CN-118440977-B - SlCHP16 gene and application of over-expression vector thereof in tomato planting

Abstract

The invention relates to the technical field of genetic engineering, and particularly discloses SlCHP gene and application of an over-expression vector thereof in tomato planting, wherein a cDNA sequence of the SlCHP gene is shown as SEQ ID No.1, and the tomato root system growth can be obviously promoted and the salt and alkali stress resistance of tomatoes can be obviously enhanced after the over-expression vector is constructed by utilizing the SlCHP gene to transform tomato plants. The SlCHP gene provided by the invention constructs an over-expression vector, which is beneficial to promoting the growth of tomato root systems and enhancing the salt and alkali stress resistance of tomatoes.

Inventors

• LI GUOBIN
• HU XIAOHUI
• KANG ZHEN
• LI JIANMING
• PAN JIAQI
• CHEN GUO
• SUN CHANG
• LI ZHENGLUN

Assignees

• 西北农林科技大学

Dates

Publication Date

20260505

Application Date

20240516

Claims (4)

1. The application of the SlCHP16 gene in tomato planting is characterized in that the cDNA sequence of the SlCHP gene is shown as SEQ ID No.1, and the SlCHP gene is used for promoting the growth of tomato root systems after constructing an over-expression vector to transform tomato plants, so that the salt-alkali stress resistance of tomatoes is enhanced.
2. 2. A method for promoting tomato root system development and improving salt and alkali stress tolerance by using SlCHP gene over-expression vector, which is characterized by comprising the following steps: Cloning SlCHP genes in tomatoes, constructing an over-expression vector, transferring tomatoes by using agrobacterium infection after agrobacterium transformation, culturing to obtain SlCHP T0 transgenic plants with over-expression of SlCHP, and selecting single plants for selfing to obtain T1 transgenic plants; the growth speed of primary roots of T1 generation transgenic plant seedlings is increased, the length of root tip cells is increased, the growth of root systems is accelerated, and the salt tolerance is enhanced.
3. The application of the overexpression vector of the SlCHP16 gene or bacterial liquid in tomato planting is characterized in that the bacterial liquid is obtained by transforming competent cells with the overexpression vector of SlCHP genes, the transgenic tomato plant is obtained by transfecting tomato plants after transforming agrobacterium with the overexpression vector of SlCHP genes, and the total length of the obtained transgenic tomato plant root system and the total surface area of the root system are increased.
4. 4. Use according to claim 3, characterized in that the transgenic tomato plants obtained by transformation of tomato plants with an overexpression vector have an increased resistance to salt and alkaline stress.

Description

SlCHP16 gene and application of over-expression vector thereof in tomato planting Technical Field The invention relates to the technical field of genetic engineering, in particular to SlCHP gene and application of an overexpression vector thereof in tomato planting. Background Tomato (solanum lycopersicum ml.) is one of the important vegetable crops in our country, the perennial cultivation area exceeds 100 ten thousand hm 2, and the yield of fresh tomatoes is stable in the first world. However, tomatoes are susceptible to a variety of abiotic stresses throughout the growth cycle, as a moderately salt-sensitive plant, the yield and quality of which are severely limited by salt-alkali stress. With the continuous deterioration of the global environment and improper fertigation measures, the salinization of soil becomes an important environmental problem which restricts the continuous and high-quality development of the vegetable industry. As one of vegetable crops with the largest facility planting area, the growth and development of tomatoes are seriously affected by saline-alkali stress, so that fruits are reduced, yield is reduced, and synthesis and accumulation of soluble proteins, organic acids, lycopene, vitamin C and the like in the tomatoes are affected. In nature, salt stress and alkali stress often accompany, and the combined action constitutes saline-alkali stress, which is much more harmful to plants than any single stress. The high salt and high pH environment of plant rhizosphere under saline-alkali stress can cause plants including osmotic stress, unbalanced nutrition level, ion specific toxicity and oxidative damage, and inhibit various physiological activities of plants. The root system of the plant plays a vital role in the growth, development and survival processes of the plant, and the functions of the plant comprise water and nutrient absorption, plant body stabilization and the like, and when the plant is stressed by saline and alkaline, the root system of the plant is firstly influenced, so that the growth of the plant is slow, and then the development of the overground part is influenced. Under the stress of salt and alkali, the length, diameter, surface area, volume and other morphological indexes of the root system of horticultural crops such as tomatoes, peppers, melons and the like are obviously affected, the root system is changed from milky white to brown, the activity is reduced, and even death is caused when serious. The DC1 (DIVERGENT C1) domain protein was originally found in animals and was one domain of the lipid binding module of protein kinase C (protein kinase Cs, PKCs). Some DC1 domain proteins found in plants at present relate to the processes of plant disease resistance, stress resistance, growth and development and the like. DC1 domain proteins are reported to be involved in plant resistance to infection by pathogenic bacteria in tobacco and capsicum respectively. DC1 domain protein is found in wheat and cotton to raise the tolerance of plant to salt stress. There are 21 and DC1 domain proteins in tomato, but their role in tomato growth development and stress response has not been studied. SlCHP16 has higher expression in root system and is subjected to saline-alkali stress response, so that the SlCHP gene is researched and the roles and functions of the SlCHP gene in tomato growth and saline-alkali stress response are clarified, stress response and growth and development cooperative regulation factors are discovered, and the method has very important significance for cultivating new saline-alkali resistant tomato varieties, developing new measures for saline-alkali resistant cultivation of vegetable crops and realizing efficient and sustainable development of modern agriculture. Disclosure of Invention In order to improve the salt tolerance of tomatoes, the invention provides SlCHP genes and application of the over-expression vector in tomato planting, and the SlCHP genes provided by the invention construct the over-expression vector, which is beneficial to promoting the growth of tomato root systems and enhancing the salt and alkali stress resistance of tomatoes. The invention provides an application of SlCHP gene in tomato planting, wherein the cDNA sequence of SlCHP gene is shown as SEQ ID No.1, and the SlCHP gene is used for promoting the growth of tomato root system after constructing an over-expression vector to transform tomato plants, so that the salt-alkali stress resistance of tomatoes is enhanced. The invention also provides an over-expression vector of SlCHP gene, which is obtained by connecting SlCHP gene fragment with PHELLSGATE vector. The invention also provides a construction method of the SlCHP gene over-expression vector, which comprises the following steps: Using tomato cDNA as a template and using a sequence shown as SEQ ID No.2-3 as a primer to amplify and obtain SlCHP gene fragments; the obtained SlCHP gene fragment and the linearized PHELLSGA