CN-118437056-B - Positive pressure filtering method and device

CN118437056BCN 118437056 BCN118437056 BCN 118437056BCN-118437056-B

Abstract

The present disclosure provides a positive pressure filtration method and apparatus, the positive pressure filtration method comprising obtaining a range of pressure values of different liquids during a peptide fragment desalting process; setting a plurality of filtering pressures according to the pressure value range, setting a plurality of filtering times according to the filtering pressures, and switching the filtering pressures in the filtering column according to the filtering times to carry out gradient filtering on the solution. According to the positive pressure filtering method, the original fixed pressure value and the fixed time are split into the pressure gradient with multiple steps consisting of different pressure values and different pressure times, and different circulation times are added, so that the problem of inconsistent flow velocity when liquid passes through the filter plate can be well solved.

Inventors

JIANG LUN
WU RONGZONG
CHEN XIAOXUAN

Assignees

上海奔曜科技有限公司

Dates

Publication Date: 20260508
Application Date: 20240603

Claims (10)

1. A positive pressure filtration method comprising: obtaining the pressure value range of different liquids in the process of desalting the peptide fragment; Setting a plurality of filtering pressures according to the pressure value range; Setting a plurality of corresponding filtering times according to a plurality of the filtering pressures; and switching the filtering pressure in the filtering column according to the filtering time to carry out gradient filtering on the solution.
2. The positive pressure filtration method of claim 1, wherein setting a plurality of filtration pressures according to the range of pressure values comprises: at least setting a first filtering pressure and a second filtering pressure, wherein the first filtering pressure corresponds to a first filtering time, and the second filtering pressure corresponds to a second filtering time, and the first filtering pressure is larger than the second filtering pressure.
3. The positive pressure filtration method of claim 2, wherein setting a plurality of filtration pressures according to the range of pressure values further comprises: setting a third filtering pressure, wherein the third filtering pressure corresponds to a third filtering time, and when the first filtering pressure and the second filtering pressure act, if liquid is retained in part of the filtering columns, switching the third filtering pressure, the first filtering pressure and the second filtering pressure in sequence to carry out next round of filtering, wherein the third filtering pressure is not smaller than the second filtering pressure.
4. The positive pressure filtration method of claim 3, wherein obtaining a range of pressure values for the different liquids during the peptide fragment desalting process comprises: the pressure value range of different medium solutions in different material filter columns is tested.
5. The positive pressure filtration method according to claim 4, wherein the first filtration time t 1 is equal to or less than 0.1s equal to or less than 1 is equal to or less than 0.3s, the second filtration time t 2 is equal to or less than 5s equal to or less than 2 is equal to or less than 60s, and the third filtration time t 3 is equal to or less than 0.1s equal to or less than 2 is equal to or less than 10s.
6. The positive pressure filtration method according to claim 1 or 5, wherein gradient filtering the solution according to the filtration pressure in the filtration column switched according to the filtration time comprises: and performing gradient filtration on the solution according to the filtration pressure in the filtration column which is circularly switched according to the filtration time.
7. The positive pressure filtration method of claim 6, wherein gradient filtering the solution according to the filtration pressure in the filtration column is switched according to the filtration time cycle comprises cycling at least twice.
8. A positive pressure filtration device, comprising: The positive pressure module is used for adjusting and maintaining the pressure in the filtering column so as to enable the solution to flow through the sub-filtering column to filter impurities in the solution, and the positive pressure module is used for carrying out positive pressure filtering by the following method: obtaining the pressure value range of different liquids in the process of desalting the peptide fragment; Setting a plurality of filtering pressures according to the pressure value range; filtering the solution in a filter column according to the filtration time by switching the filtration pressure; The support assembly is arranged below the positive pressure module and is used for placing the filter column; The driving assembly is connected with the positive pressure module and used for driving the positive pressure module to move towards the direction close to or far away from the filtering column.
9. The positive pressure filter device according to claim 8, wherein the positive pressure module comprises an upper positive pressure bin and a lower positive pressure bin, an air inlet end is arranged on the upper positive pressure bin, a positive pressure hole is arranged at the bottom of the lower positive pressure bin, a sealing gasket is arranged on the end face of the bottom of the lower positive pressure bin, and a through hole corresponding to the positive pressure hole is arranged on the sealing gasket.
10. The positive pressure filter apparatus of claim 9 wherein the drive assembly comprises a platen and a drive member connected to both ends of the platen, the upper positive pressure cartridge being secured to the platen, the drive member comprising a lead screw and a motor.

