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CN-118160811-B - Method for producing protein feed by microbial two-stage solid state fermentation and guniting of corn husks

CN118160811BCN 118160811 BCN118160811 BCN 118160811BCN-118160811-B

Abstract

The invention discloses a method for producing protein feed by two-stage solid state fermentation of guniting corn husks by microorganisms, wherein the protein feed is prepared by two-stage solid state fermentation of guniting corn husks and urea by compound microorganisms, the compound microorganisms comprise aspergillus niger, saccharomyces cerevisiae, bacillus licheniformis and pediococcus pentosaceus, the two-stage solid state fermentation comprises two stages of aerobic solid state fermentation and anaerobic solid state fermentation, the crude protein content of the obtained guniting corn skin protein feed product is 29% -36%, the nitrogen-containing folded protein content of true protein, polypeptide and amino acid is 23% -29%, the nitrogen-containing folded protein content of urea and inorganic ammonia nitrogen is only 3% -6%, and the feed fermentation process can effectively convert low-cost urea into high-value protein, amino acid and polypeptide which can be used by animals, so that the protein production cost is reduced.

Inventors

  • WANG ZHI
  • MI YIFAN
  • HE DAI
  • WANG FEI
  • GUO XIAOMIN
  • Cai Yafan
  • WANG SHILEI
  • LIU DONG
  • YING HANJIE

Assignees

  • 郑州大学

Dates

Publication Date
20260505
Application Date
20240425

Claims (6)

  1. 1. The method for producing the protein feed by microbial two-stage solid state fermentation and guniting of corn husks is characterized by comprising the following steps: Inoculating an aerobic bacterial liquid to the fermentation raw material to perform aerobic solid-state fermentation to obtain an aerobic fermentation product; the aerobic bacteria liquid comprises aspergillus niger liquid, saccharomyces cerevisiae liquid and bacillus licheniformis liquid; the anaerobic bacteria liquid comprises Pediococcus pentosaceus bacteria liquid; the fermentation raw materials comprise dry matters, wherein the dry matters comprise gunite corn husks and urea, and the mass ratio of the urea in the dry matters is 1% -5%; the aerobic solid fermentation is carried out at the temperature of 22-44 ℃ for 72-168 hours; the anaerobic solid state fermentation is carried out at the temperature of 20-40 ℃ for 24-72 hours; The number of viable bacteria of Aspergillus niger in the Aspergillus niger liquid is 0.5-2×10 7 CFU/g, the number of viable bacteria of Saccharomyces cerevisiae in the Saccharomyces cerevisiae liquid is 4-6×10 6 CFU/g, the number of viable bacteria of Bacillus licheniformis in the Bacillus licheniformis liquid is 0.1-1.0×10 6 CFU/g, and the number of viable bacteria of Pediococcus pentosaceus in the Pediococcus pentosaceus liquid is 0.1-1.0×10 6 CFU/g; the volume ratio of the aspergillus niger liquid, the saccharomyces cerevisiae liquid, the bacillus licheniformis liquid and the pediococcus pentosaceus liquid is 1-2: 1-2, 0.5-1 and 0.5-1.
  2. 2. The method of claim 1, wherein the aspergillus niger is aspergillus niger (Aspergillus niger) ATCC 16888, the saccharomyces cerevisiae is saccharomyces cerevisiae (Saccharomyces cerevisiae) ATCC 18824, the bacillus licheniformis is bacillus licheniformis (Bacillus licheniformis) ATCC 14580, and the pediococcus pentosaceus is pediococcus pentosaceus (Pediococcus pentosaceus) ATCC 33316.
  3. 3. The method according to claim 1, wherein the dry matter accounts for 10% -80% of the total mass of the fermentation.
  4. 4. The method according to claim 3, wherein the mass ratio of the aerobic bacteria liquid to the dry matter is 1% -50%.
  5. 5. The protein feed prepared by the method according to any one of claims 1 to 4, wherein the protein feed contains 29% -36% of crude protein, 3% -6% of urea and inorganic ammonia nitrogen folded protein, 8% -10% of crude fiber, 4.2% -4.8 of pH value, 6% -9×10 6 CFU/g of saccharomycetes viable count, 3% -7×10 6 CFU/g of bacillus licheniformis viable count, 5% -6×10 6 CFU/g of lactic acid bacteria viable count, 61.2 mug/kg of zearalenone and 12.2 mug/kg of aflatoxin.
  6. 6. Use of the protein feed of claim 5 in the preparation of an animal feed.

