CN-117752833-B - Alkali liquor sterilization method and device for fermentation sampling liquid

CN117752833BCN 117752833 BCN117752833 BCN 117752833BCN-117752833-B

Abstract

The invention provides an alkali liquor sterilization method and device for fermentation sampling liquor, which are applied to sterilization equipment, wherein the sterilization equipment comprises a sterilization tank, a pH value measuring device, a stirring device and an alkali liquor input device, the stirring device and the pH value measuring device are assembled in the sterilization tank, the method comprises the steps of controlling the alkali liquor input device to input alkali liquor into the sterilization tank in batches, controlling the stirring device to stir the liquid in the sterilization tank, measuring the pH value of the liquid in the sterilization tank through the pH value measuring device, and controlling the alkali liquor input device to stop inputting the alkali liquor when the time length of the pH value measured by the pH value measuring device exceeding a first preset threshold reaches a third preset time length, controlling the stirring device to stop stirring, and discharging the liquid in the sterilization tank.

Inventors

DU YING
ZHANG CHUNLEI
WU SHICHENG
XIE MIN
DENG DINGMING

Assignees

中控技术股份有限公司

Dates

Publication Date: 20260505
Application Date: 20231229

Claims (10)

1. The alkali liquor sterilization method for the fermentation sampling liquid is characterized by being applied to sterilization equipment, wherein the sterilization equipment comprises a sterilization tank, a pH value measuring device, a stirring device and an alkali liquor input device, the stirring device and the pH value measuring device are assembled in the sterilization tank, and the method comprises the following steps: Controlling the alkali liquor input device to input alkali liquor into the sterilization tank in batches in response to the sampling liquor in the sterilization tank reaching a preset liquid level, wherein the time length of each alkali liquor input by the alkali liquor input device is a first preset time length; controlling the stirring device to stir the liquid in the sterilization tank, and measuring the pH value of the liquid in the sterilization tank through the pH value measuring device, wherein in the process of inputting alkali liquor in batches by the alkali liquor input device, the condition of inputting the alkali liquor again is that the pH value measured by the pH value measuring device is unchanged within a second preset time period; And under the condition that the time length of the PH value measured by the PH value measuring device exceeding the first preset threshold value reaches the third preset time length, controlling the alkali liquid input device to stop inputting alkali liquid, controlling the stirring device to stop stirring, and discharging the liquid in the sterilization tank.
2. The method of claim 1, wherein the sterilization apparatus further comprises a fluid level measurement device and a sample fluid input device, the method further comprising: Inputting a sampling liquid into the sterilization tank through the sampling liquid input device; And measuring the liquid level of the sampling liquid input into the sterilization tank through the liquid level measuring device, and controlling the sampling liquid input device to stop inputting the sampling liquid under the condition that the measured liquid level of the sampling liquid reaches the preset liquid level.
3. The method of claim 2, wherein the sample fluid input device comprises a flow control switch and a flow measurement device, the method further comprising: measuring the flow of the sampling liquid input by the sampling liquid input device through the flow measuring device; Controlling the flow control switch to reduce the flow of the sampling liquid input by the sampling liquid input device under the condition that the measured flow exceeds a first preset flow value; and under the condition that the measured flow is smaller than a second preset flow value, controlling the flow control switch to increase the flow of the sampling liquid input by the sampling liquid input device.
4. The method according to claim 1, wherein the method further comprises: And under the condition that the pH value of the liquid reaches a second preset threshold value, modifying the time length of each alkali liquid input by the alkali liquid input device into a fourth preset time length, wherein the first preset threshold value is larger than the second preset threshold value, and the first preset time length is longer than the fourth preset time length.
5. The method of claim 1, wherein the sterilization apparatus further comprises a pump connected to a drain port of the sterilization tank via a drain pipe, the drain pipe being provided with a pneumatic switch ball valve, and wherein draining the liquid from the sterilization tank comprises: And opening the pneumatic switch ball valve, and starting the material pump so that the material pump pumps out the liquid in the sterilization tank.
6. The method of claim 1, wherein the stirring device is a magnetic stirrer.
7. A sterilization apparatus, the sterilization apparatus comprising: A sterilizing tank for carrying a sample liquid to be sterilized and an alkali liquid for sterilization; the alkali liquor input device is connected with the sterilization tank and is used for inputting alkali liquor to the sterilization tank in batches in response to the fact that the sampling liquor in the sterilization tank reaches a preset liquid level, and stopping inputting the alkali liquor when the time length of the pH value exceeding a first preset threshold value measured by the pH value measuring device reaches a third preset time length; The pH value measuring device is assembled in the sterilization tank and used for measuring the pH value of liquid in the sterilization tank, wherein in the process of inputting alkali liquor in batches by the alkali liquor input device, the condition of inputting the alkali liquor again is that the pH value measured by the pH value measuring device is unchanged within a second preset time period; and the stirring device is assembled in the sterilization tank and is used for stirring the liquid in the sterilization tank, and the stirring device stops stirring when the time length of the PH value measured by the PH value measuring device exceeding the first preset threshold value reaches the third preset time length.
8. The alkali liquor sterilization device for the fermentation sampling liquid is characterized by being applied to sterilization equipment, wherein the sterilization equipment comprises a sterilization tank, a pH value measuring device, a stirring device and an alkali liquor input device, the stirring device and the pH value measuring device are assembled in the sterilization tank, and the device comprises: The first control unit is used for controlling the alkali liquor input device to input alkali liquor into the sterilization tank in batches in response to the fact that the sampling liquor in the sterilization tank reaches a preset liquid level, wherein the time length of each time of alkali liquor input by the alkali liquor input device is a first preset time length; The second control unit is used for controlling the stirring device to stir the liquid in the sterilization tank and measuring the pH value of the liquid in the sterilization tank through the pH value measuring device, wherein in the process of inputting the alkali liquor in batches through the alkali liquor input device, the condition of inputting the alkali liquor again is that the pH value measured by the pH value measuring device is unchanged within a second preset time period; And the liquid draining unit is used for controlling the alkali liquid input device to stop inputting the alkali liquid, controlling the stirring device to stop stirring and draining the liquid in the sterilization tank under the condition that the time length of the pH value exceeding the first preset threshold value measured by the pH value measuring device reaches the third preset time length.
9. An electronic device, comprising: A processor; A memory for storing processor-executable instructions; Wherein the processor is configured to implement the method of any one of claims 1 to 6 by executing the executable instructions.
10. A computer readable storage medium, on which a computer program is stored, characterized in that the program, when being executed by a processor, implements the steps of the method according to any one of claims 1 to 6.

