CN-118239609-B - Method for relieving carbon dioxide to inhibit denitrification and promoting denitrification

Abstract

The invention discloses a method for relieving the inhibition of carbon dioxide on denitrification and promoting the denitrification. The method comprises the steps of inputting 0.3% -20% of CO 2 into a closed reactor, inoculating acetic acid-producing proteobacteria Proteiniphilum into a denitrification system of denitrifying microorganisms P.denificans, and achieving the effects of relieving inhibition of CO 2 on denitrification and promoting denitrification efficiency simultaneously through interaction of the two bacteria, wherein the inoculation ratio of the P.denificans to Proteiniphilum is 1:2-1:15, the carbon nitrogen ratio of the denitrification system is 1-5, the pH value of the denitrification system is 6.0-9.0, and the denitrification culture temperature is 30-37 ℃. The invention promotes anaerobic denitrification by utilizing the interaction of Proteiniphilum and denitrifying microorganism P.Denitrificans, has low cost and no secondary pollution, can obviously relieve the inhibition effect of CO 2 , improves the nitrate removal effect and reduces the accumulation of nitrite and nitrous oxide.

Inventors

• CHEN YINGUANG
• Wu Meirou
• ZHANG XUEMENG
• QIN ZHIYI
• Peng Haojin
• FAN XINYUN
• LIU CHAO
• LIU QIMENG
• WANG SHUAI

Assignees

• 同济大学

Dates

Publication Date

20260508

Application Date

20240418

Claims (4)

1. 1. A method for relieving the inhibition of denitrification by carbon dioxide and promoting denitrification, which is characterized in that paracoccus denitrificans and proteobacteria acetoacidophilus are added to synergistically relieve the inhibition of denitrification by carbon dioxide and promote microbial denitrification; The method comprises the following steps: s1, adding a culture medium and trace elements into a reactor, performing anaerobic treatment, then filling carbon dioxide and sterilizing to obtain an anaerobic denitrification culture medium system; S2, providing a paracoccus denitrificans suspension and a proteophilus acetate production suspension; S3, inoculating the paracoccus denitrificans suspension and the acidophilic acetoacidophilus suspension into the anaerobic denitrification culture medium system obtained in the step S1, and performing anaerobic denitrification culture; In the step S1, the mole percentage of the carbon dioxide is 0.3% -20%, and the carbon-nitrogen ratio in the culture medium is 1% -5; in the step S3, the carbon-nitrogen ratio of the anaerobic denitrification culture medium system is 1-5; The inoculation ratio of the paracoccus denitrificans suspension to the acidophilus acetogenic suspension is 1:2-1:15; the pH value of the anaerobic denitrification culture medium system is 6.0-9.0; The temperature of the anaerobic denitrification culture is 30-37 ℃.
2. 2. The method for alleviating carbon dioxide inhibition denitrification and promoting denitrification according to claim 1, wherein in step S1, the medium comprises a carbon source and an inorganic salt.
3. 3. The method for alleviating carbon dioxide inhibition denitrification and promoting denitrification according to claim 1, wherein in step S2, the initial OD 600 of the paracoccus denitrificans suspension is 0.01-0.02.
4. 4. The method for alleviating carbon dioxide inhibition denitrification and promoting denitrification according to claim 1, wherein in step S2, the initial OD600 of the acidophilic acetate suspension is 0.02-0.2.

Description

Method for relieving carbon dioxide to inhibit denitrification and promoting denitrification Technical Field The invention relates to the technical field of biological denitrification treatment, in particular to a method for relieving carbon dioxide inhibition denitrification and promoting denitrification, and especially relates to a method for relieving CO 2 inhibition of denitrification and promoting anaerobic denitrification efficiency by Proteiniphilum. Background With the development of industry and economy, the combustion of fossil fuels and the destruction of forests are greatly increased, the concentration of CO 2 in the atmosphere is gradually increased, and the increasing speed is also gradually increased. The elevated concentration of CO 2 in the atmosphere will cause a series of significant environmental problems such as ocean acidification and global warming. Carbon dioxide sequestration is considered to be an effective means of dealing with elevated concentrations of CO 2, but due to CO 2 leakage problems at the sequestration site, CO 2 concentrations around underground sequestration sites may rise dramatically, up to more than 100 times the atmospheric concentration, creating serious challenges for all types of living beings and environmental circulatory systems on earth. On the other hand, the use of a large amount of nitrogen-containing substances such as fertilizer causes serious pollution of nitrogen sources in the environment, and causes a series of environmental problems such as eutrophication of water bodies and pollution of groundwater. The biological denitrification is utilized to remove nitrate pollution in water, and people increasingly pay attention to the removal of nitrate pollution. However, CO 2 can have an inhibitory effect on the denitrification process, resulting in a slow down of the denitrification process, and there can also be some accumulation of toxic and deleterious intermediates of the nitrate reduction process (e.g., nitrite and nitrous oxide). Therefore, it is necessary to find a method for alleviating the inhibition of denitrification by CO 2, promoting the high speed of denitrification process without intermediate accumulation. Disclosure of Invention In order to solve the technical problems, the invention aims to provide a method for relieving the inhibition effect of carbon dioxide and simultaneously promoting the denitrification rate, which is characterized in that the inhibition effect of CO 2 on denitrification is relieved by adding cultured paracoccus denitrificans and acetoacidophilus in a reactor filled with CO 2 according to a certain proportion, the denitrification rate is improved, and the accumulation and release of a denitrification intermediate product N 2 O are reduced. In the present invention, a certain proportion of the mixed bacteria can alleviate the adverse effect of CO 2 on denitrification because of the synergistic effect between paracoccus denitrificans and acetoacidophil, and the adverse effect of CO 2 is resisted by producing a microbial secondary metabolite or enhancing the content of extracellular polymer in the system so as to recover and promote the denitrification process. The method is simply implemented by microorganism regulation and control, and has no secondary pollution. The invention aims to provide a method for relieving carbon dioxide to inhibit denitrification and promoting denitrification, which is used for synergistically relieving the inhibition of carbon dioxide on denitrification and promoting microbial denitrification by adding paracoccus denitrificans and acetoacidophil. In one embodiment of the invention, the method comprises the steps of: s1, adding a culture medium and trace elements into a reactor, performing anaerobic treatment, then filling carbon dioxide and sterilizing to obtain an anaerobic denitrification culture medium system; S2, providing a paracoccus denitrificans suspension and a proteophilus acetate production suspension; S3, inoculating the paracoccus denitrificans suspension and the acidophilic acetoacidophilus suspension into the anaerobic denitrification culture medium system obtained in the step S1, and performing anaerobic denitrification culture. In one embodiment of the present invention, in step S1, the mole percentage of carbon dioxide is 0.3% -20%. In one embodiment of the present invention, in step S1, the carbon-nitrogen ratio in the medium is 1 to 5. In one embodiment of the present invention, in step S1, the medium includes a carbon source and an inorganic salt. Further, the carbon source is glucose or sodium acetate. Further, the microelements comprise 7.30g/L disodium ethylenediamine tetraacetate, 2.50g/L ferrous sulfate heptahydrate, 0.02g/L manganese chloride, 0.242g/L sodium molybdate, 0.02g/L calcium chloride, 0.135g/L copper chloride crystal and 0.34g/L zinc chloride. Further, the inorganic salt comprises 2.16g/L potassium nitrate, 0.5g/L ammonium chloride, 0.07-0.1 g/L magnesi