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CN-117865338-B - Full biological treatment method for high-concentration pyridine wastewater

CN117865338BCN 117865338 BCN117865338 BCN 117865338BCN-117865338-B

Abstract

The invention discloses a full biological treatment method for high-concentration pyridine wastewater, and belongs to the field of biological treatment of high-concentration pyridine wastewater. The method has the advantages that the process is simple to operate, the reaction is efficient, the pyridine with the total biological degradation efficiency of less than 4000mg/L can be completely biodegraded, the pyridine degradation efficiency is higher than 99%, and the method can treat high-concentration pyridine wastewater by a full biological method, and has good effect, low cost and no secondary pollution. The method of the invention is to strengthen the upgrading of the common biological method and widen the application range of biological treatment of pyridine wastewater.

Inventors

  • GAO FENG
  • SHAO RUYING
  • ZHAO ZHENHUA
  • XIA JIAO

Assignees

  • 江苏蓝必盛化工环保股份有限公司

Dates

Publication Date
20260508
Application Date
20240109

Claims (6)

  1. 1. The full biological treatment method of the high-concentration pyridine wastewater is characterized by comprising the following steps of: Step one, screening and domesticating pyridine strains; 1.1, taking activated sludge of a pyridine-containing wastewater treatment plant, 1-1.5g/L glucose, tap water, pH value of 7-8, and carrying out aeration culture for 1-2d at 25-35 ℃ in a strain expansion culture tank, wherein DO is 2-4 mg/L; 1.2, under the condition of unchanged culture conditions, gradually changing the ratio of glucose water distribution to pyridine wastewater until the final pyridine concentration of the water inlet is half of the design target concentration of the scheme; 1.3, measuring COD, changing half of water every 48h after stabilization, and maintaining nutrient substances required by microorganisms in the activated sludge Step two, optimizing and culturing strains; Step three, biological treatment of pyridine wastewater: 3.1, introducing pyridine wastewater with the pH value adjusted to 7-8 into a biological treatment tank, controlling the temperature to 25-35 ℃, controlling the reaction time and dissolved oxygen, adding 10% pyridine compound bacteria liquid into the biological treatment tank, and immobilizing pyridine-specific degrading bacteria on polyurethane filler; 3.2, when the pyridine concentration of the water inlet is less than 1500 mg/L or the COD is less than 2000 mg/L, adding 500 mg/L COD equivalent glucose as a carbon source; 3.3, carrying out gradient negative extraction according to the pyridine concentration of 500-1000 mg/L or COD of 1000-1500 mg/L, wherein the COD is preferential, and each batch of water is changed by 50-60% until the water inflow load is the target water inflow pyridine concentration, wherein the full negative water inflow pyridine concentration can reach 3000-4000 mg/L; 3.4, hydraulic retention time HRT is: 500-2000 mg/L of pyridine, and 24 hours of HRT; 2000-3000 mg/L of pyridine, and 48 hours of HRT; inlet pyridine 4000 mg/L, HRT 72h, and adding 2g/L glucose as co-metabolism carbon source; 3.5, a treatment process: adopting an aerobic biochemical process, wherein the air volume is in the range of 4.5-5.0L/h, and the aerobic reaction tank is inoculated with 10% of pyridine compound bacteria liquid and 70% of polyurethane filler; the domestication target concentration of the special degrading bacteria is 2 parts, namely the pyridine concentration of 1/2 target wastewater and the pyridine concentration of target wastewater, so that special compound bacteria capable of treating low-concentration pyridine and high-concentration pyridine can be obtained by screening as far as possible.
  2. 2. The full biological treatment method of high-concentration pyridine wastewater according to claim 1, wherein the second step is specifically: 2.1, taking out the activated sludge of the wastewater in the culture expansion tank after the step one, preparing a suspension, adding distilled water, shaking uniformly, centrifuging the suspension for 10min at a rotating speed of 3000r/min, and taking a supernatant; 2.2, taking a loop of supernatant liquid by an inoculating loop, inoculating the loop of supernatant liquid into a solid culture medium, culturing the solution for 48 to 72 hours at room temperature, and observing that obvious strain growth exists on the surface of a culture dish; 2.3, picking microorganisms in colonies with different shapes in the solid culture medium by using an inoculating loop, and culturing for 48-72 hours in the solid culture medium with 1/2 target pyridine concentration of wastewater and the solid culture medium with the target pyridine concentration of wastewater respectively by using a plate streaking method at the temperature of 25-30 ℃; 2.4, repeating the step 2.3 as required until only a single colony exists in the solid culture medium, namely finishing the screening and purification of the microbial strains; 2.5, picking colonies with different shapes in the solid culture medium, carrying out shake cultivation for 48-72 hours at the temperature of 25-30 ℃ in the liquid culture medium, and preparing the bacterial strains obtained on the flat plates with different concentrations into pyridine compound bacterial liquid after optimized cultivation.
  3. 3. The full biological treatment method of high-concentration pyridine wastewater according to claim 1, wherein in the biological treatment process of the pyridine wastewater in the step 3.1-3.3, the pH of the inlet water is 7-8, and the optimal pyridine inlet water concentration is 500-3000 mg/L.
  4. 4. The full biological treatment method of high-concentration pyridine wastewater according to claim 1, wherein the implementation mode of pyridine-specific degradation bacteria and polyurethane filler immobilization is that pyridine-specific degradation bacteria are added into a polyurethane filler reaction tank filled with 70% according to the addition amount of 10% in 2-3 batches, the retention time is controlled to be 24-72h, indexes of COD, TOC indexes and ammonia nitrogen are measured by sampling, the degradation rate is more than 90% and stable for 5-10d, the pyridine concentration of water is gradually increased by a pyridine concentration gradient of 500-1000 mg/L, and each batch of water is changed by 50-60% until the water inlet load is the target pyridine concentration.
  5. 5. The full biological treatment method of high-concentration pyridine waste water according to claim 1, wherein glucose is used as a co-metabolism carbon source, the corresponding enzyme involved in the metabolism of a substrate is induced to synthesize or secrete while providing energy for the growth of microorganisms, and cofactors are produced in metabolic pathways as electron donors, and the co-metabolites can also participate in the degradation process of the nondegradable compounds.
  6. 6. The full biological treatment method of high-concentration pyridine wastewater according to claim 1, wherein the implementation mode of pyridine-specific degradation bacteria and polyurethane filler immobilization is that pyridine-specific degradation bacteria are added into a polyurethane filler reaction tank filled with 70% according to the addition amount of 10% in 2-3 batches, the retention time is controlled to be 24-72h, pyridine, TOC indexes and ammonia nitrogen indexes are measured by sampling, the degradation rate is higher than 90% and stabilized for 5-10d, the concentration of pyridine in water is gradually increased by a pyridine concentration gradient of 500-1000 mg/L, and each batch of water is changed by 50-60% until the water inlet load is the target pyridine concentration in water.