Description

Alkali liquor sterilization method and device for fermentation sampling liquid Technical Field The invention relates to the field of sterilization, in particular to an alkali liquor sterilization method and device for fermentation sampling liquor. Background The microbial liquid fermentation process is a complex process involving microorganisms. The biochemical index including the concentration of glucose as a fermentation raw material and the concentrations of various fermentation products and fermentation intermediates is an extremely important key parameter in the fermentation process. The method is characterized in that regular sampling is needed after fermentation begins in large-scale fermentation industrial production or pilot plant production and laboratory production, the growth condition of microorganisms, namely the concentration (OD value) of thalli, the consumption condition of each component in a culture medium, particularly a carbon-nitrogen source, and the generation condition of metabolites in the growth process are conveniently mastered, and each biochemical index in the culture process is judged by knowing each parameter in the fermentation process, so that whether the growth condition of microorganisms is polluted, whether material supplementing is needed or not is judged, and the like, and the sampling operation is a very important link in the fermentation process. In order to avoid adverse effect of thalli on the environment, the fermentation workshop strictly prohibits living body emission, namely, the sampling liquid needs to be subjected to sterilization treatment and then is discharged. In the related art, the treatment is generally performed by a wet heat sterilization mode, a sampling sterilization tank with a larger volume is arranged in the mode, sampling liquid is input into the sterilization tank, saturated steam is introduced, timing is started after a certain temperature is reached, and the sampling liquid in the default sterilization tank is sterilized after a certain period of time and can be discharged. However, this approach requires the operator to participate in the sterilization operation, and during steam sterilization the operator needs to pay attention to the temperature change of the sterilization tank all the time, preventing the steam valve from being not closed for a long time and resulting in over-temperature overpressure, which in turn causes damage to the sterilization equipment. Therefore, the mode is dangerous, and risks of overtemperature and overpressure, equipment damage, scalding and the like caused by untimely operation during temperature rise can be generated. In addition, in order to reduce heat dissipation and improve the steam utilization rate, the sterilization tank needs to be kept warm, which increases the investment cost of equipment. Disclosure of Invention In view of the above, the present invention provides a method and apparatus for sterilizing alkaline solution of fermentation sample solution to solve the shortcomings in the related art. Specifically, the invention is realized by the following technical scheme: According to a first aspect of the invention, there is provided an alkali liquor sterilization method for fermentation sampling liquor, which is applied to sterilization equipment, wherein the sterilization equipment comprises a sterilization tank, a pH value measuring device, a stirring device and an alkali liquor input device, the stirring device and the pH value measuring device are assembled in the sterilization tank, and the method comprises the following steps: Controlling the alkali liquor input device to input alkali liquor into the sterilization tank in batches in response to the sampling liquor in the sterilization tank reaching a preset liquid level, wherein the time length of each alkali liquor input by the alkali liquor input device is a first preset time length; controlling the stirring device to stir the liquid in the sterilization tank, and measuring the pH value of the liquid in the sterilization tank through the pH value measuring device, wherein in the process of inputting alkali liquor in batches by the alkali liquor input device, the condition of inputting the alkali liquor again is that the pH value measured by the pH value measuring device is unchanged within a second preset time period; And under the condition that the time length of the PH value measured by the PH value measuring device exceeding the first preset threshold value reaches the third preset time length, controlling the alkali liquid input device to stop inputting alkali liquid, controlling the stirring device to stop stirring, and discharging the liquid in the sterilization tank. According to a second aspect of the present invention, there is provided a sterilization apparatus comprising: A sterilizing tank for carrying a sample liquid to be sterilized and an alkali liquid for sterilization; The alkali liquor input device is connected with the