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CN-117821638-B - Molecular marker and method for identifying wheat plant height and thousand seed weight

CN117821638BCN 117821638 BCN117821638 BCN 117821638BCN-117821638-B

Abstract

The invention discloses a molecular marker and a method for identifying the plant height and thousand kernel weight of wheat. The molecular marker disclosed by the invention is a nucleotide corresponding to 2184 position of SEQ ID No.1 in a sequence table in a wheat genome, and is G or A. Experiments prove that the homozygous wheat strain with G corresponding to the 2184 nucleotide of SEQ ID No.1 in the sequence table is higher than homozygous wheat with A at the site, and the thousand grain weight of the homozygous wheat with A at the site is higher than that of the homozygous wheat with G at the site.

Inventors

  • LI CHAONAN
  • JIA YUJING
  • JING RUILIAN
  • MAO XINGUO
  • WANG JINGYI
  • LI LONG

Assignees

  • 中国农业科学院作物科学研究所

Dates

Publication Date
20260505
Application Date
20231225

Claims (8)

  1. 1. Application of substances for detecting wheat molecular markers in detecting or assisting in detecting wheat plant height or grain yield; the wheat molecular marker is a nucleotide corresponding to 2184 position of SEQ ID No.1 in a sequence table in a wheat genome, and is G or A.
  2. 2. The method according to claim 1, wherein the substance for detecting the wheat molecular marker is a Primer pair named TaTIFY C-4A-Primer or a kit for detecting the wheat molecular marker; The TaTIFY C-4A-Primer consists of two single-stranded DNAs shown as SEQ ID No.4 and SEQ ID No.5 in a sequence table; The kit for detecting the wheat molecular marker comprises a Primer pair named TaTIFY C-4A-Primer-SacI, wherein TaTIFY C-4A-Primer-SacI consists of two single-stranded DNAs shown as SEQ ID No.2 and SEQ ID No.3 in a sequence table.
  3. 3. The method of claim 2, wherein the kit further comprises a restriction enzyme SacI.
  4. 4. A use according to any one of claims 1-3, characterized in that: The plant height of the wheat with the nucleotide G corresponding to the 2184 position of SEQ ID No.1 in the sequence table is larger than or is candidate to be larger than the plant height of the wheat with the nucleotide A corresponding to the 2184 position of SEQ ID No.1 in the sequence table; The grain yield of wheat having a nucleotide corresponding to position 2184 of SEQ ID No.1 in the sequence Listing is higher or alternatively higher than the grain yield of wheat having a nucleotide corresponding to position 2184 of SEQ ID No.1 in the sequence Listing.
  5. 5. A method for detecting or assisting in detecting the plant height or the grain yield of wheat, which is characterized by comprising the steps of detecting the wheat molecular marker in claim 1, and determining the plant height or the grain yield of the wheat according to the following method: The plant height of the wheat with the nucleotide G corresponding to the 2184 position of SEQ ID No.1 in the sequence table is larger than or is candidate to be larger than the plant height of the wheat with the nucleotide A corresponding to the 2184 position of SEQ ID No.1 in the sequence table; The grain yield of wheat having a nucleotide corresponding to position 2184 of SEQ ID No.1 in the sequence Listing is higher or alternatively higher than the grain yield of wheat having a nucleotide corresponding to position 2184 of SEQ ID No.1 in the sequence Listing.
  6. 6. The method according to claim 5, wherein the detection of the wheat molecular marker according to claim 1 is performed using the substance for detecting a wheat molecular marker according to any one of claims 1 to 3.
  7. 7. Any of the following applications: x1) the application of the wheat molecular marker in the wheat plant height or grain yield breeding in the claim 1; Use of X2) the wheat molecular markers of claim 1 for detecting or aiding in the detection of wheat plant height or grain yield; Use of X3) a substance according to any one of claims 1-3 for detecting molecular markers in wheat plant height or grain yield breeding; use of X4) a substance according to any one of claims 1-3 for detecting a molecular marker in the preparation of a wheat plant height or grain yield breeding product; Use of X5) a substance for detecting a molecular marker of wheat according to any one of claims 1 to 3 for the preparation of a product for detecting or aiding in the detection of wheat plant height or grain yield; x6) use of the method of claim 5 or 6 in wheat plant height or grain yield breeding; X7) detecting the substance corresponding to 2184 nucleotide of SEQ ID No.1 in the sequence table in the genome of wheat, and the application thereof in breeding wheat with excellent plant height or grain yield; x8) detecting the 2184 nucleotide corresponding to SEQ ID No.1 in the sequence table in the genome of wheat, and preparing and breeding the products of wheat with excellent plant height or grain yield.
  8. 8. A wheat plant height or seed yield breeding method comprises detecting 2184 nucleotide corresponding to SEQ ID No.1 in a sequence table in a wheat genome, and selecting wheat with 2184 nucleotide corresponding to SEQ ID No.1 in the sequence table as a parent for breeding.