Description

Full biological treatment method for high-concentration pyridine wastewater Technical Field The invention belongs to the field of biological treatment of high-concentration pyridine wastewater, and particularly relates to a biological treatment method of pyridine wastewater. Background Pyridine (C 5H5 N) is an organic compound, is a six-membered heterocyclic compound containing one nitrogen heteroatom, and is industrially used as a denaturant, a dyeing auxiliary and a raw material for synthesizing a series of products (including medicines, disinfectants, dyes and the like). The current pyridine treatment modes include adsorption method, rectification method and incineration method. The adsorption method, distillation method and equipment are complex, the treatment cost is high, and the incineration method can produce the secondary pollution of waste gas. Therefore, the developed specific strain screening and application method for the pyridine wastewater can treat the high-concentration pyridine wastewater with high efficiency and low cost, and has great application and popularization values. Therefore, the patent discloses a full biological treatment method for high-concentration pyridine wastewater, which can rapidly screen and obtain pyridine-specific degradation bacteria at low cost, and glucose is added for co-metabolism in the aerobic degradation process of pyridine, so that the degradation rate of the pyridine can be improved, and the degradation time of the pyridine can be shortened. The method is used for upgrading and strengthening the common biological method and widening the application range of biologically treating the pyridine wastewater. Disclosure of Invention Aiming at the problems in the prior art, the invention discloses a full biological treatment method for high-concentration pyridine wastewater, which has simple process operation and high reaction efficiency, can lead the wastewater with the concentration of the pyridine to be lower than 4000mg/L, can carry out professional biodegradation, and has the pyridine degradation efficiency higher than 99 percent. The invention is realized in the following way: the full biological treatment method of the high-concentration pyridine wastewater is characterized by comprising the following steps of: Step one, screening and domesticating pyridine strains: 1.1, taking activated sludge of a pyridine-containing wastewater treatment plant, 1-1.5g/L glucose, tap water, pH value of 7-8, and performing aeration culture for 1-2d at 25-35 ℃ in a strain expansion culture tank, wherein DO is 2-4mg/L; 1.2, under the condition of unchanged culture conditions, gradually changing the ratio of glucose water distribution to pyridine wastewater until the final pyridine concentration of the water inlet is half of the design target concentration of the scheme; 1.3, measuring COD, changing water by half every 48H after stabilization, and maintaining nutrient substances required by microorganisms in the activated sludge. Step two, optimizing culture of strains: 2.1, taking out the activated sludge of the wastewater in the culture expansion tank after the step one, preparing a suspension, adding distilled water, shaking uniformly, centrifuging the suspension for 10min at a rotating speed of 3000r/min, and taking a supernatant; 2.2, taking a loop of supernatant liquid by an inoculating loop, inoculating the loop of supernatant liquid into a solid culture medium, culturing the solution for 48 to 72 hours at room temperature, and observing that obvious strain growth exists on the surface of a culture dish; 2.3, picking microorganisms in colonies with different shapes in the solid culture medium by using an inoculating loop, and culturing for 48-72 hours in the solid culture medium with 1/2 target pyridine concentration of wastewater and the solid culture medium with the target pyridine concentration of wastewater respectively by using a plate streaking method at the temperature of 25-30 ℃; 2.4, repeating the step 2.3 as required until only a single colony exists in the solid culture medium, namely finishing the screening and purification of the microbial strains; 2.5, picking colonies with different shapes in the solid culture medium, carrying out shake cultivation for 48-72 hours at the temperature of 25-30 ℃ in the liquid culture medium, and preparing the bacterial strains obtained on the flat plates with different concentrations into pyridine compound bacterial liquid after optimized cultivation. Step three, biological treatment of pyridine wastewater: 3.1, introducing pyridine wastewater with the pH value adjusted to 7-8 into a biological treatment tank, controlling the temperature to 25-35 ℃, controlling the reaction time and dissolved oxygen, adding 10% pyridine compound bacteria liquid into the biological treatment tank, and immobilizing pyridine-specific degrading bacteria on polyurethane filler; 3.2, when the pyridine concentration of the water inlet is less than