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CN-118476488-B - Animal behavioural detection device and method based on infrared laser light sheet

CN118476488BCN 118476488 BCN118476488 BCN 118476488BCN-118476488-B

Abstract

The invention relates to an animal behavior detection device and method based on an infrared laser film, wherein the detection device comprises a test bed module, a light path module, an imaging module and a control module, the test bed module comprises a hole which is communicated up and down and a light hole, the hole is used for placing an animal to be tested, the light path module adopts a Bawil prism structure, one-dimensional laser generated by the light path module is aligned with the light hole, when the animal behavior detection device is used, the light hole is filled with the one-dimensional laser generated by the light path module, a first camera of the imaging module is used for recording reflection signal changes, a second camera is used for recording animal behaviors by using a spectroscope, and the control module is connected with the first camera and the second camera and used for sending trigger signals to the first camera and the second camera and then recording image data transmitted by the first camera and the second camera for subsequent experimental analysis. The device has simple structure and is flexible and changeable. The parameters such as the wavelength of the light source, the height of the cushion layer and the like can be changed according to the characteristics of experimental animals.

Inventors

  • SUN YI
  • LI ZHOU
  • NING JING
  • ZHANG XINGJIANG

Assignees

  • 西湖大学

Dates

Publication Date
20260505
Application Date
20240604

Claims (9)

  1. 1. An animal behavior detection device based on an infrared laser light sheet is characterized by comprising a test bed module (1), an optical path module (2), an imaging module (3) and a control module (4), The test bed module (1) comprises a top plate (11), a bottom plate (12), a middle plate (13) and a cushion layer (14), The top plate (11), the middle plate (13), the cushion layer (14) and the bottom plate (12) are sequentially paved from top to bottom, the top plate (11) and the bottom plate (12) are made of transparent materials, the middle plate (13) is opaque, holes (131) penetrating up and down are formed in the middle plate (13), the holes (131) are used for placing animals to be tested, the cushion layer (14) is provided with light holes, and the holes (131) are communicated with the light holes; The light path module (2) adopts a Bawil prism structure, and can uniformly distribute the Gaussian-distributed collimated light beams (27) again to form a layer of uniform light sheets (28), and when the animal behavior detection device is used, the uniform light sheets (28) generated by the light path module (2) are filled in the light holes; The imaging module (3) comprises a first camera (31), a second camera (32) and a spectroscope (33), wherein the first camera (31) is positioned below the bottom plate (12) and is parallel to the bottom plate (12), the second camera (32) is positioned below the bottom plate (12) and is perpendicular to the bottom plate (12) and is positioned right below the hole (131), the spectroscope (33) is positioned below the bottom plate (12) and is positioned right below the hole (131), the spectroscope (33) is also positioned at the intersection of the first camera (31) and the route of the second camera (32), the first camera (31) is used for recording reflective signal changes, and the second camera (32) records animal behaviors by using the spectroscope (33); the control module (4) is connected with the first camera (31) and the second camera (32) and is used for sending trigger signals to the first camera (31) and the second camera (32), and then recording image data returned by the first camera (31) and the second camera (32) for subsequent experimental analysis.
  2. 2. The animal behavior detection device based on the infrared laser light sheet according to claim 1, wherein the top plate (11) is a transparent acrylic plate, the bottom plate (12) is a transparent inorganic glass, and the middle plate (13) is a milky acrylic plate so as to shield the laser light source.
  3. 3. An animal behavioural detection device based on infrared laser light sheets as claimed in claim 1, characterized in that the thickness of the middle plate (13) is such that the animal to be detected can move within the hole (131), said animal being a small insect.
  4. 4. The device for detecting animal behaviors based on the infrared laser light sheet according to claim 1, wherein four cushion layers (14) are arranged and are respectively positioned at four corners of the bottom plate (12), the cushion layers (14) are made of frosted glass paper, the thickness of the cushion layers is 0.1mm, and light holes with the thickness of 0.1mm are formed.
  5. 5. The animal behavior detection device based on the infrared laser light sheet according to claim 1, wherein the light path module (2) adopts a Bowell prism structure, and comprises an infrared light source (21), a laser (22), a triaxial steering micro-operation platform (23), a biaxial steering micro-operation platform (24), a Bowell prism (25) and a steering micro-operation platform (26), The laser device comprises a laser (22), a light source (21), a test bench module (1), a Bowell prism (25), a light sheet (28) and an infrared light source (21), wherein the laser (22) is arranged on a triaxial steering micro-operation platform (23), the steering micro-operation platform (26) is arranged in front of the laser (22), the steering micro-operation platform (26) is arranged on a biaxial steering micro-operation platform (24), the Bowell prism (25) is arranged on the steering micro-operation platform (26), the Bowell prism (25) is used for uniformly distributing Gaussian-distributed collimated light beams (27) generated by the laser (22) again and expanding the Gaussian-distributed collimated light beams into the light sheet (28) in a layer, and the infrared light source (21) is arranged above the test bench module (1) and is used for providing a light source for an imaging module (3).
  6. 6. The animal behavioral detection device based on an infrared laser light sheet according to claim 5, wherein the steering micro-operation platform (26) comprises a first bracket (261) and a second bracket (262), the first bracket (261) is connected to the two-axis steering micro-operation platform (24) through a connecting rod (263), the powell lens (25) is fixed to the second bracket (262), the second bracket (262) is connected to the first bracket (261), a screw hole (264) is formed in the first bracket (261), a fine tuning bolt (265) matched with the screw hole (264) is formed in the second bracket (262), and the connection between the second bracket (262) and the first bracket (261) is realized through the connection between the fine tuning bolt (265) and the screw hole (264).
  7. 7. The device according to claim 1, wherein the control module (4) comprises a host (41), a display (42), a keyboard (43), a mouse (44) and a junction box (45), the host (41) is respectively connected with the display (42), the keyboard (43), the mouse (44) and the junction box (45), the junction box (45) is further connected with the first camera (31) and the second camera (32), a trigger signal is sent to the first camera (31) and the second camera (32) by using the junction box, and image data returned by the two cameras is transmitted to the host (41) for subsequent experimental analysis.
  8. 8. An animal behavioural detection method based on an infrared laser light sheet, characterized in that it is performed based on the animal behavioural detection device as claimed in any one of claims 1-7, comprising the steps of: S1, placing a bottom plate (12), a cushion layer (14) and a middle plate (13), placing an animal to be detected, placing a top plate (11), and assembling an experiment table module; S2, adjusting the position of the light path module (2), and starting a laser light source; S3, starting the imaging module (3) and the control module (4), and triggering the imaging module (3) to shoot the behavior of the animal to be detected; s4, analyzing behavioral data of the animal to be detected by the control module (4).
  9. 9. The animal behavioral testing method based on infrared laser light sheet according to claim 8, wherein after the light path module and the experiment table module are matched, the uniform light sheet passes through the light hole of the experiment table, so that far infrared light is distributed in a space which is 0.1mm away from the bottom plate (12) during the experiment of the animal, when any body part of the animal enters the far infrared light area, strong reflection is formed, the reflection signal and the real-time behavior of the animal are recorded by the imaging module, one camera records the reflection signal change, and the other camera records the behavior of the animal by using the spectroscope.

