CN-117882819-B - Oil-soluble beet red pigment and preparation method and application thereof

Abstract

The invention belongs to the field of natural pigment modification, and particularly relates to an oil-soluble beet red pigment, and a preparation method and application thereof. The invention adds the aqueous solution containing betalain and phosphatide into the aqueous solution containing water-soluble protein and/or water-soluble polypeptide, dries to obtain oil-soluble betalain composite powder, then carries out low-temperature superfine grinding on the powder to obtain oil-soluble betalain superfine powder, adds the oil-soluble betalain superfine powder into vegetable oil, and carries out cyclic treatment of a ball mill-colloid mill to obtain the oil-soluble betalain. The oil-soluble betalain prepared by the invention does not need chemical modification, does not add components such as synthetic emulsifier, and the like, and the used materials are natural and safe, so that the light stability and the heat stability of the betalain are improved, the application range of the betalain is expanded, and the oil-soluble betalain can replace industrial synthetic oil-soluble pigment.

Inventors

• XU XIAOSONG
• JIN ZIHENG
• WEN YANJUN
• LI LINZHENG
• LI HONGLONG
• WANG DI
• YU CHUNFENG

Assignees

• 河南中大恒源生物科技股份有限公司

Dates

Publication Date

20260512

Application Date

20240111

Claims (10)

1. 1. The preparation method of the oil-soluble beet red pigment is characterized by comprising the following steps: (1) Adding the aqueous solution containing the betalain and the phospholipid into the aqueous solution containing the water-soluble protein and/or the water-soluble polypeptide to obtain a betalain-phospholipid-protein/polypeptide mixed solution; (2) Drying the betalain-phospholipid-protein/polypeptide mixed solution prepared in the step (1) to obtain oil-soluble betalain composite powder; (3) Carrying out low-temperature superfine grinding on the oil-soluble betalain composite powder prepared in the step (2) to obtain oil-soluble betalain superfine powder; (4) Adding the oil-soluble betalain ultra-fine powder prepared in the step (3) into vegetable oil, and grinding and dispersing to obtain the oil-soluble betalain; The phospholipid in the step (1) is at least one of lecithin, modified soybean phospholipid and enzymatic soybean phospholipid; The water-soluble protein in the step (1) is at least one of collagen, soy protein isolate and sodium caseinate; the polypeptide in the step (1) is soybean oligopeptide; The mass ratio of the betalain to the phospholipid in the step (1) is 1 (0.05-30.0); the mass fraction of the water solution containing the water-soluble protein and/or the water-soluble polypeptide in the step (1) is 5-45%; in the betalain-phospholipid-protein/polypeptide mixed solution in the step (1), the mass ratio of the total amount of water-soluble protein and water-soluble polypeptide to betalain is (0.01-13.0): 1.
2. 2. The method for preparing the oil-soluble betalain according to claim 1, wherein: and (3) slowly adding the aqueous solution containing the betalain and the phospholipid in the step (1) into the aqueous solution containing the water-soluble protein and/or the water-soluble polypeptide, and shearing for uniformly mixing to obtain the betalain-phospholipid-protein/polypeptide mixed solution.
3. 3. The method for preparing the oil-soluble betalain according to claim 1, wherein: the drying in the step (2) is spray drying.
4. 4. The method for preparing the oil-soluble betalain according to claim 1, wherein: the vegetable oil in the step (4) is at least one of olive oil, corn oil, sunflower seed oil, safflower seed oil and soybean oil.
5. 5. The method for preparing the oil-soluble betalain according to claim 1, wherein: the aqueous solution containing betalain and phospholipid in the step (1) is prepared by the following method: Mixing beet red pigment and phospholipid, and dissolving in water to obtain uniform solution, i.e. water solution containing beet red pigment and phospholipid.
6. 6. The method for preparing the oil-soluble betalain according to claim 1, wherein: the low temperature in the step (3) is 0-10 ℃.
7. 7. The method for preparing the oil-soluble betalain according to claim 1, wherein: the granularity of the oil-soluble beet red ultra-fine powder in the step (3) is 100-300 meshes.
8. 8. The method for preparing the oil-soluble betalain according to claim 1, wherein: the mass fraction of the beet red ultra-fine powder in the step (4) in the vegetable oil is 10-50%.
9. 9. The method for preparing the oil-soluble betalain according to claim 1, wherein: the grinding and dispersing in the step (4) are the circulating treatment of a ball mill-colloid mill.
10. 10. The method for preparing the oil-soluble betalain according to claim 1, wherein: the grinding and dispersing treatment in the step (4) is carried out until D90 of betalains is less than 0.5 mu m.

