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CN-118286129-B - Duying extract of Yunnan Tibetan and preparation method and application thereof

CN118286129BCN 118286129 BCN118286129 BCN 118286129BCN-118286129-B

Abstract

The invention provides a herba Duchesneae Indicae extract, and a preparation method and application thereof, wherein the preparation method comprises the following steps of (1) crushing herba Duchesneae Indicae, mixing with enzyme, then adopting a solvent to carry out negative pressure cavitation extraction to obtain a herba Duchesneae Indicae Du Yingcu extract, and (2) adopting macroporous resin to adsorb and elute the herba Duchesneae Indicae Du Yingcu extract obtained in the step (1) to obtain the herba Duchesneae Indicae extract. The method provided by the invention can effectively extract active substances in the Duying of the Yunnan Tibetan, and the obtained Duying extract has the advantages of high content of geraniin, high content of total flavone, good oxidation resistance, high hyaluronidase inhibition activity, good anti-inflammatory effect, high collagenase activity inhibition rate, economic and environment-friendly preparation method, and the used reagent can be recycled and reused and can be used as the active substances in health care products and cosmetics.

Inventors

  • YUAN YONGLEI
  • ZHOU ZIHAO
  • QU LIPING
  • WANG FEIFEI
  • MA XIAO
  • GUO ZHENYU

Assignees

  • 云南贝泰妮生物科技集团股份有限公司
  • 云南云科特色植物提取实验室有限公司
  • 美达丝(上海)生物科技有限公司

Dates

Publication Date
20260505
Application Date
20240402

Claims (9)

  1. 1. A method for preparing a dulcamara extract, comprising the steps of: (1) Pulverizing herba Duchesneae Indicae, mixing with enzyme, and extracting with solvent under negative pressure to obtain extract of herba Duchesneae Indicae Du Yingcu; (2) Adsorbing and eluting the extract of the Yunnan Tibetan Du Yingcu obtained in the step (1) by using macroporous resin to obtain the extract of the Yunnan Tibetan Duying; The enzyme in the step (1) is pectase, and the mass ratio of the enzyme to the Duying in Yunnan Tibetan is (1-3) 10000; the feed liquid ratio of the Yunnan Tibetan dulcin to the solvent in the step (1) is 1 (8-12) g/mL; the solvent is a combination of ethanol and water, wherein the concentration of the ethanol is 55% or 50%; the specific flow of the elution in the step (2) is as follows: eluting 1.5-2.5 column volumes with water, eluting 4-6 column volumes with 15-25% ethanol aqueous solution, and eluting 8-12 column volumes with 45-55% ethanol aqueous solution.
  2. 2. The method according to claim 1, wherein the temperature of the mixing treatment with the enzyme in the step (1) is 40 to 55 ℃ and the time is 1 to 3 h.
  3. 3. The method of claim 1, wherein the temperature of the negative pressure cavitation extraction in step (1) is 45-55 ℃ and the time is 1.5-2.5 h.
  4. 4. The method of claim 1, wherein the negative pressure cavitation extraction in step (1) has a negative pressure intensity of 70-100 mbar.
  5. 5. The method of claim 1, wherein the number of extractions in step (1) is at least two.
  6. 6. The method of claim 1, wherein the macroporous resin of step (2) comprises AB-8 or D101.
  7. 7. The method of claim 6, wherein the macroporous resin of step (2) is AB-8.
  8. 8. An extract of dulcamara produced by the process of any one of claims 1-7.
  9. 9. Use of the extract of dulcamara according to claim 8 for the preparation of food, health products or cosmetics.

