CN-118048365-B - ZmSKI3 gene related to disease resistance of corn and application thereof

Abstract

The invention discloses ZmSKI gene related to corn disease resistance and application thereof, belonging to the field of biotechnology. The invention identifies ZmSKI genes in corn for the first time, and proves that ZmSKI genes control the resistance of corn to curvularia leaf spot, particularly, the expression of ZmSKI genes is inhibited, the resistance of corn to curvularia leaf spot can be enhanced, and the over-expression of ZmSKI genes can weaken the resistance of corn to curvularia leaf spot. The ZmSKI gene mutation obtained by the invention obviously activates the disease-resistant immune response of corn, is a new corn disease-resistant breeding gene resource, and provides an important molecular target for breeding resistant corn.

Inventors

• GOU MINGYUE
• LI JIANKUN
• GAO JIE
• MU XIAOHUAN
• YU YANWEN
• CHEN MENGYAO

Assignees

• 河南农业大学

Dates

Publication Date

20260512

Application Date

20240305

Claims (6)

1. 1. An application of ZmSKI gene related to corn disease resistance in regulating corn disease resistance, which is characterized in that the disease resistance of corn is enhanced by stopping translation of a corn ZmSKI3 protein encoded by ZmSKI gene in advance; the disease resistance of the corn is the resistance of the corn to curvularia leaf spot; The nucleotide sequence of ZmSKI gene is shown as SEQ ID NO. 1.
2. 2. The use according to claim 1, wherein the curvularia leaf spot comprises curvularia leaf spot caused by curvularia lunata (Curvularia lunata).
3. 3. A method for enhancing disease resistance of corn is characterized in that the resistance of corn to curvularia leaf spot can be enhanced by stopping translation of a ZmSKI gene-encoded corn ZmSKI3 protein in advance, wherein the nucleotide sequence of a ZmSKI gene is shown as SEQ ID NO. 1; the disease resistance of the corn is the resistance of the corn to curvularia leaf spot.
4. 4. The application of ZmSKI gene related to corn disease resistance in breeding corn disease resistant variety can improve corn resistance to curvularia leaf spot by stopping translation of corn ZmSKI3 protein coded by ZmSKI gene in advance, and the nucleotide sequence of ZmSKI gene is shown as SEQ ID NO. 1.
5. 5. A method for cultivating a maize disease-resistant variety is characterized by comprising the step of stopping translation of a ZmSKI gene-encoded maize ZmSKI protein in advance to enhance disease resistance of an obtained maize plant, wherein the nucleotide sequence of a ZmSKI gene is shown as SEQ ID NO.1, and the disease resistance is resistance to Curvularia leaf spot.
6. 6. The method of claim 5, wherein the curvularia leaf spot comprises curvularia leaf spot caused by curvularia lunata.

Description

ZmSKI3 gene related to disease resistance of corn and application thereof Technical Field The invention relates to the technical field of biology, in particular to ZmSKI gene related to corn disease resistance and application thereof. Background Corn is one of three grain crops in China, but the diseases frequently occurring in recent years have great influence on the yield and quality of the corn, so that the cultivation of the disease-resistant variety of the corn is urgent. However, due to the lack of genetic resources for resistance used in maize breeding, the genetic basis is narrow, which is very detrimental to the breeding and molecular improvement of disease resistant varieties, and by obtaining and introducing disease resistant genes into the targeted maize line or variety has proven to be an effective approach. However, the lack of the disease-resistant gene of the corn makes the progress of disease-resistant breeding of the corn slow. Although some reports of corn resistance gene cloning and application exist at present, the demands of corn disease-resistant breeding resistance genes are far from being met. Therefore, the discovery of genes with resistance to different diseases is a problem to be solved in the current maize disease-resistant breeding. At present, the corn disease-resistant breeding lacks disease-resistant genes, particularly the curvularia leaf spot disease-resistant genes are particularly lacking, and the breeding development of corn disease-resistant molecules is hindered. Therefore, the identification of genes for regulating growth and disease resistance in corn has great significance for genetic improvement of corn disease resistance. Previous studies in humans, yeast, arabidopsis, and rice have shown that cofactors SKIs of the mRNA splice complex may play a very important role in growth and disease resistance. However, the genetic information of the SKIs cofactor of maize and its role in disease resistance and growth in maize is currently unknown. Disclosure of Invention The invention aims to provide ZmSKI gene related to corn disease resistance and application thereof, so as to solve the problems in the prior art, the invention firstly identifies ZmSKI gene of corn, verifies the function of negative regulation of corn curvularia leaf spot resistance of ZmSKI gene by using EMS mutant and transgenic technology, and provides new gene resources for corn disease resistance breeding. In order to achieve the above object, the present invention provides the following solutions: the invention provides ZmSKI genes related to corn disease resistance, and the nucleotide sequence of the ZmSKI genes is shown as SEQ ID NO. 1. The invention also provides application of ZmSKI gene in regulating and controlling corn disease resistance, inhibiting expression of ZmSKI gene to enhance corn disease resistance, and over-expressing ZmSKI gene to weaken corn disease resistance, wherein the corn disease resistance is corn resistance to curvularia leaf spot. Further, the curvularia leaf spot includes curvularia leaf spot caused by curvularia lunata (Curvularia lunata). The invention also provides a method for enhancing the disease resistance of corn, which comprises the step of inhibiting the expression of a ZmSKI gene of corn, wherein the nucleotide sequence of the ZmSKI gene is shown as SEQ ID NO. 1; the disease resistance of the corn is the resistance of the corn to curvularia leaf spot. Further, inhibiting expression of the ZmSKI gene includes prematurely stopping translation of the maize ZmSKI3 protein. The invention also provides application of ZmSKI gene in cultivating maize disease-resistant variety. The invention also provides a method for cultivating the maize disease-resistant variety, which comprises the step of enhancing the disease resistance of the obtained maize plant by inhibiting the expression of a maize ZmSKI gene, wherein the nucleotide sequence of the ZmSKI gene is shown as SEQ ID NO. 1. Further, the disease resistance is resistance to curvularia leaf spot. Further, the curvularia leaf spot includes curvularia leaf spot caused by curvularia lunata. Further, inhibiting expression of the ZmSKI gene includes prematurely stopping translation of the maize ZmSKI3 protein. The invention discloses the following technical effects: The ZmSKI gene is identified in corn for the first time, and the gene is proved to be capable of regulating and controlling the resistance of the corn to curvularia leaf spot. In the specific embodiment of the invention, two mutants Zmski-1 and Zmski3-2 with the translation of ZmSKI protein terminated in advance are obtained by screening EMS mutant library, and the plants Zmski-1 and Zmski-2 are found to be short in development but the resistance to corn curvularia leaf spot is obviously improved through phenotypic observation. Meanwhile, a ZmSKI over-expressed transgenic line is further obtained, and the growth state of the transgenic line is not affected, but