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CN-117925693-B - GmTPS1 protein and application of encoding gene thereof in improving strain type characters and yield of soybeans

CN117925693BCN 117925693 BCN117925693 BCN 117925693BCN-117925693-B

Abstract

The invention discloses GmTPS protein and application of a coding gene thereof in improving soybean plant type characters and yield. The invention belongs to the technical field of biology, and relates to GmTPS protein and application of a coding gene thereof in improving soybean plant type characters and yield. The invention provides an application of GmTPS protein or a substance for regulating and controlling the expression of a gene or a substance for regulating and controlling the activity or content of the gene in regulating and controlling plant types, wherein GmTPS protein is SEQ ID No.3. The invention uses CRISPR/Cas9 mediated gene editing technology to carry out site-directed mutation or knockout of specific targets on soybean strain type coding genes GmTPS, provides a new material for soybean variety breeding of improved soybean strain type, and has positive effects for accelerating the breeding of improved strain type soybean varieties.

Inventors

  • CHEN LI
  • HOU WENSHENG
  • CAI YUPENG

Assignees

  • 中国农业科学院作物科学研究所

Dates

Publication Date
20260505
Application Date
20240119

Claims (10)

  1. 1. Application of a substance for knocking out GmTPS protein coding genes in regulation of plant types, wherein the genes code for GmTPS protein, and GmTPS protein is protein with an amino acid sequence shown as SEQ ID No. 3; The regulation of plant types is to increase plant node number, increase single plant grain number and reduce branch number; The plant is soybean.
  2. 2. Application of substances for reducing GmTPS protein content in regulating plant type, wherein GmTPS protein is protein with an amino acid sequence shown as SEQ ID No. 3; The regulation of plant types is to increase plant node number, increase single plant grain number and reduce branch number; The plant is soybean.
  3. 3. The method according to claim 1 or 2, wherein the GmTPS protein is derived from soybean.
  4. 4. The method according to claim 1 or 2, wherein the substance is a biological material associated with the protein, the biological material being any of the following: B1 A nucleic acid molecule which inhibits or reduces or silences the expression of a gene encoding the GmTPS a protein as set forth in claim 1 or 2; b2 An expression cassette comprising the nucleic acid molecule of B1); B3 A recombinant vector comprising the nucleic acid molecule of B1) or a recombinant vector comprising the expression cassette of B2); B4 A recombinant microorganism comprising the nucleic acid molecule of B1), or a recombinant microorganism comprising the expression cassette of B2), or a recombinant microorganism comprising the recombinant vector of B3); b5 A transgenic plant cell line comprising the nucleic acid molecule of B1) or a transgenic plant cell line comprising the expression cassette of B2); b6 A transgenic plant tissue comprising the nucleic acid molecule of B1) or a transgenic plant tissue comprising the expression cassette of B2); B7 A transgenic plant organ comprising the nucleic acid molecule of B1) or a transgenic plant organ comprising the expression cassette of B2).
  5. 5. The use according to claim 4, characterized in that: the coding gene of GmTPS protein is any one of the following nucleic acid molecules: b11 A cDNA molecule whose coding sequence is SEQ ID No.2, B12 A DNA molecule of SEQ ID No. 1; B1 The nucleic acid molecule is any one of the following: b21 Targeting the gRNA of the GmTPS protein-encoding gene of claim 1 or 2; B22 A DNA molecule which expresses the gRNA of B21).
  6. 6. A method for changing soybean plant type is characterized by comprising the steps of reducing or lowering the content of GmTPS protein in claim 1 or 2 in target soybean or/and the expression level of GmTPS protein coding gene in claim 1 or 2 to change soybean plant type, wherein the soybean plant type is changed by increasing the plant node number of soybean, increasing the single plant number and reducing the branch number.
  7. 7. The method of claim 6, wherein the down-regulating or reducing the amount of GmTPS protein of claim 1 or 2 in the soybean of interest, or/and the expression level of GmTPS protein-encoding gene of claim 1 or 2, comprises knocking out the GmTPS protein-encoding gene of the soybean genome of interest with CRISPR/Cas9 system.
  8. 8. The method of claim 7, wherein the CRISPR/Cas9 system comprises a vector expressing sgRNA targeting the coding gene of GmTPS proteins.
  9. 9. The method according to claim 7 or 8, wherein the soybean strain is altered by mutating the gene encoding the GmTPS protein, the mutation being any one of the following: 1) Deletion of nucleotides 296-299 of SEQ ID No.1, while keeping the other nucleotides of SEQ ID No.1 unchanged; 2) A nucleotide T is added between nucleotide 298 and nucleotide 299 of SEQ ID No.1, and other nucleotide residues of SEQ ID No.1 are kept unchanged.
  10. 10. Use of a substance according to any one of claims 1-4 in soybean breeding; the purpose of the breeding is to breed soybean varieties with increased plant node number, increased single plant grain number and reduced branch number.

