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CN-117730804-B - Method for cultivating delicious euglena seedlings

CN117730804BCN 117730804 BCN117730804 BCN 117730804BCN-117730804-B

Abstract

The invention discloses a method for cultivating a delicious euglena larva, and relates to the technical field of mariculture. The method comprises the steps of (1) parent cultivation, (2) induced spawning, (3) sea ball larva cultivation, (4) preparation of a benthonic cultivation pool, (5) planktonic larva induction metamorphosis, and (6) benthonic larva cultivation management. The invention can effectively improve the efficiency of feeding sea mud for the creeping larvae, improve the dissolved oxygen in the sediment substrate, and reduce the conditions of low dissolved oxygen and high death rate caused by ponding in the drying process.

Inventors

  • PENG HUIJING
  • ZOU JIE
  • SONG JIANQIANG
  • DENG ZHAOJUN

Assignees

  • 广西海洋研究所有限责任公司
  • 广西兰石海洋科技有限公司

Dates

Publication Date
20260505
Application Date
20240205

Claims (7)

  1. 1. The method for cultivating the delicious euglena seedlings is characterized by comprising the following steps of: (1) Parent cultivation, namely purchasing a wild delicious euglena adult in 3-5 months, digging and cleaning after the parent cultivation pond is cultivated to 8 months, and selecting an individual with more than 2/3 of the body surface of the euglena adult to be a parent for hastening parturition; The body surface of the wild delicious euglena adult is not damaged, the body is yellow brown, the parent is kept stand for 1min, the kiss-roll stretches out, and the individual is quickly retracted after being disturbed; The culture process comprises the steps of putting the adult into a parent culture pond after washing, feeding and draining water once every 5 days, and feeding and draining water once every 5 days after washing, not feeding the adult in the parent culture pond for the first 5 days, spraying benthic diatom liquid and sea cucumber feed after draining after the adult is fed and drained, wherein the feeding ratio is 1:2 of dry mass, feeding water after 1-3 hours of dry dew, draining water 5:00 a day, and feeding water 8:00 a day; (2) The spawning is induced, namely 8-10 months, the delicious euglena enters a breeding full period, parents are selected to induce spawning, a large number of spawns are produced and fertilized, and after 30-45 hours of hatching, the hatching temperature is 25-33 ℃; (3) Sea ball larva cultivation, namely, sea ball larva cultivation density is 0.2-0.4/ml, cultivation is carried out in a cultivation pond for 14d, the length and ingestion condition of the sea ball larva are observed, daily water changing amount is 30%, and when the length of an individual body reaches 380 mu m, the sea ball larva is transferred into a benthonic cultivation pond for cultivation; (4) Preparing a benthonic cultivation pond, namely customizing an 80-mesh bolting silk net with the specification of 4.5m multiplied by 5.0m, paving a sponge with the thickness of 0.3cm on the bottom of the cultivation pond, paving the whole pond, covering the upper layer of the sponge with 0.2cm of fine sand, the grain diameter of the fine sand is less than or equal to 0.5mm, covering the surface layer with sea mud with 0.05cm, the grain diameter of the fine sand is less than or equal to 0.05mm, the sedimentation rate is less than or equal to 0.95mm/s, soaking the bottom material for 1d by adding water, draining water and drying in shade for 4h after the bottom material is paved, repeating the operation for 3 times, culturing Chi Guangzhao by a shading net, lighting for 1000-1500 lux, adding water and micro-inflating before the larvae are transferred to the benthonic cultivation pond, and sprinkling probiotics agent and syrup with the mass ratio of 100ml of mixed solution for directional cultivation benthonic diatom with the DO value of more than or equal to 3mg/L each day, and when the bottom material surface is yellowish, pouring sea ball larvae into the bottom material pond, installing a spray head for 2 sea water spray on the pond, and cooling and wetting the bottom material by using rotary spray; (5) After the sea ball larvae are transferred to a benthic cultivation pond, the density of the seedlings is 0.04 per mL, the water level of the benthic cultivation pond is 50cm, the daily temperature difference is 5 ℃, trace aeration is carried out, the sea ball larvae are fed by the sea chain algae of Wei, the daily feeding amount is 6-10 multiplied by 10 8 cells, and the daily water changing amount is 30%; (6) The cultivation management of benthonic larvae comprises the steps that after the larvae are completely creeping and metamorphosed, more than 60% of the larvae in intestinal tracts can be observed under a microscope, the mixture of mud and diatoms is drained from a cultivation pond after 18:00 a day, the temperature in the cultivation pond is 25-33 ℃ when the larvae are dried in the shade, 2 spray heads above the cultivation pond spray seawater to cool and moisten the bottoms when the temperature exceeds 33 ℃, benthonic diatom liquid and sea cucumber offspring seed feed are splashed during the shade drying, the feeding amount is reasonably splashed according to larva feeding conditions, after the larvae grow to 0.3cm, sediment on each sponge is turned into a red basin, the sponge is taken out, the larvae in the benthonic cultivation pond are washed and collected, and then transferred to an intermediate cultivation pond, and cultivation is carried out after the whole pond is splashed.
  2. 2. The cultivation method according to claim 1, wherein in the parent cultivation pond in the step (1), sediment with 20cm is spread at the bottom, the mud content is more than 50%, ditching and ridging are carried out, the width of each ridge is 100 cm-150 cm, and the width of each ditch is 15 cm-20 cm.
  3. 3. The cultivation method according to claim 1, wherein the induced spawning in the step (2) is to place the parent on a cement floor for drying in the shade for 4 hours at a humidity of 30% -50% and a temperature of 25 ℃, and then place the parent in a parent cultivation pool after drying in the shade.
  4. 4. The cultivation method according to claim 1, wherein after the larvae are hatched, golden algae and chaetoceros are firstly opened by golden algae, then fed, the feeding amount of golden algae on day 1 is 80% of the total cell amount fed, the feeding amount is gradually decreased by 10% on day 6, the feeding amount of flat algae is gradually increased, the total cell amount fed is 10% -30%, the feeding ratio of golden algae is not lower than the total cell amount fed by 10%, and the feeding amount of golden algae is 8-40×10 8 cells.
  5. 5. The cultivation method according to claim 1, wherein in the step (4), the fine sand has a particle size of more than 0.055mm and less than 0.150 sand particles exceeding 85% by weight and an average particle size of 0.075mm to 0.100mm.
  6. 6. The method according to claim 1, wherein in the step (4), the sponge is 45 cm. Times.45 cm. Times.0.3 cm and the weight is 1000g.
  7. 7. A cultivation method according to claim 1, wherein in step (4), the sea mud is obtained from muddy beach, after exposure to sun, the mud is soaked in sea water, washed with 80 mesh screen cloth, and deposited, and the upper sea water is poured off to obtain surface mud.

