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CN-118000422-B - Composite probiotic capsule and preparation method thereof

CN118000422BCN 118000422 BCN118000422 BCN 118000422BCN-118000422-B

Abstract

The invention belongs to the technical field of microcapsules, and particularly relates to a preparation method of a composite probiotic capsule, which comprises the following steps of uniformly mixing probiotic bacterial liquid, fructo-oligosaccharide, tea polyphenol, grape polyphenol, mannooligosaccharide, isomaltulose alcohol and an emulsifier to obtain a core material, uniformly mixing glycerol and distilled water, heating to 60-80 ℃, sequentially adding modified gelatin, hydroxypropyl starch and chitosan, stirring for 10-30min, sterilizing, cooling to room temperature to obtain a capsule wall material, mixing the core material and the capsule wall material, carrying out high-pressure homogenization treatment, and then carrying out spray drying, wherein the modified gelatin is prepared from succinic acid derivatives and gelatin through acylation reaction. The composite probiotic capsule provided by the invention has excellent storage stability, oxidation resistance and gastric juice erosion resistance, and also has better enteric solubility and bioavailability.

Inventors

  • ZHAO CHONG
  • ZHAO GUODA
  • Kou Dongliang
  • CAI YI

Assignees

  • 杭州栎康生物科技有限公司

Dates

Publication Date
20260505
Application Date
20240326

Claims (6)

  1. 1. The composite probiotic capsule is characterized by comprising a core material and a capsule wall material, wherein the mass ratio of the core material to the capsule wall material is 1-3:5-9, the core material comprises, by weight, 20-40 parts of probiotic bacteria liquid, 5-8 parts of fructo-oligosaccharide, 2-4 parts of tea polyphenol, 2-4 parts of grape polyphenol, 5-8 parts of mannooligosaccharide, 3-7 parts of isomaltitol and 1-3 parts of emulsifying agent, the probiotic bacteria liquid is formed by mixing equal weight of bifidobacterium bacteria liquid and lactobacillus acidophilus bacteria liquid, and the capsule wall material comprises, by weight, 40-60 parts of modified gelatin, 50-70 parts of distilled water, 5-10 parts of glycerol, 20-30 parts of hydroxypropyl starch and 15-20 parts of chitosan; the preparation method of the modified gelatin comprises the following steps: S1, dispersing (2R, 3R) - (-) -epoxy succinic acid in 2-methyltetrahydrofuran under the stirring condition, heating to 60-70 ℃, sequentially adding N-ethylmorpholine and 4-methyl-1-amyl alcohol, stirring for reacting for 5-8h, cooling to room temperature, carrying out solid-liquid separation, taking liquid, and carrying out reduced pressure distillation, washing and drying to obtain an intermediate, wherein the structural formula of the intermediate is as follows: ; S2, dispersing 3, 5-di-tert-butyl-4-hydroxybenzoic acid in 2-methyltetrahydrofuran under the stirring condition, heating to 65-75 ℃, sequentially adding the intermediate obtained in the step S1 and p-toluenesulfonic acid, stirring for 4-9h, cooling to room temperature, performing solid-liquid separation, taking liquid, and performing reduced pressure distillation, washing and drying to obtain succinic acid derivatives, wherein the structural formula of the succinic acid derivatives is as follows: ; S3, dispersing gelatin in a phosphate buffer solution under the stirring condition, heating to 50-70 ℃, stirring for 0.5-1.5 h, adding the succinic acid derivative obtained in the step S2, continuously stirring for 2-4h, adjusting the pH value to 6-7, and dialyzing and freeze-drying to obtain modified gelatin, wherein the mass ratio of the gelatin to the phosphate buffer solution to the succinic acid derivative is 10:80-120:2-4; the molar ratio of the (2R, 3R) - (-) -epoxy succinic acid to the N-ethylmorpholine to the 4-methyl-1-amyl alcohol in the step S1 is 1:0.02-0.05:0.6-0.8, and the addition amount of the (2R, 3R) - (-) -epoxy succinic acid in the 2-methyltetrahydrofuran is 0.25-0.45g/mL; the molar ratio of the 3, 5-di-tert-butyl-4-hydroxybenzoic acid to the intermediate to the p-toluenesulfonic acid in the step S2 is 1:0.7-0.9:0.03-0.06, and the addition amount of the 3, 5-di-tert-butyl-4-hydroxybenzoic acid in the 2-methyltetrahydrofuran is 0.2-0.4g/mL.
  2. 2. The composite probiotic capsule of claim 1, wherein the emulsifier is one of sucrose fatty acid ester, polyglycerol fatty acid ester, tween-80, beeswax.
  3. 3. A composite probiotic capsule according to claim 1, characterized in that the concentration of the probiotic liquid is not less than 10 8 cfu/g.
  4. 4. The preparation method of the composite probiotic capsule according to any one of claims 1 to 3, which is characterized by comprising the steps of uniformly mixing probiotic bacteria liquid, fructo-oligosaccharide, tea polyphenol, grape polyphenol, mannooligosaccharide, isomaltulose and emulsifying agent to obtain a core material, uniformly mixing glycerol and distilled water, heating to 60-80 ℃, sequentially adding modified gelatin, hydroxypropyl starch and chitosan, stirring for 10-30min, sterilizing, cooling to room temperature to obtain a capsule wall material, mixing the core material and the capsule wall material, homogenizing under high pressure, and spray drying.
  5. 5. The method for preparing a composite probiotic capsule according to claim 4, wherein the homogenizing pressure of the high-pressure homogenizing treatment is 40-60MPa, and the homogenizing time is 10-20s.
  6. 6. The method for preparing the composite probiotic capsules according to claim 4, wherein the air inlet temperature of spray drying is 120-150 ℃, the air outlet temperature is 70-90 ℃, and the feeding flow rate is 10-20mL/min.

