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CN-118080006-B - Catalyst for degrading polysaccharide and preparation method and application thereof

CN118080006BCN 118080006 BCN118080006 BCN 118080006BCN-118080006-B

Abstract

A catalyst for degrading polysaccharide and a preparation method and application thereof belong to the technical field of polysaccharide degradation. The invention provides a preparation method of a catalyst for degrading polysaccharide, which comprises the steps of weighing fumaric acid and hydrogen peroxide, mixing and preparing the catalyst. The molar ratio of fumaric acid to hydrogen peroxide is 0.5-2:0.5-2. The invention also provides a catalyst for degrading polysaccharide, which is obtained by the preparation method. The catalyst prepared from fumaric acid and hydrogen peroxide is used for degrading the galactomannan, so that the polysaccharide degradation efficiency can be improved, the yield of the galactomannan and galactomannan oligosaccharide with small molecular weight is high, and the benefit is improved.

Inventors

  • WANG LIQUN
  • LIN HONGYAN
  • Ruan Lingyu
  • ZHANG YUE
  • ZHAO YUQI
  • CHENG CHEN
  • WU QIONG
  • XU XINYE
  • LIN XINYI
  • WANG DANDAN
  • TAO YUHENG
  • QING QING

Assignees

  • 常州大学

Dates

Publication Date
20260505
Application Date
20240117

Claims (10)

  1. 1. A method for preparing a catalyst for degrading polysaccharides, comprising the steps of: and (3) weighing fumaric acid and hydrogen peroxide, mixing, and preparing the catalyst.
  2. 2. The process according to claim 1, wherein the molar ratio of fumaric acid to hydrogen peroxide is 0.5-2:0.5-2.
  3. 3. A catalyst for degrading polysaccharides, characterized by being obtained by the preparation method according to claim 1.
  4. 4. Use of the catalyst according to claim 3 for degrading polysaccharides.
  5. 5. The method of claim 4, wherein the degradation is carried out by weighing polysaccharide, adding catalyst, and degrading to obtain degraded polysaccharide solution; The degradation condition is that the temperature is 70-120 ℃ and the time is 30-120 min.
  6. 6. The use according to claim 4, wherein the polysaccharide is a galactomannan.
  7. 7. The use according to claim 6, wherein the galactomannans are degraded to obtain high molecular weight galactomannans, small molecular weight galactomannans and galactomannooligosaccharides.
  8. 8. The use according to claim 7, wherein the specific process of galactomannan degradation is: (1) Weighing galactomannan, adding a catalyst, and degrading to obtain polysaccharide degradation liquid; (2) Centrifuging the polysaccharide degradation solution to obtain a first supernatant, and adding an organic solvent to obtain a second supernatant and a precipitate, namely high molecular weight galactomannan; (3) Adding an organic solvent into the second supernatant obtained in the step (2) to obtain a third supernatant and a precipitate, namely the small molecular weight galactomannan; (4) Concentrating and drying the third supernatant obtained in the step (3) to obtain the galactomannan oligosaccharide.
  9. 9. The use according to claim 8, wherein the organic solvent is selected from one of ethanol, acetone or butanol; the volume content of the organic solvent in the first supernatant liquid in the step (2) is 40%; The volume content of the organic solvent in the second supernatant liquid in the step (3) is 65%; The temperature of concentration in the step (4) is 50-100 ℃.
  10. 10. The method for preparing the galactomannan is characterized in that the seed containing the galactomannan is taken and crushed, hot water is used for extraction to obtain an extracting solution, an organic solvent is added into the extracting solution to obtain a precipitate A, the precipitate A is redissolved after drying, protease is added and the supernatant is obtained after centrifugation, the organic solvent is added to obtain a precipitate B, and the precipitate B is washed and dried to obtain a galactomannan solid; The seed containing galactomannan is Acacia seeds; The organic solvent is selected from one of ethanol, acetone or butanol; The addition amount of the protease is 5-20U/g.