Description

Duying extract of Yunnan Tibetan and preparation method and application thereof Technical Field The invention belongs to the technical field of natural product extraction and preparation, and particularly relates to a herba Duchesneae Indicae extract and a preparation method and application thereof, in particular to a herba Duchesneae Indicae extract with high geraniin content and a preparation method and application thereof. Background Yunnan Tibetan Du Yingshi Equisetum genus tall trees of Equisetum genus of Equisetum family, produced in Yunnan and Tibet, growing in evergreen forest with the altitude of 1300-3000 m. Other plants of the genus Duying, such as Duying, etc., have been studied to some extent at present, but studies on Duying of Yunnan Tibetan at home and abroad have been focused on population distribution and species identification, and there are few studies on its extracts and its activity. CN114796172a discloses an active site of a mountain dulcamara branch and leaf extract with acetylcholinesterase inhibitory activity and application thereof, belonging to the technical field of plant medicines. The invention extracts the dried branches and leaves of mountain dulcamara with solvent to obtain crude extract PXS48-1A, prepares ethyl acetate extraction part PXS48-3A and n-butanol extraction part PXS48-4A by extracting PXS48-1A, and respectively finely divides the ethyl acetate part PXS48-3A and n-butanol extraction part PXS48-4A to obtain six active parts such as PXS48-23, PXS48-24, PXS48-25, PXS48-43, PXS48-44 and PXS 48-45. The invention also provides a pharmaceutical composition composed of the plant medicine active site of the mountain dulcamara branch and leaf, a preparation method of the plant medicine active site and the pharmaceutical composition, and application of the plant medicine active site and the pharmaceutical composition in preparing medicines for treating Alzheimer's disease. CN114504602a discloses the use of extract of dulcamara calycarpa in the preparation of H +-K+ -atpase inhibitor, and its use in the preparation of medicament for preventing or treating stomach related diseases, in particular, the use of extract of dulcamara calycarpa in the preparation of medicament for treating gastric ulcer, and the preparation method of extract of dulcamara calycarpa. CN103467533a discloses a method for extracting dulcis from oval mountain dulcis folium. The preparation method comprises pulverizing folium Elaeagni Angustifoliae, adding appropriate amount of water and biological enzyme, performing enzymolysis for 5-10 hr, adding 5-10 times of 80-99% ethanol solution, reflux extracting for 2-3 times, recovering ethanol under reduced pressure, concentrating, diluting with appropriate amount of water, filtering, ultrafiltering with ultrafiltration membrane, concentrating the filtrate with nanofiltration membrane, collecting concentrated solution, adding appropriate amount of gelatin solution, precipitating, dissolving the precipitate with acetone solution, collecting acetone solution recovery reagent, and vacuum drying. The invention has the advantages of simple process operation, low energy consumption, little pollution and easy realization of industrial production. However, the method only researches other species of dulcamara, and the extraction process and effect of the dulcamara are not related at all. Therefore, how to provide a method for effectively extracting the dulcamara and researching the technical effects of related extracts is a problem to be solved. Disclosure of Invention Aiming at the defects of the prior art, the invention aims to provide a herba violae Yunnanensis extract and a preparation method and application thereof, in particular to a herba violae Yunnanensis extract with high geraniin content and a preparation method and application thereof. The method provided by the invention can effectively extract active substances in the Duying of the Yunnan Tibetan, and the obtained Duying extract has the advantages of high content of geraniin, high content of total flavone, low content of macromolecular tannins such as Du Yingrou, good oxidation resistance effect, high hyaluronidase inhibition activity, good anti-inflammatory effect, high collagenase activity inhibition rate, economy and environmental protection, and the used reagent can be recycled and can be used as an active substance in health care products. In order to achieve the aim of the invention, the invention adopts the following technical scheme: in one aspect, the present invention provides a method for preparing an extract of dulcamara, the method comprising the steps of: (1) Pulverizing herba Duchesneae Indicae, mixing with enzyme, and extracting with solvent under negative pressure to obtain extract of herba Duchesneae Indicae Du Yingcu; (2) And (3) adsorbing and eluting the extract of the Yunnan Tibetan Du Yingcu obtained in the step (1) by using macroporous resin to obtain the extract of the Yunnan Tibeta