CN-118441053-B - Application of PIP4K2C gene in breast cancer detection

Abstract

The invention belongs to the technical field of gene diagnosis and treatment, and particularly relates to application of a PIP4K2C gene in breast cancer detection, wherein the kit comprises a solid phase carrier and a detection reagent coated on the solid phase carrier, and the detection reagent is used for detecting the PIP4K2C gene or PIP4K2C protein. The PIP4K2C gene and the protein expression product thereof can be used as specific markers for diagnosing breast cancer, and the small interfering RNA designed aiming at the PIP4K2C gene can also be used as a gene therapy tool for preparing and treating the breast cancer, thereby providing a novel breast cancer therapy approach.

Inventors

• ZHONG XIAOLI
• JIN YUE
• LI XINYUE

Assignees

• 苏州检力康科技有限公司

Dates

Publication Date

20260508

Application Date

20240607

Claims (1)

1. The application of siRNA in preparing a product for inhibiting growth, proliferation or invasion of breast cancer cells is characterized in that the siRNA is a specific siRNA designed for PIP4K2C genes, and the nucleotide sequence of the siRNA is shown as follows in the specification of sense:5'-UAGGAUUCAUUCUCCAUGUAA-3' and anti-sense:5'-AUCCUAAGUAAGAGGUACAUU-3'.

Description

Application of PIP4K2C gene in breast cancer detection Technical Field The invention belongs to the technical field of gene diagnosis and treatment, and particularly relates to application of PIP4K2C genes in breast cancer detection. Background Breast cancer is a heterogeneous malignant tumor, accounts for about 30% of all female cancers, is one of the most common malignant tumors of women at home and abroad, and is also a main cause of female cancer-related death. In recent years, with the development and deep research of medicine, diagnosis and treatment of breast cancer have made important progress. Breast cancer, however, is still frequently subject to tumor recurrence and metastasis, with high levels of malignancy and mortality. At present, diagnosis of breast cancer in clinic mainly depends on pathological detection, and effective specific molecular markers, especially molecular markers for early breast cancer diagnosis, are lacking. Therefore, finding new tumor markers has important scientific and clinical significance. Disclosure of Invention Aiming at the defects of the prior art, the invention provides application of PIP4K2C genes in breast cancer detection, and the PIP4K2C genes can be used as a first pathological molecular marker for effectively distinguishing normal breast epithelial cells from cancerous epithelial cells. The technical scheme provided by the invention is as follows: a kit for breast cancer detection, the kit comprising a solid support and a detection reagent coated on the solid support, the detection reagent being for detecting PIP4K2C gene or PIP4K2C protein. Further, the kit comprises reagents for performing immunohistochemical quantitative detection on PIP4K2C protein and corresponding labels or instructions. Further, the kit comprises reagents for quantitative detection of PIP4K2C gene mRNA and corresponding labels or instructions. Further, the kit comprises PIP4K2C specific primers and self-made PIP4K2C specific antibodies. Further, the PIP4K2C proteins include fusion proteins and non-fusion proteins. Further, the label or the instruction of the kit comprises that when PIP4K2C antibody immunohistochemical staining of normal tissues around the detected object shows (0 to+), and the breast epithelium staining of the suspicious region shows (+ ++ to+++), the possibility of canceration of the under-lens area is obviously increased; invasive breast cancer epithelial cell staining the significance is enhanced (+++ to++++), suggesting a significantly increased likelihood of infiltration of cancer in the under-the-lens region. Further, the label or the instruction book of the kit comprises that when the ratio of the mRNA expression quantity of PIP4K2C relative to the reference protein of the detection object to the mRNA expression quantity of PIP4K2C relative to the reference protein of the tissue beside the cancer is more than or equal to 0.5, the probability of the detection object suffering from breast cancer is higher than that of the common crowd. Further, the breast tissue sample comprises breast cancer tissue and paracancerous tissue. Further, the tag or instructions of the kit include that the reference protein includes GAPDH. The invention also provides application of the PIP4K2C protein in preparing a gene therapy tool for inhibiting growth or proliferation of breast cancer cells or preparing a medicament for treating breast cancer. The invention also provides application of the PIP4K2C gene or PIP4K2C protein detection reagent in preparation of a reagent or a kit for detecting breast cancer. The invention also provides a method for non-therapeutically inhibiting the growth, proliferation or invasion of breast cancer cells in vitro, which comprises the step of designing specific siRNA for PIP4K2C to transfect the breast cancer cells, thereby inhibiting the growth, proliferation or invasion of the breast cancer cells. Further, the nucleotide sequence of the designed specific siRNA for PIP4K2C is shown as follows: sense:5'-UAGGAUUCAUUCUCCAUGUAA-3', anti-sense:5'-AUCCUAAGUAAGAGGUACAUU-3'. Further, transfection reagents used to transfect breast cancer cells include liposomes. Advantageous effects 1. Through extensive and intensive studies, it was found that the gene PIP4K2C may be an effective molecular marker for diagnosis of breast cancer. The PIP4K2C gene codes for the protein PIP4K gamma, which belongs to the family of phosphatidylinositol-5-phosphate 4-kinases (PIP 4K) with PIP4K alpha and PIP4K beta. It can produce specific PI (4, 5) P2 by phosphorylating phosphatidylinositol 5-phosphate (PI 5P), which is important for peroxisome function, cholesterol transport, and tumorigenesis. The expression level of PIP4K2C in breast cancer cells was significantly increased compared to normal breast epithelial cells. PIP4K2C is also significantly highly expressed in breast cancer tissue relative to paracancerous tissue, and therefore PIP4K2C can be an important marker for patholog