Description

Composite probiotic capsule and preparation method thereof Technical Field The invention belongs to the technical field of microcapsules, and particularly relates to a composite probiotic capsule and a preparation method thereof. Background Normally, more than 100 and billions of different bacteria inhabit in the intestinal tract of a human body, and are mutually restricted and coexist in common, wherein the bacteria are harmful to the human body, are called harmful bacteria, and the bacteria are beneficial to the human body, are called beneficial bacteria (namely probiotics). The probiotics have the main effects of promoting the health of intestinal digestive system, regulating immune function, relieving lactose intolerance symptoms, reducing serum cholesterol and the like, and are applied to a plurality of fields of medicines, foods, health care products and the like along with the rapid development of the probiotics industry. However, the existing probiotic products generally have the problems of low bacterial survival rate, unstable bacterial count, short product shelf life, unstable product quality and the like. The microcapsule encapsulation technology enables probiotics to be free from adverse environmental influences such as pH, high temperature, high humidity oxygen and the like by embedding the probiotics into a larger matrix, and effectively improves the activity of the probiotics and the stability of the product quality. The invention provides a preparation method of a probiotic-cod liver oil combined capsule, which comprises the steps of firstly mixing glycerol, gelatin and ultrapure water to prepare a glue solution, then mixing cod liver oil, vitamin E and probiotic freeze-dried powder to prepare a content feed liquid, finally pressing the content feed liquid and the glue solution into soft capsules, and shaping and drying to obtain a finished product, wherein the invention combines all the components, balances nutrition, simultaneously plays the original effects of all the components, can be mutually cooperated, and has good effects in the aspects of improving immunity, promoting brain development, preventing night blindness and the like; the invention provides a preparation method of a slimming probiotic soft capsule, which comprises the steps of firstly mixing solid materials with an oil solution to prepare a content feed liquid, then mixing glycerol, xylitol, purified water and gelatin to prepare a capsule soft shell feed liquid, finally pressing the content feed liquid and the capsule soft shell feed liquid into a soft capsule, and shaping and drying to obtain a finished product; the raw materials such as gelatin and glycerol are adopted as soft shell materials of the capsule in the above patent, the gelatin is derived from collagen and is a natural biological polymer material, and the gelatin has the advantages of biodegradability, good biocompatibility and gel property, low cost and the like, is a traditional pharmaceutical auxiliary material widely applied in the medicine field, is safe and effective when the gelatin is used as a capsule wall material of the capsule, but the defects of the gelatin are more and more obvious along with the expansion of the application requirement of the gelatin. Due to the existence of aldehyde groups, imino groups and other groups, the gelatin product is easy to crosslink in the storage process, the gelatin product is oxidized by air in the preparation and long-term storage processes, the crosslinking of the gelatin product is further promoted, a compact crosslinked reticular structure is generated after the crosslinking reaction of the gelatin capsule, the problems of difficult disintegration, reduced dissolution rate, reduced bioavailability and the like of the capsule can be caused, in addition, under the normal feeding condition, mixed foods usually enter the small intestine after staying in the stomach for 4-6 hours, and probiotics are easy to be killed due to the influence of gastric acid, so that the capsule material also has excellent acid resistance, thereby ensuring that the probiotics can effectively colonize in the small intestine, fully playing the beneficial role of the probiotics, and further improving the bioavailability of the capsule product. Disclosure of Invention Aiming at the defects of the prior art, the invention aims to provide a composite probiotic capsule, which has excellent storage stability, oxidation resistance, gastric juice erosion resistance and dissolution property under the condition of neutral alkali, namely has better enteric solubility, and a preparation method thereof. The technical scheme adopted by the invention for achieving the purpose is as follows: The composite probiotic capsule comprises, by weight, 20-40 parts of a probiotic bacterial liquid, 5-8 parts of fructo-oligosaccharide, 2-4 parts of tea polyphenol, 2-4 parts of grape polyphenol, 5-8 parts of mannooligosaccharide, 3-7 parts of isomaltitol and 1-3