Description

Animal behavioural detection device and method based on infrared laser light sheet Technical Field The invention belongs to the technical field of animal behavioural detection, and particularly relates to an animal behavioural detection device and method based on an infrared laser sheet. Background Animal behavioural research can be used in a plurality of fields such as drug test, psychological research, neurobiological research and the like, and promotes a significant discovery of life science. However, many animal behavior experiments can cause irreversible wounds to experimental animals, which can influence experimental results to a certain extent and also bring challenges to experimental ethics. In addition, in animal behavioural experiments, a plurality of behavioural tests are required, including whether behaviours occur, duration of behaviours, degree of progress, etc., and all these indexes need to be quantified. In neurobiological research, scientists often quantify the behavior of experimental animals by means of various methods, however insects have difficulty in behavior detection due to the characteristics of small head, rapid behavior and the like. Chinese patent CN208064278U discloses a semi-open wind tunnel device for research of insect behavior and chemical ecology, which belongs to the field of research of insect behavior and ecology, and comprises a wind tunnel main body frame which is formed by enclosing a wind tunnel left side wall, a wind tunnel right side wall, a wind tunnel front wall, a wind tunnel rear wall and a wind tunnel bottom wall into a semi-closed frame structure, wherein an air inlet is arranged on the wind tunnel front wall, a wind source is arranged at the front end of the air inlet, an air outlet is arranged on the wind tunnel rear wall, an insect throwing platform is arranged on the wind tunnel main body frame, a tubular outer insect cage is arranged on the insect throwing platform, the lower end of the outer insect cage is arranged in a cavity of the wind tunnel main body frame, an insect outer outlet is arranged at the lower end of the side wall of the outer insect cage, and a control member for opening and closing the insect outlet is arranged on the outer insect cage. The device is characterized in that an odor source is placed in a wind tunnel main body frame, flowing air is arranged in the wind tunnel main body frame, and a more natural experimental space is provided. However, the device is a small wind tunnel and does not have an optical detection function. Chinese patent CN208925005U discloses a novel device for testing olfactory behaviors of small moth insects, which comprises a miniature air pump, wherein the air outlet end of the miniature air pump is in butt joint with an activated carbon filter column through an air duct, the air outlet end of the activated carbon filter column is in butt joint with a gas flowmeter through the air duct, the air outlet end of the gas flowmeter is in butt joint with a water filter column through the air duct, the air outlet end of the water filter column is in butt joint with a tee joint through the air duct, and the other two ends of the tee joint are in butt joint through an air duct behavior testing device. The device can simultaneously test 2030 the behavioral response of the test insects to the odor substances to be tested, the working efficiency is obviously improved, the cold light source incandescent lamp ensures that the care intensity of the test insects in the movable chamber in all directions is always, and the influence of the difference of light and shade on the movement orientation of the test insects can be effectively avoided, thereby influencing the test insects to make behavioral selection on different odor stimuli. However, the device only tests the olfactory behaviors of the moth insects, and cannot detect whether other behavior details (such as eating, exploring and gait change) are changed or not. Disclosure of Invention Based on the current situation that devices capable of flexibly detecting animal behavior details (such as feeding, probing, gait change) and the like are lacking in the prior art, the invention provides an animal behavior detection device and method based on an infrared laser light sheet. The aim of the invention can be achieved by the following technical scheme: the invention provides an animal behavior detection device based on an infrared laser sheet, which comprises a test bed module, a light path module, an imaging module and a control module, The test bed module comprises a top plate, a bottom plate, a middle plate and a cushion layer, The top plate, the middle plate, the cushion layer and the bottom plate are sequentially paved from top to bottom, the top plate and the bottom plate are made of transparent materials, the middle plate is opaque, holes which are communicated up and down are formed in the middle plate and are used for placing animals to be tested, and light holes a