Description

Molecular marker and method for identifying wheat plant height and thousand seed weight Technical Field The invention relates to a molecular marker and a method for identifying the plant height and thousand seed weight of wheat in the field of biotechnology. Background Wheat (Triticum aestinum L.) is one of three main grain crops in China, and the improvement of the wheat yield is important for guaranteeing the national grain safety. Plant height is an important agronomic character for determining crop yield, plays an important role in plant morphogenesis and yield improvement, and thousand grain weight is one of factors constituting wheat yield and directly determines the yield. Along with the development of molecular biology, molecular marker assisted breeding provides a convenient and quick method for selecting target traits of wheat, and the development and the utilization of excellent gene resources by using the molecular marker provide a basis for improving the breeding efficiency and a high-efficiency path for genetic improvement and germplasm innovation of crops. Disclosure of Invention The invention aims to solve the technical problem of how to identify the plant height and thousand seed weight of wheat. In order to solve the technical problems, the invention firstly provides application of a substance for detecting wheat molecular markers in detecting or assisting in detecting wheat plant height or grain yield; the wheat molecular marker is a nucleotide corresponding to 2184 position of SEQ ID No.1 in a sequence table in a wheat genome, and is G or A. In the application, the substance for detecting the wheat molecular marker is a Primer pair named TaTIFY C-4A-Primer or a complete reagent for detecting the wheat molecular marker; The TaTIFY C-4A-Primer consists of two single-stranded DNAs shown as SEQ ID No.4 and SEQ ID No.5 in a sequence table; The kit for detecting the wheat molecular marker comprises a Primer pair named TaTIFY C-4A-Primer-SacI, wherein TaTIFY C-4A-Primer-SacI consists of two single-stranded DNAs shown as SEQ ID No.2 and SEQ ID No.3 in a sequence table. The kit may further comprise the restriction enzyme SacI. The kit of reagents may be the TaTIFY C-4A-Primer-SacI, or may be derived from the TaTIFY11C-4A-Primer-SacI and SacI. In the application, the plant height of the homozygous wheat with G corresponding to the 2184 th nucleotide of SEQ ID No.1 in the genome is larger than or alternatively larger than the plant height of the homozygous wheat with A corresponding to the 2184 th nucleotide of SEQ ID No.1 in the genome; The grain yield of homozygous wheat whose nucleotide corresponding to position 2184 of SEQ ID No.1 in the sequence Listing is A is higher or alternatively higher than that of homozygous wheat whose nucleotide corresponding to position 2184 of SEQ ID No.1 in the sequence Listing is G. The invention also provides a method for detecting or assisting in detecting the plant height or the grain yield of wheat, which comprises the steps of detecting the wheat molecular marker and determining the plant height or the grain yield of the wheat according to the following method: the plant height of the homozygous wheat with G corresponding to the 2184 th nucleotide of SEQ ID No.1 in the sequence table is larger than or alternatively larger than the plant height of the homozygous wheat with A corresponding to the 2184 th nucleotide of SEQ ID No.1 in the sequence table; The grain yield of homozygous wheat whose nucleotide corresponding to position 2184 of SEQ ID No.1 in the sequence Listing is A is higher or alternatively higher than that of homozygous wheat whose nucleotide corresponding to position 2184 of SEQ ID No.1 in the sequence Listing is G. In the above method, the detection of the wheat molecular marker may be performed using the substance for detecting a wheat molecular marker. In the method, the detection of the wheat molecular marker by using the TaTIFY C-4A-Primer can comprise the steps of carrying out PCR amplification on wheat genome DNA by using the TaTIFY C-4A-Primer to obtain an amplification product, sequencing the amplification product, and determining the 2184 nucleotide corresponding to SEQ ID No.1 in a sequence table in the wheat genome. The reaction system for PCR amplification may be ddH 2 O12.2. Mu.L, 5 XPCR buffer 4.0. Mu.L, forward primer (10. Mu. Mol/L) and reverse primer (10. Mu. Mol/L) each 0.4. Mu. L, dNTPs (2.5 mmol/L) 1.6. Mu. L, transfastpfu enzyme (5U) 0.4. Mu.L, template DNA (20 ng/. Mu.L) 1. Mu.L. Both 5 XPCR buffer and transfastpfu U enzyme (5U) are available from Beijing full gold biotechnology Co. The reaction conditions for PCR amplification can be 95℃for 5min, 95℃for 30s,60℃for 30s,72℃for 1.5min,35 cycles, and 72℃for 10min,4℃for preservation. In the method, the detection of the wheat molecular marker by using the kit reagent can comprise the steps of carrying out PCR amplification on wheat genome DNA by using TaTIFY C-4A-Primer-SacI to