Description

Oil-soluble beet red pigment and preparation method and application thereof Technical Field The invention belongs to the field of natural pigment modification, and particularly relates to an oil-soluble beet red pigment, and a preparation method and application thereof. Background Beet red (beet root red) is also called as beet root red, and is a natural pigment prepared from edible red beet by leaching, separating, concentrating and drying, and the main components are beet anthocyanin and betaxanthin, and the beet red pigment is red purple to dark purple liquid, block, powder or paste, has abnormal odor, is easy to dissolve in water, is difficult to dissolve in absolute ethyl alcohol, propylene glycol and acetic acid, and is insoluble in organic solvents such as diethyl ether, acetone, chloroform, benzene, glycerol, grease and the like. The aqueous solution presents red to mauve, has bright color and good dyeing property, but has poor heat resistance, and the degradation speed increases with the temperature. The use of concentrates or dehydrated red beet powder of red beet juice as food colorants has been approved as early as 1960 in the united states and the "exempt safety certification" approval list has been established to date by the united nations FAO/WHO united food additive expert committee 1978 again prescribing its tentative ADI as "no need to prescribe". The betanin belongs to inert substances in pharmacology and is as safe as the edible beet. The food additive is used in fruit beverage, fruit juice beverage, soft drink, beverage, candy, cake with color, red and green silk, can, concentrated fruit juice, green plum, ice cream, sweet jelly, waffle biscuit, sandwich layer, etc. in proper amount. Because betalains are easily soluble in water and insoluble in grease, the application range of the betalains is greatly limited, and based on the technical staff, more researches for improving the oil solubility of the betalains are carried out, wherein: Chinese patent CN201911001462.4 discloses candida vista and application thereof in preparing oil-soluble betalain, the biosurfactant produced by candida vista fermentation is uniformly mixed with grease and betalain to prepare the oil-soluble betalain, the betalain is uniformly distributed, no precipitation occurs, but the cost of the strain used is too high, large-scale mass production is difficult to realize, and the biosurfactant lacks related standards. Chinese patent CN201711092553.4 discloses a process for emulsifying betalain, which comprises mixing betalain powder with oil phase dispersion liquid, grinding, adding emulsifying agent, vacuum emulsifying to obtain oil-soluble betalain, emulsifying and dispersing betalain particles by emulsifying agent, but betalain has no lipophilicity, and solid-liquid interface potential energy formed by betalain and grease is difficult to maintain stable by emulsifying agent, delamination and precipitation are easy to occur, and product quality and use are affected. Chinese patent CN202210880227.4 discloses a preparation method of modified betalain, which is characterized in that betalain is reacted with carboxylic acid compound to obtain modified betalain, so that the problem of hydrophilic and oleophobic betalain is solved, and the modified betalain can be applied to an oil-soluble system. However, the method requires a large amount of toxic solvents, which limits the application of betalains in the food field. Although the technology can improve the problems of hydrophilicity and oleophobicity of beet red pigment to a certain extent, certain defects and disadvantages still exist, such as complex operation of a large number of processes, high cost, uncertainty of edible safety in part of the technology, low use stability of the final product and the like, and the market demand cannot be met. Therefore, there is a need to provide a novel oil-soluble betalain and a preparation method thereof to overcome the above-mentioned drawbacks and disadvantages. Disclosure of Invention In order to overcome the defects and the shortcomings of the prior art, the primary aim of the invention is to provide a preparation method of oil-soluble betalain, which does not use organic solvents, does not need to chemically modify betalain, does not add emulsifying agents of synthetic sources, is natural and safe in materials, can uniformly and stably disperse betalain in grease, and ensures that the application range of betalain is expanded from water solubility to oil solubility, and the stability of betalain is also improved. The invention also aims to provide the oil-soluble betalain prepared by the preparation method. It is a further object of the present invention to provide the use of the oil-soluble betalains described above. The aim of the invention is achieved by the following technical scheme: a preparation method of oil-soluble beet red pigment comprises the following steps: (1) Adding the aqueous solution containing the betal