CN-117723728-B - Rapid restarting biotoxicity online monitoring equipment and method

CN117723728BCN 117723728 BCN117723728 BCN 117723728BCN-117723728-B

Abstract

The invention provides quick restarting biotoxicity on-line monitoring equipment and a quick restarting biotoxicity on-line monitoring method, and belongs to the technical field of water body monitoring. The microbial feedback system comprises an anode chamber, an intermediate chamber, a cathode chamber and a sewage input pump, wherein the intermediate chamber is arranged between the anode chamber and the cathode chamber, a biological anode is arranged between the anode chamber and the intermediate chamber, a biological cathode is arranged between the intermediate chamber and the cathode chamber, the sewage input pump is connected with an anode chamber water inlet port, a bacterial liquid water inlet port is arranged on one side close to the anode chamber, a backwashing port is arranged on one side close to the cathode chamber, a bacterial liquid water inlet pump is connected with a bacterial liquid water inlet port, and a clear water backwashing pump is connected with the backwashing port. The invention can rapidly form an electroactive biomembrane, and different current responses are generated by utilizing a microbial electrochemical system, so that the real-time on-line monitoring of toxic substances in water quality is realized.

Inventors

FENG HUAJUN
LIANG YUXIANG
LI PINGLI
YE FANGFANG
ZHANG YANQING
XIANG HAI
DING YANGCHENG
Qiu Xiawen

Assignees

浙江农林大学

Dates

Publication Date: 20260512
Application Date: 20240125

Claims (10)

1. The quick restarting biotoxicity online monitoring device is characterized by comprising a signal acquisition system, a microbial feedback system and a quick restarting system; The signal acquisition system comprises an electrochemical workstation or a regulated power supply, wherein the electrochemical workstation or the regulated power supply is electrically connected with a biological anode in the microbial feedback system and is used for applying electric potential to the biological anode; the microbial reactor comprises an anode chamber, an intermediate chamber and a cathode chamber, wherein the intermediate chamber is arranged between the anode chamber and the cathode chamber; a biological anode is arranged between the anode chamber and the middle chamber, and a biological cathode is arranged between the middle chamber and the cathode chamber; The quick restarting system comprises a bacterial liquid water inlet pump and a clear water backwashing pump, wherein the bacterial liquid water inlet pump is connected with the bacterial liquid water inlet port of the microbial reactor, and the clear water backwashing pump is connected with the backwashing port.
2. The rapid restart on-line biotoxicity monitoring device of claim 1, wherein the microbial reactor volume V is 4-50 ml, and wherein the anode chamber volume V1: intermediate chamber volume V2: cathode chamber volume V3 is 3-10:1-2:1-2.
3. The rapid restart biotoxicity online monitoring device of claim 2 wherein the bioanode and biocathode materials are one or more of titanium, stainless steel, carbon fiber and conductive polymers.
4. The rapid restarting biological toxicity on-line monitoring apparatus of claim 2 wherein the bioanode and the biocathode are porous network structures, the bioanode pore diameter is 1000-12500 mesh, and the biocathode pore diameter is 10-100 mesh.
5. A rapid restarting biotoxicity online monitoring method, characterized in that it is implemented by the rapid restarting biotoxicity online monitoring device according to any one of claims 2 to 4, comprising the following steps: the pre-starting stage comprises the steps of culturing conductive bacterial liquid through a bioelectrochemical reactor; s2, a quick starting stage, namely inputting cultured bacterial liquid into a microbial reactor through a bacterial liquid water inlet pump, running for 20-50 minutes, forming a biological film on the surface of a biological anode through filtration, and simultaneously applying positive potential to strengthen the adsorption of microorganisms in the bacterial liquid to the surface of the biological anode; S3, in the water quality monitoring stage, sewage to be monitored is input into the microbial reactor from the water inlet through the sewage input pump, and electric potential is applied to the biological anode to continuously monitor the sewage quality; s4, in the poisoning death stage, when the wastewater enters the microbial reactor and the current drops, the manager judges that toxic wastewater enters according to the data; And S5, in the restarting stage of the equipment, a water inlet port of the anode chamber is closed, clear water is input to a backwashing port of the microbial reactor through a clear water backwashing pump, dead bacterial liquid is discharged from the bacterial liquid water inlet port, and then the S1-S4 stages are repeated.
6. The method according to claim 5, wherein the inflow rate q1=v1/t×a during the rapid start phase, wherein T is a residence time in the range of 10-30, and a is a bacterial liquid concentration OD600 in the range of 0.05-0.3.
7. The rapid restart biotoxicity online monitoring method of claim 5 wherein the inflow rate q2=v1/T during the water quality monitoring phase, wherein T ranges from 2 to 5.
8. The rapid restart biotoxicity online monitoring method of claim 5, wherein backwash flow rate q3= (v2+v3)/T during the plant restart phase, wherein T ranges from 0.5 to 1.5.
9. The rapid restarting biological toxicity on-line monitoring method according to claim 5, wherein the biological anode potential range in the rapid starting stage is 0-0.5V, the biological anode potential range in the water quality monitoring stage is-0.2-0V, and the biological anode potential range in the equipment restarting stage is-2.0 to-1.0V.
10. The method for on-line monitoring of biotoxicity of a rapid restart according to claim 5, wherein the water inlet time of the rapid start-up phase is 20-50 minutes, the backwash time of the equipment restart phase is 3-5 minutes, and the restart time after the poisoning death of the whole reactor is 23-55 minutes.

