CN-117860828-B - Hair-growing white tea nanoparticle/Ca2+Preparation method and application of composite system

Abstract

The invention relates to the fields of medicines and cosmetics, in particular to a white tea nanoparticle/Ca 2+ composite system with a hair growing function, and a preparation method and application thereof. The invention discloses a preparation method of a white tea nanoparticle/Ca 2+ composite system, which comprises the following steps of grinding white tea, mixing tea powder with water, heating and extracting, centrifuging the obtained extracting solution for the first time, centrifuging the supernatant obtained by the first time, performing ultrasonic cleaning on the precipitate obtained by the second centrifugation, freeze-drying, preparing white tea nanoparticle dry powder into white tea nanoparticle suspension, dripping CaCl 2 solution, centrifuging, performing ultrasonic cleaning on the precipitate, and freeze-drying to obtain the white tea nanoparticle/Ca 2+ composite system. The germinal white tea nanoparticle/Ca 2+ composite system can be used for preparing medicines or reagents for promoting hair growth.

Inventors

• CHU QIANG
• ZHOU SU
• CHEN MING
• CHEN PING
• GUO HAOWEI
• FENG XINYU
• PAN YANI

Assignees

• 浙江大学

Dates

Publication Date

20260508

Application Date

20240105

Claims (5)

1. 1. The preparation method of the white tea nanoparticle/Ca 2+ composite system for growing hair is characterized by comprising the following steps of: (1) Grinding the white tea to obtain tea powder; (2) Mixing the tea powder obtained in the step (1) with water according to a feed liquid ratio of 1g to 25-35 mL, heating and leaching the obtained mixture at 90-100 ℃ for 35-40 min, and performing suction filtration to obtain an extracting solution; (3) Centrifuging the extracting solution obtained in the step (2) for 10+/-2 min at 4000-5000 r/min for the first time, and centrifuging the supernatant obtained by the first time at 6000-7000 r/min for 10+/-2 min for the second time; (4) Re-suspending the white tea nano-particle dry powder obtained in the step (3) in pure water to prepare 5+/-0.5 mg/ml white tea nano-particle suspension; Taking 6ml of white tea nano particle suspension, magnetically stirring for 3+/-1 min, adding 1-2 ml of CaCl 2 solution with the concentration of 5+/-0.5 mg/ml, stirring for 30+/-5 min, centrifuging for 10+/-2 min at 6000-7000 rpm, taking the precipitate, ultrasonically cleaning, and freeze-drying to obtain the white tea nano particle/Ca 2+ composite system.
2. 2. The method for preparing the hair tonic white tea nanoparticle/Ca 2+ composite system according to claim 1, wherein in the step (1), the particle size of the tea powder is 30-50 mesh.
3. 3. The method for preparing the germinal white tea nanoparticle/Ca 2+ composite system according to claim 2, which is characterized in that: the ultrasonic cleaning is to add pure water which is not precipitated into the precipitate, repeatedly blow the precipitate while ultrasonic, continuously centrifuging for 10+/-2 min at the speed of 6000-7000 rpm for 5+/-1 min, and repeating the ultrasonic cleaning operation on the obtained precipitate again.
4. 4. The germinal white tea nanoparticle/Ca 2+ composite system prepared by the method according to any one of claims 1-3.
5. 5. The application of the white tea nanoparticle/Ca 2+ composite system for promoting hair growth prepared by the method according to any one of claims 1-3 in the preparation of medicines or cosmetics for promoting hair growth.

Description

Preparation method and application of germinal white tea nanoparticle/Ca 2+ composite system Technical Field The invention relates to the fields of medicines and cosmetics, in particular to a white tea nanoparticle/Ca 2+ composite system with a hair growing function, and a preparation method and application thereof. Background The scalp ecosystem has dense hair, abundant glands and complex structure, wherein hair follicles positioned at the root of the hair for fixing the hair are important appendages of the scalp. Mature hair follicles are complex micro-organs, the growth cycle of which is strictly regulated, and in the postnatal development process, the hair follicles undergo successive stages of anagen, catagen and telogen, and are cycled back and forth, and whether the process can be normally performed determines whether the hair can smoothly perform periodical growth. Therefore, anchoring the hair follicle microstructure to develop a hair growth promoting study is critical in the hair care and hair growth field. However, products anchoring and penetrating hair follicles are lacking in the market at present, and most hair care products contain certain toxic chemical substances, so that the long-term use of the hair care products can have a certain negative effect on the health of human bodies. Therefore, aiming at the close relation between hair follicles and hair, the development of safe and effective hair washing and caring products capable of penetrating into the hair follicles is of great significance. With the development of nanotechnology, the application of nanomaterials has attracted attention, and the encapsulation of substances into nanoparticles is expected to improve the bioavailability of products for topical application. The nanoparticles cannot cross the intact skin barrier but can penetrate into the hair follicle and release the active substance therein. Studies show that the medium-sized (about 640 nm) particles have the strongest ability to enter the hair follicle, and different sites in the hair follicle can be selectively targeted by changing the particle size. Thus, nanotechnology is expected to provide a new strategy for the development of germinal products that anchor the hair follicle structure. In recent years, with the intensive research on health care efficacy of tea leaves, white tea is gradually coming into the public view due to its excellent nutritional ingredients and active functions. White tea is a tea made through withering and drying processes, and forms a specific quality style along with a series of physical and chemical component changes in the processing process. A large number of research results show that the white tea contains functional substances such as polyphenol, caffeine, amino acid and the like which are more abundant than other tea seeds, has the functions of resisting oxidation, resisting bacteria, diminishing inflammation, repairing skin barriers, protecting damaged cells and the like, and has important significance for regulating and controlling the microecology of scalp. The prior researches prove that the active ingredients such as EGCG in the white tea can reduce the concentration of cell inflammatory factors, and can play a certain role in relieving inflammatory reactions of blood vessels, intestinal tracts, nerves and the like in animal bodies. However, the single white tea component has poor curative effect, and a composite system is also required to be constructed to enhance the curative effect. Studies have shown that Ca 2+ is a key factor for regulating cell proliferation and maturation and promoting barrier function of epidermal lipids, plays an important role in promoting wound healing, epidermis regeneration and dermis reconstruction, and meanwhile, ca 2+ can promote the scavenging capacity of EGCG on free radicals and improve the antioxidant function and anti-inflammatory effect of EGCG. Disclosure of Invention The invention aims to provide a white tea nanoparticle/Ca 2+ composite system with a hair growing function, and a preparation method and application thereof. In order to solve the technical problems, the invention provides a preparation method of a germinal white tea nanoparticle/Ca 2+ composite system, which comprises the following steps: (1) Grinding white tea (grinding in a high-speed pulverizer, and sieving) to obtain tea powder; (2) Mixing the tea powder obtained in the step (1) with water according to a feed liquid ratio of 1g to 25-35 mL, heating and leaching the obtained mixture at 90-100 ℃ for 35-40 min, and performing suction filtration to obtain an extracting solution; Preferably, the ratio of 1g to 30mL is adopted, and the leaching is carried out for 40min under the heating of 100 ℃; (3) Gradient centrifugation, namely centrifuging the extracting solution obtained in the step (2) for 10+/-2 min at 4000-5000 r/min for the first time, taking supernatant obtained by the first centrifugation for secondary centrifugation at