CN-117844861-B - Protein IbMYB4 related to resistance of sweet potato stem nematode disease and application thereof

Abstract

The invention relates to the technical field of biology, and discloses a protein IbMYB related to resistance of sweet potato stem nematode disease and application thereof. The invention discloses an application of IbMYB protein in improving resistance of sweet potato stem nematode disease. Experiments prove that over-expression of IbMYB.sup.4 gene in sweetpotato can enhance the resistance of sweetpotato to stem nematodes.

Inventors

• ZHAI HONG
• LIU QINGCHANG
• HE SHAOZHEN
• GAO SHAOPEI
• ZHANG HUAN
• ZHAO NING
• HU YUANFENG
• ZHAO HONGYUAN

Assignees

• 中国农业大学

Dates

Publication Date

20260505

Application Date

20240103

Claims (6)

1. Application of IbMYB4 protein in improving resistance of sweet potato stem nematode disease; the IbMYB protein is a protein of A1 or A2 as follows: A1, the amino acid sequence is protein of the amino acid sequence shown as SEQ ID No.2 in the sequence table; a2, N-terminal or/and C-terminal of A1) is linked to a protein tag.
2. 2. The method according to claim 1, wherein the IbMYB4 protein is derived from sweet potato.
3. 3. Use of a substance for increasing expression level of a gene encoding IbMYB protein of claim 1 for increasing resistance to sweetpotato stem nematode disease, wherein the substance for increasing expression level of the gene encoding IbMYB protein is a nucleic acid molecule encoding IbMYB protein of claim 1.
4. 4. The method of claim 3, wherein the IbMYB4 protein is derived from sweet potato.
5. 5. The method according to claim 4, wherein the coding sequence of the nucleic acid molecule which codes for a strand is the nucleic acid molecule of SEQ ID No.1 of the sequence Listing.
6. 6. A method for improving the resistance of sweet potatoes to stem nematodes, which is characterized by comprising the step M of enhancing, increasing or up-regulating the expression level of a gene encoding the IbMYB protein according to claim 1 to improve the resistance of sweet potatoes to stem nematodes.

Description

Protein IbMYB4 related to resistance of sweet potato stem nematode disease and application thereof Technical Field The invention relates to a protein IbMYB related to resistance of sweet potato stem nematode disease in the field of biotechnology and application thereof. Background The MYB family is one of the largest gene families in plants, and MYB family genes are widely involved in regulating and controlling biological processes of secondary metabolism, morphogenesis, response to environmental factors and hormones, disease resistance and the like of plants. In plants, the N-terminus of MYB-like transcription factors has a highly conserved MYB domain. The domain typically comprises 1-4 tandem incomplete repeat units consisting of 51-52 amino acids. The 51-52 amino acids in each repeat unit may form three alpha helices, the latter two of which may form a helix-turn-helix (HTH) structure that binds to the major groove of DNA. While the three spaced tryptophan residues contained in each repeating unit have a hydrophobic effect and serve to maintain the stability of the helix structure of HLH. In plants, MYB transcription factors play an important role in responding to abiotic stress. Overexpression of the Arabidopsis AtMYB2 and AtMYB60 genes can improve drought tolerance in Arabidopsis (Hoeren et al, 1998; cominelli et al, 2005). AtMYB68 has been shown to be involved in high temperature response reactions (Feng et al, 2004). Overexpression of the OsMYB4 gene can remarkably improve tolerance of rice plants to stress such as high salt and drought (Vannini and the like, 2004). In addition, MYB transcription factors play an important role in the response of plants to a variety of environmental factors such as hypoxia stress, hormone induction, UV-B radiation. Sweet potato stem nematode disease is caused by the parasitism of potato rot nematodes (Ditylenchus destructor). The stem nematodes have strong tolerance to low temperature and propagate throughout the year, and can continuously harm sweet potatoes in the growth period and the storage period of the sweet potatoes. Stem nematode disease can be classified into "bran-heart type", "bran-skin type" and "mixed type" according to symptoms. The common disease causes 30% -50% of yield reduction, serious even harvest failure, and in recent years, the disease is rapidly spread and aggravated year by year, and serious harm is caused to the sweet potato production, so that the sweet potato has become one of three sweet potato diseases. Therefore, the improvement of the resistance of the sweet potato stem nematode disease has important significance. Therefore, the cloning of the genes related to the resistance of the stem nematode in the sweet potato has great significance in researching the functions of the genes. The development of new genes with stress resistance and utilization thereof by means of bioengineering are effective approaches to improve stress resistance of plants. At present, the effect of MYB4 in the resistance of the stem nematode is not reported, so that cloning of the stem nematode resistance related genes in the sweet potato has very important significance in researching the functions of the genes. The development of new genes with stress resistance and utilization thereof by means of bioengineering are effective approaches to improve stress resistance of plants. Disclosure of Invention The technical problem to be solved by the invention is how to improve the resistance of sweet potato stem nematode disease. In order to solve the technical problems, the invention firstly provides IbMYB protein, a substance for improving the expression quantity of the IbMYB protein coding gene and an application of a substance for improving the content or activity of the IbMYB protein in improving the resistance of sweet potato stem nematode; The IbMYB protein is a protein of A1, A2 or A3 as follows: A1, the amino acid sequence is protein of the amino acid sequence shown as SEQ ID No.2 in the sequence table; A2, the protein which has more than 80 percent of identity with the protein shown in A1) and is related to the resistance of the sweet potato stem nematode is obtained by substituting and/or deleting and/or adding the amino acid residue in the amino acid sequence shown in SEQ ID No.2 in the sequence table; A3, N-terminal or/and C-terminal of A1) or A2). In the above application, SEQ ID No.2 of the sequence Listing consists of 239 amino acid residues. In the above applications, identity refers to the identity of amino acid sequences. The identity of amino acid sequences can be determined using homology search sites on the internet, such as BLAST web pages of the NCBI homepage website. For example, in advanced BLAST2.1, by using blastp as a program, expect values are set to 10, all filters are set to OFF, BLOSUM62 is used as Matrix, gap existence cost, per residue gap cost and Lambda ratio are set to 11,1 and 0.85 (default values), respectively, and identity of a pa