Description

Rapid restarting biotoxicity online monitoring equipment and method Technical Field The invention relates to the technical field of water body monitoring, in particular to quick restarting biotoxicity on-line monitoring equipment and a quick restarting biotoxicity on-line monitoring method. Background Water is one of the most important material resources essential for human life and development, and is also the most important material required for human life. However, with the development of industrialization and town, the total amount of industrial wastewater, agricultural water withdrawal and domestic sewage is gradually increased, and the use value of water is reduced or lost due to the existence of toxic and harmful substances, so that the water environment is polluted. For example, acids, alkalis, oxidizing agents, copper, cadmium, mercury, arsenic and other compounds in sewage, benzene, dichloroethane, ethylene glycol and other organic poisons can poison aquatic organisms and pollute drinking water sources. In addition, the water pollution not only can influence the growth and development of plants, but also can influence the reproductive system, the respiratory system and the nervous system of human bodies, thereby bringing hidden danger to the health and safety of human beings. Therefore, monitoring of water toxicity is critical to understanding water pollution. In recent years, the poison monitoring method based on the microorganism sensing technology can realize real-time early warning of water pollution according to the change of metabolism or growth speed when microorganisms are stimulated by the poison. However, due to the lack of a transducer, such biosensors do not convert biological signals into electrical signals, and thus lack the ability to quantitatively monitor specific concentrations of poisons. The principle of detecting the biotoxicity of the water quality of the water body by the microbial electrochemical system is that toxic pollutants in the water body inhibit the respiratory metabolism of electrochemically active microorganisms, so that the extracellular electronic transmission process is influenced, the electric signal of the microbial electrochemical system is changed, and the online monitoring of the biotoxicity of the water quality of the water body is realized according to the change of the electric signal of the microbial electrochemical system. However, in the microbial electrochemical system, the electrochemically active microorganisms generally form an electroactive biofilm and adhere to the electrode, but a lot of time is required, if a substance with high toxicity exists in the water body, the activity of the microorganisms forming the electroactive biofilm is reduced or dead, a new electroactive biofilm cannot be cultivated in time, and the time for re-cultivating the electroactive biofilm is long. The patent CN202211092024.5 is used for water quality monitoring by combining a water quality monitoring system and a microbial sensor, wherein the microbial sensor uses electrochemical active microorganisms as tested organisms to detect and identify corresponding substrates by utilizing various enzyme systems and metabolic systems in the microorganisms, and can test the comprehensive toxicity of toxic substances. The technology does not consider the situation that an electroactive biological film is damaged by toxic substances in a water body and microorganisms are killed, and does not consider the problem of restarting a system after the death of the tested organisms. Patent CN202111505254.5 couples a traditional microbial electrochemical system with a novel dynamic membrane separation process, and promotes the adsorption and deposition of electroactive organisms on the surface of a macroporous conductive substrate through interception, mass transfer enhancement and electrostatic enhancement, thereby accelerating the film forming process. The mesh number of the anode of the macroporous conductive substrate selected by the technology is too large, and an electroactive biological film is not easy to form on the anode when microorganisms are filtered, so that the film forming speed is improved, but still needs to be enhanced. Based on the analysis, a rapid forming method is designed for cultivating the electroactive biological film, the real-time monitoring of the toxicity of the water body is realized by means of an electric signal, the purpose of rapidly and long-effectively knowing the toxic pollution of the water body and timely providing a corresponding treatment strategy for the water body pollution is achieved, and a technical means is provided for the treatment and toxicity monitoring of the water pollution. Disclosure of Invention Aiming at the problems that the cultivation of the electroactive biological film is slow, whether the biological film is damaged or not is difficult to know, the time consumption for cultivating the electroactive biological film again is l