Description

Method for producing protein feed by microbial two-stage solid state fermentation and guniting of corn husks Technical Field The invention relates to the field of biological feeds, in particular to a method for producing protein feeds by microbial two-stage solid state fermentation and guniting of corn husks. Background In recent years, along with the continuous expansion of the scale of the breeding industry in China, the problem of serious shortage of feed protein resources is faced. The waste residue, waste and waste liquid of the agricultural and sideline product processing industry are treated by the biotechnology to produce the biological feed or additive, so that the pollution of the waste biomass to the environment can be reduced, the high-value utilization of waste resources can be realized, the soybean meal, corn and the like can be replaced in the breeding industry, the current situation of feed protein shortage is relieved, and the green sustainable development of the breeding industry in China is promoted. The sprayed corn husks are byproducts of corn wet processing, are prepared by spraying corn steep liquor on corn husks and drying, and are currently about 42.9 ten thousand tons in annual output in China. The gunite corn husks with the crude protein content of more than 15 percent and the crude fiber content of less than 15 percent can be directly fed as feed, and the market price is about 1500 yuan/ton. However, the spraying corn husks have unbalanced nutrition structures, which can lead to the problems of complex feeding process, low digestibility and the like. In addition, the gunite corn flakes are easy to generate high mycotoxin and poor palatability in the storage process, and the application of the gunite corn flakes in the feed industry is directly inhibited. Although the prior China patent with the application number 2018115725454 ferments the gunite corn husks, the number of probiotics and the amino acid structure of the feed product are only improved, but the true protein content is reduced from 15% to 10.5%, which is reduced by 30% compared with the prior fermentation, and the loss of protein is caused. The low-cost non-protein nitrogen is converted by using the feeding microorganism through the biological fermentation technology, so that the content and the value of the sprayed corn skin protein can be effectively improved. However, at present, the utilization rate of microorganisms (such as Saccharomyces cerevisiae, bacillus, lactobacillus and the like) for non-protein nitrogen urea is generally only 30%, so that the selection of microorganisms or microorganism combinations with the capability of assimilating cheap non-protein nitrogen (such as urea) has great significance for efficient production of protein feed. In addition, the biological fermentation can effectively reduce the content of crude fiber in the gunite corn husks, improve the palatability and the content of probiotics, reduce the pH value, avoid mould pollution and prolong the preservation time, and has positive significance for improving the usage amount and the use effect of the gunite corn husks in the feed, the economic value of the gunite corn husks and solving the feed gap. Disclosure of Invention The invention aims to solve the technical problem of providing a method for producing protein feed by microbial two-stage solid state fermentation and guniting of corn husks. In order to solve the technical problems, the invention discloses a method for producing protein feed by two-stage solid state fermentation and slurry spraying of corn husks by utilizing microorganisms with urea assimilation capability. The specific technical scheme is as follows: A method for producing protein feed by microbial two-stage solid state fermentation of guniting corn husks comprises inoculating aerobic bacteria liquid to fermentation raw materials for aerobic solid state fermentation to obtain an aerobic fermentation product, and inoculating anaerobic bacteria liquid to the aerobic fermentation product for anaerobic solid state fermentation to obtain protein feed; The aerobic bacteria liquid comprises aspergillus niger liquid, saccharomyces cerevisiae liquid and bacillus licheniformis liquid, and the aspergillus niger and the saccharomyces cerevisiae are microorganisms with urea assimilation capability. The anaerobic bacteria liquid comprises Pediococcus pentosaceus bacteria liquid. The Aspergillus niger is Aspergillus niger NIGER ATCC 16888, the Saccharomyces cerevisiae is Saccharomyces cerevisiae CEREVISIAE ATCC 18824, the Bacillus licheniformis is Bacillus licheniformis Bacillus licheniformis ATCC 14580, and the Pediococcus pentosaceus is Pediococcus pentosaceus Pediococcus pentosaceus ATCC 33316. The number of viable bacteria of Aspergillus niger in the Aspergillus niger liquid is 0.5-2X 10 7 CFU/g, the number of viable bacteria of Saccharomyces cerevisiae in the Saccharomyces cerevisiae liquid is 4-6X 10 6 CFU/g, the number o