Description

Catalyst for degrading polysaccharide and preparation method and application thereof Technical Field The invention belongs to the technical field of polysaccharide degradation, and particularly relates to a catalyst for degrading polysaccharide, a preparation method and application thereof. Background At present, small molecule galactomannans and galactomannooligosaccharides are getting more and more attention because of their excellent immunity enhancing effect, and the main production processes include an enzymolysis method, an acidolysis method and an oxidation method. In the enzymolysis process, galactose branches attack the mannose main chain by beta-mannase to form steric hindrance, and alpha-galactosidase needs to be added for auxiliary degradation. In order to increase the galacto-oligosaccharide yield, it is necessary to reduce the activity of β -mannosidase in the enzyme solution as much as possible. In addition, the enzyme hydrolysis time is longer, so that the efficiency of the production process is reduced, and the production cost is increased. In recent years, the technology for preparing the oligosaccharide by catalyzing the organic acid has been widely researched due to the relatively safe reaction conditions and green process, but the organic acid degradation method has the defect of higher reaction temperature (100 ℃), and is not beneficial to industrial production. Hydrogen peroxide is used more in the oxidation process, but its degradation efficiency is lower. These methods have advantages and disadvantages and all suffer from cost problems, including time costs, and also subsequent separation costs. Therefore, development of a low-cost and efficient oligosaccharide preparation method is urgently required. With the continued development of technology, some advanced oxidation techniques, such as the Fenton reaction, are applied to degrade polysaccharides and are continually improved and optimized. Fenton reaction refers to a reaction of degrading organic matters by using a mixed solution with strong oxidizing property composed of hydrogen peroxide and ferrous ions. However, this method has a problem of metal ion residue, thereby increasing the cost of subsequent separation. Disclosure of Invention In view of the above problems in the prior art, an object of the present invention is to design and provide a catalyst for degrading polysaccharides, and a preparation method and application thereof. The catalyst prepared by fumaric acid and hydrogen peroxide is used for degrading polysaccharide, so that the polysaccharide degradation efficiency is greatly improved. In order to achieve the above purpose, the present invention adopts the following technical scheme: in one aspect, the invention provides a method for preparing a catalyst for degrading polysaccharide, which is characterized by comprising the following steps: and (3) weighing fumaric acid and hydrogen peroxide, mixing, and preparing the catalyst. In the preparation method, the mol ratio of fumaric acid to hydrogen peroxide is 0.5-2:0.5-2. In a second aspect, the present invention provides a catalyst for degrading polysaccharides, obtainable by the preparation process. The catalyst is applied to degrading polysaccharide. The method for degrading the polysaccharide comprises the steps of weighing polysaccharide, adding a catalyst, and degrading to prepare a degraded polysaccharide solution; preferably, the degradation condition is that the temperature is 70-120 ℃ and the time is 30-120min. For said use, the polysaccharide is galactomannan. The application of the method is that the galactomannan is degraded to obtain high molecular weight galactomannan, small molecular weight galactomannan and galactomannan oligosaccharide. The specific process of the degradation of the galactomannan is as follows: (1) Weighing galactomannan, adding a catalyst, and degrading to obtain polysaccharide degradation liquid; (2) Centrifuging the polysaccharide degradation solution to obtain a first supernatant, and adding an organic solvent to obtain a second supernatant and a precipitate, namely high molecular weight galactomannan; (3) Adding an organic solvent into the second supernatant obtained in the step (2) to obtain a third supernatant and a precipitate, namely the small molecular weight galactomannan; (4) Concentrating and drying the third supernatant obtained in the step (3) to obtain the galactomannan oligosaccharide. The application is that the organic solvent is selected from one of ethanol, acetone or butanol; the content of the organic solvent in the first supernatant liquid in the step (2) is 40 percent; the content of the organic solvent in the second supernatant liquid in the step (3) is 65 percent; The temperature of concentration in the step (4) is 50-100 ℃. The preparation method of the galactomannan comprises the steps of taking the seeds of the galactomannan, crushing, extracting with hot water to obtain an extracting solution, adding a