Description

Positive pressure filtering method and device Technical Field The disclosure relates to the technical field of filtration, in particular to a positive pressure filtration method and device. Background Positive pressure filtration devices are commonly used in small pharmaceutical plants, food and beverage, factory and mine hospitals, laboratories, and the like for filtering liquids, clarifying, sterilizing, and biologically purifying. The principle of the positive pressure filter device is that the filter module is placed in a high-pressure positive pressure chamber to complete the filtering operation so as to improve the filtering efficiency. The traditional positive pressure module can realize that peptide solution after proteolysis flows through a part of filter column/membrane by keeping a fixed pressure value and time so as to achieve the effect of filtering impurities in the peptide solution. However, the fixed pressure value and time are difficult to ensure that the flowing-through speed of the peptide solution in all the filtering columns is the same or close, so that incomplete removal of impurities in the peptide solution can be caused, the obtained peptide eluent has uneven volume and other adverse results when the peptide solution is eluted, meanwhile, the liquid in the individual filtering columns is already flowing-through due to the non-uniformity of the liquid discharging speed, a plurality of non-flowing-through filtering columns exist, at the moment, air can escape into the flowing-through filtering columns, the liquid in the rest non-flowing-through filtering columns can not continue to flow downwards, and the phenomenon can also cause the non-uniformity of the final result. Disclosure of Invention In order to solve the above problems, it is necessary to provide a positive pressure filtration method and apparatus, which can better solve the problem of inconsistent flow rate when the liquid passes through the filter plate by dividing the original fixed pressure value and fixed time into multiple steps of pressure gradients composed of different pressure values and different pressure times and assisting different circulation times. According to one aspect of the present application, there is provided a positive pressure filtration method comprising: obtaining the pressure value range of different liquids in the process of desalting the peptide fragment; Setting a plurality of filtering pressures according to the pressure value range; Setting a plurality of corresponding filtering times according to a plurality of the filtering pressures; and switching the filtering pressure in the filtering column according to the filtering time to carry out gradient filtering on the solution. In some embodiments, setting the plurality of filter pressures according to the range of pressure values includes setting at least a first filter pressure corresponding to a first filter time and a second filter pressure corresponding to a second filter time, wherein the first filter pressure is greater than the second filter pressure. In some embodiments, setting the plurality of filter pressures according to the pressure value range further includes setting a third filter pressure, wherein the third filter pressure corresponds to a third filter time, and when the first filter pressure and the second filter pressure act, if a part of the filter columns have liquid retained therein, switching the third filter pressure, the first filter pressure and the second filter pressure in sequence to perform next round of filtration, wherein the third filter pressure is not smaller than the second filter pressure. In some embodiments, obtaining a range of pressure values for different liquids during the peptide fragment desalting process includes testing a range of pressure values for different media solutions in different material filter columns. In some embodiments, the first filtering time t 1 satisfies 0.1 s≤t 1≤0.3 s, the second filtering time t 2 satisfies 5 s≤t 2≤60 s, and the third filtering time t 3 satisfies 0.1 s≤t 2≤10 s. In some embodiments, gradient filtering the solution according to the filtration pressure in the filtration time-switched filtration column comprises gradient filtering the solution according to the filtration pressure in the filtration time-cycled-switched filtration column. In some embodiments, gradient filtering the solution according to the filtration pressure in the filtration column is switched according to the filtration time cycle comprises cycling at least twice. According to another aspect of the present application, there is provided a positive pressure filtration device comprising: The positive pressure module is used for adjusting and maintaining the pressure in the filtering column so as to enable the solution to flow through the sub-filtering column to filter impurities in the solution, and the positive pressure module is used for carrying out positive pressure filtering by the followi