Description

Method for cultivating delicious euglena seedlings Technical Field The invention relates to the technical field of mariculture, in particular to a method for cultivating a delicious euglena larva. Background The delicious sipunculus nudus (Phascolosomaesculenta) belongs to the sipunculus nudus animal phylum (Sipuncula) and the sipunculus nudus family (Phascolosomida), and is a special species in China and an emerging mariculture species. The animals inhabit the intertidal zone mud, are distributed in the camping and hole living, are distributed in the southeast coast, and along with the rapid development of the breeding industry, natural offspring seeds cannot meet the breeding requirements of farmers, and the production scale is limited due to the fact that the animals depend on wild offspring seeds excessively. With the basic research of the delicious euglena, the key technology of cultivating and inducing metamorphosis of sea ball larvae breaks through one by one, but the cultivation management in the late metamorphosis period and the cultivation management in the middle cultivation are still technical bottlenecks for realizing the large-scale breeding of offspring seeds due to uncontrollable physical and chemical factors of sediment substrates. Because the delicious euglena grows in the sediment substrate, clean sediment cannot be obtained through cleaning, the cleanliness of the obtained sediment is difficult to control, the sediment contains high content of dormant eggs, small-specification organisms, bacteria and ammonia nitrogen of other marine organisms, and the artificial seedling raising effect of taking the sediment from a natural sea area is poor. Therefore, providing a method for cultivating the seedlings of the delicious euglena is a problem which needs to be solved by the person skilled in the art. Disclosure of Invention In view of the above, the invention provides a method for cultivating the seedlings of the Phascota to be tasty. In order to achieve the above purpose, the present invention adopts the following technical scheme: A method for cultivating a delicious euglena seedling comprises the following steps: (1) Parent cultivation, namely purchasing a wild delicious euglena adult in 3-5 months, digging and cleaning after the parent cultivation pond is cultivated to 8 months, and selecting an individual with more than 2/3 of the body surface of the euglena adult to be a parent for hastening parturition; (2) The spawning is induced, namely 8-10 months, the delicious euglena enters a breeding full period, parents are selected to induce spawning, a large number of spawns are produced and fertilized, and after 30-45 hours of hatching, the hatching temperature is 25-33 ℃; (3) Sea ball larva cultivation, namely, sea ball larva cultivation density is 0.2-0.4/ml, cultivation is carried out in a cultivation pond for 14d, the length and ingestion condition of the sea ball larva are observed, daily water changing amount is 30%, and when the length of an individual body reaches 380 mu m, the sea ball larva is transferred into a benthonic cultivation pond for cultivation; (4) Preparing a benthonic cultivation pond, namely customizing an 80-mesh bolting silk net with the specification of 4.5m multiplied by 5.0m, paving a sponge with the thickness of 0.3cm on the bottom of the cultivation pond, paving the whole pond, covering the upper layer of the sponge with 0.2cm of fine sand, the grain diameter of the fine sand is less than or equal to 0.5mm, covering the surface layer with sea mud with 0.05cm, the grain diameter of the fine sand is less than or equal to 0.05mm, the sedimentation rate is less than or equal to 0.95mm/s, soaking the bottom material for 1d by adding water, draining water and drying in shade for 4h after the bottom material is paved, repeating the operation for 3 times, culturing Chi Guangzhao by a shading net, lighting for 1000-1500 lux, adding water and micro-inflating before the larvae are transferred to the benthonic cultivation pond, and sprinkling probiotics agent and syrup with the mass ratio of 100ml of mixed solution for directional cultivation benthonic diatom with the DO value of more than or equal to 3mg/L each day, and when the bottom material surface is yellowish, pouring sea ball larvae into the bottom material pond, installing a spray head for 2 sea water spray on the pond, and cooling and wetting the bottom material by using rotary spray; (5) After the sea ball larvae are transferred to a benthic cultivation pond, the density of the seedlings is 0.04 per mL, the water level of the benthic cultivation pond is 50cm, the daily temperature difference is 5 ℃, trace aeration is carried out, the sea ball larvae are fed by the sea chain algae of Wei, the daily feeding amount is 6-10 multiplied by 10 8 cells, and the daily water changing amount is 30%; (6) The cultivation management of benthonic larvae comprises the steps that after the larvae are completely creeping and metam