Description

GmTPS1 protein and application of encoding gene thereof in improving strain type characters and yield of soybeans Technical Field The invention belongs to the technical field of biology, and relates to GmTPS protein and application of a coding gene thereof in improving soybean plant type characters and yield. Background The soybean is used as an important grain and oil feeding crop, and plays a role in guaranteeing the grain safety and agricultural product trade in China. With the continuous improvement of the living standard of people, the demands for vegetable oil and feed protein are rapidly increased, and the problem of insufficient soybean production capacity in China is also more and more prominent. Compared with the world soybean major countries such as the United states, brazil, argentina and the like, the soybean yield in China is lower, the daily production and living needs can not be met, and the import amount of the soybean is increased year by year. Under the realistic condition that the soybean planting area in China is limited, how to quickly and effectively improve the soybean variety and the unit yield level of soybeans by means of modern biological breeding technology is an important production problem and a breeding technology bottleneck to be broken through. The plant type of the crops plays a decisive role in the morphological establishment of single plants and groups of the crops, and is an important factor for influencing the yield of the plants, the production level of the crops and the economic benefit. Crop plant types include plant height, branching (tillering), leaf shape, spike shape (pod habit), and the like. The plant type domestication or improvement of crops plays a vital role in realizing the important breakthrough of crop yield. However, the research on soybean plant type regulation mechanism and soybean plant type regulation key genes is still in an exploration stage at present, the related research reports are less, and the molecular mechanism, key genes and action network affecting soybean plant type regulation are not clear and definite. Particularly, under the condition of field production, which plant type is more favorable for improving the unit yield level of soybean due to the limitation of research materials, and the related research is lacking. Therefore, more plant type regulation genes are further excavated, the method has important theoretical value for finding excellent soybean plant type regulation genes and cultivating high-yield ideal plant types, and through creation of specific materials, the ideal soybean plant types can be systematically evaluated and optimized under production conditions, so that the method has important practical application value for finally realizing breeding application and improving soybean yield. Disclosure of Invention The invention aims to solve the technical problem of improving the soybean plant type and obtaining the target plant which is favorable for improving the unit yield level of soybean. In order to solve the technical problems, the invention firstly provides GmTPS application of a protein or an expression substance of a regulatory gene or a substance for regulating activity or content of the GmTPS protein in regulating plant type, wherein the gene codes for the GmTPS protein, and the GmTPS protein is any one of the following proteins: (a1) A protein with an amino acid sequence shown as SEQ ID No. 3; (a2) A protein which is obtained by substituting and/or deleting and/or adding an amino acid residue in the amino acid sequence shown in SEQ ID No.3, has more than 80 percent of identity with the protein shown in a 1) and has the function of regulating plant types, (A3) A fusion protein obtained by ligating a tag to the end of the protein of (a 1) or (a 2). In the above application, the GmTPS protein is derived from soybean. In the above application, the substance for regulating the expression of the gene or the substance for regulating the activity or content of GmTPS protein is a biological material related to the protein, and the biological material is any one of the following materials: B1 A nucleic acid molecule encoding said GmTPS protein; B2 A nucleic acid molecule that inhibits or reduces or silences the expression of a gene encoding the GmTPS protein; b3 An expression cassette containing the nucleic acid molecule of B1) and/or B2); b4 A recombinant vector comprising the nucleic acid molecule of B1) and/or B2) or a recombinant vector comprising the expression cassette of B3); B5 A recombinant microorganism comprising the nucleic acid molecule of B1) and/or B2), or a recombinant microorganism comprising the expression cassette of B3), or a recombinant microorganism comprising the recombinant vector of B4); B6 A transgenic plant cell line comprising the nucleic acid molecule of B1) and/or B2) or a transgenic plant cell line comprising the expression cassette of B3); B7 A) transgenic plant tissue containing the nuc