CN-118236441-B - Polygonatum kingianum extract and preparation method and application thereof

Abstract

The invention belongs to the technical field of traditional Chinese medicine extraction, and particularly relates to a Polygonatum kingianum extract and a preparation method and application thereof. According to the invention, dry Polygonatum sibiricum powder is taken as a raw material, ethanol solution is used for extraction, a sugar-containing part can be effectively removed through gradient elution of a polystyrene type macroporous resin column, the purpose of enriching micromolecular substances such as flavone, saponin and amide is achieved, the obtained first extract has a good recovery effect on reduction of liver cell activity of amyloidosis caused by lysozyme and reduction of liver cell activity caused by alcohol, then the first extract is subjected to normal phase silica gel column chromatography elution and layering, and the second extract containing high isoflavone compounds has a remarkable recovery effect on reduction of liver cell activity caused by lysozyme and reduction of liver cell activity caused by alcohol, so that the Polygonatum sibiricum can be applied to preparation of drugs for reversing hepatic amyloidosis and alcoholic liver injury, and has important clinical application value.

Inventors

• ZHANG YU
• HUANG YUN
• WEN HONGYAN
• WANG HUI
• GUO LINGLI

Assignees

• 中国科学院昆明植物研究所
• 河北医科大学

Dates

Publication Date

20260508

Application Date

20240401

Claims (3)

1. 1. A preparation method of a Polygonatum kingianum extract for preventing and/or treating alcoholic liver injury is characterized by comprising the following steps of: Mixing Polygonatum kingianum powder and ethanol solution for leaching, concentrating the obtained leaching solution to obtain extract, wherein the dosage ratio of the Polygonatum kingianum powder to the ethanol solution is 1kg:6L, the volume fraction of ethanol in the ethanol solution is 80%, the leaching temperature is 10-30 ℃, the leaching times are 4, and the leaching time is 3 days; Mixing the extract with water, loading on a D101 polystyrene macroporous resin column by adopting a wet method, eluting by sequentially using water, 20% volume fraction ethanol solution and 90% volume fraction ethanol solution as an eluent, collecting an eluent obtained by eluting with the 90% volume fraction ethanol solution, and concentrating under reduced pressure to obtain an extract Fr.C; Separating the extract Fr.C by a silica gel column, sequentially carrying out gradient elution by using eluent with the volume ratio of petroleum ether to acetone of 100:1, 50:1 and 20:1, collecting the components obtained by eluting by using the eluent with the volume ratio of petroleum ether to acetone of 20:1, concentrating until the volume fraction of ethanol is not more than 5%, and drying to obtain the Polygonatum kingianum extract.
2. 2. The Polygonatum kingianum extract obtained by the preparation method of claim 1, wherein the content of high isoflavone in the Polygonatum kingianum extract is 2-4wt%.
3. 3. Use of the extract of Polygonatum kingianum of claim 2 for the preparation of a medicament for the prevention and/or treatment of alcoholic liver injury.

Description

Polygonatum kingianum extract and preparation method and application thereof Technical Field The invention belongs to the technical field of traditional Chinese medicine extraction, and particularly relates to a Polygonatum kingianum extract and a preparation method and application thereof. Background Liver amyloidosis is a part of systemic amyloid lesions, and is called liver amyloidosis when amyloid material is deposited in the liver resulting in pathological changes in the liver and related clinical manifestations. Liver amyloidosis is a clinically less common disease, can be liver manifestation of systemic amyloidosis, and can also be secondary to chronic infection, tuberculosis, rheumatoid arthritis and other diseases. Common clinical manifestations are hepatomegaly, ascites, massive proteinuria and hypoproteinemia, which are easily misdiagnosed as fatty liver, bai Cha syndrome, etc., and even cause long-term misdiagnosis. At present, no effective specific therapy exists for the disease, but early diagnosis and early intervention can treat related complications in time, and the disease has a certain effect of improving prognosis of patients. Liver amyloidosis is mainly treated by medicines, generally cannot be cured, and related symptoms can be improved through positive treatment. Alcoholic liver injury is a common liver disease caused by long-term and large-scale alcohol consumption, and is usually manifested as alcoholic fatty liver in early stage, if the alcoholic liver injury can not be effectively treated, alcoholic hepatitis, liver fibrosis, liver cirrhosis, hepatocellular carcinoma and the like can be possibly developed, due to the complexity of pathogenesis, no specific preventive and therapeutic drug exists in clinic at present, and the therapeutic approach is mainly alcohol withdrawal, nutrition therapy, drug therapy, combination therapy and the like. Currently, liver protection medicines sold in the market are mainly western medicines, and toxic and side effects are very easy to generate in the long-term medicine taking process. Polygonatum kingianum (Polygonatum kingianum Coll. Et Hemsl.) is a perennial herb of Polygonatum (Polygonatum) of Asparaginaceae (ASPARAGACEAE), also known as festival height, ma Weigen, ipomoea batatas, and Opuntia Dillenii. Polygonatum kingianum contains active compounds beneficial to the body, such as Polygonatum kingianum polysaccharide, alkaloid, saponin, flavone, sterol amino acid and the like, has the effects of nourishing yin, strengthening spleen, moistening lung, tonifying kidney and the like, is used for improving immunity, resisting oxidization, improving cardiovascular health, treating qi deficiency, lung deficiency, dry cough, essence and blood deficiency and the like, is also an important component of dozens of nourishing traditional Chinese medicine compounds or Chinese patent medicines, and is valued by folks and medical pharmaceutical families from ancient times. Yuan Huang Jing is also one of three species of Polygonatum sibiricum base plants specified in Chinese pharmacopoeia (2020 edition), also known as "Yuan Tou Huang Jing". At present, polygonatum kingianum extract applied to reversing hepatic amyloidosis and alcoholic liver injury is not reported. Disclosure of Invention Accordingly, the present invention is directed to a Polygonatum kingianum extract, and a preparation method and application thereof. The Polygonatum kingianum extract has recovery effect on reducing the activity of liver cells of amyloidosis caused by lysozyme and reducing the activity of liver cells caused by alcohol. In order to achieve the above object, the present invention provides the following technical solutions: The invention provides a preparation method of a Polygonatum kingianum extract, which comprises a first Polygonatum kingianum extract and/or a second Polygonatum kingianum extract, wherein the first Polygonatum kingianum extract contains flavone, amide and saponin, and the second Polygonatum kingianum extract contains high isoflavone, and the preparation method of the first Polygonatum kingianum extract is characterized by comprising the following steps of: Mixing rhizoma Polygonati powder with ethanol solution, leaching, and concentrating the obtained leaching solution to obtain extract; mixing the extract with water, and removing impurities by using a polystyrene macroporous resin column to obtain a first Polygonatum kingianum extract; the preparation method of the second Polygonatum kingianum extract comprises the following steps: Separating the first Polygonatum kingianum extract by a silica gel column, sequentially performing gradient elution by using an eluent with a first volume ratio, a second volume ratio and a third volume ratio of petroleum ether and acetone, and collecting components obtained by eluting by the eluent with the third volume ratio of petroleum ether and acetone to obtain the second Polygonatum kingianum extract, wherein the first vol