe environment and a long duration of 'cold in the back spring', the rice seeds are easily affected by low-temperature stress, the germination rate is low and the seeds are easily rotten. In order to achieve the aim, the invention provides a combined regulator for relieving long-term low-temperature stress and promoting rice seed germination, wherein the effective components of the combined regulator consist of gibberellin and chitosan, the seed soaking concentration of the gibberellin is 5-10mg/L, and the seed soaking concentration of the chitosan is 0.4-0.6%. The gibberellin is a hormone type initiator, and the chitosan is a permeation initiator, so that the combination of the gibberellin and the chitosan can activate plant resistance, improve various germination indexes of seeds, and reduce adverse effects of abiotic stress on seed germination. The practice proves that the chitosan and gibberellin can resist low temperature for a long time, and is particularly suitable for being used in areas with longer duration of 'cold in the back spring'. Preferably, the seed soaking concentration of the gibberellin is 10mg/L, and the seed soaking concentration of the chitosan is 0.5%. Another technical scheme of the application is as follows: A method for promoting germination of rice seeds by adopting the combined regulator comprises the following st