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CN-118576656-B - Camellia oleifera seed meal extract, extraction method thereof and application of camellia oleifera seed meal extract in preparation of anti-colon cancer drugs

CN118576656BCN 118576656 BCN118576656 BCN 118576656BCN-118576656-B

Abstract

The invention provides a camellia oleifera meal extract, an extraction method thereof and application thereof in preparing anti-colon cancer drugs, and relates to the technical field of medicines. According to the extraction method provided by the invention, by performing heat treatment on the oil tea meal before extraction, active ingredients in the oil tea meal extract can be effectively regulated and controlled, and the sensitivity and inhibition effect on colon cancer cells are improved.

Inventors

  • LI WENJUAN
  • LUO YIPING
  • LI WENJIE
  • YAO YUFEI
  • PENG XIAOPING
  • CHEN XUANYING

Assignees

  • 南昌大学

Dates

Publication Date
20260512
Application Date
20240617

Claims (9)

  1. 1. A method for extracting an oil tea dreg extract applied to an anti-colon cancer drug is characterized by comprising the following steps of carrying out heat treatment on oil tea dreg powder for 5-7 hours at 150-170 ℃, leaching and separating leaching liquor in a leaching reagent, concentrating under reduced pressure to obtain an extract, dissolving the extract in chloroform, extracting for multiple times by using saturated sodium bicarbonate aqueous solution, merging to obtain an extract, acidifying the extract at 0+/-2 ℃ to pH 2-3, extracting for multiple times by using chloroform, merging an organic layer to obtain an extract, concentrating under reduced pressure and drying the extract to obtain the oil tea dreg extract, wherein the leaching reagent is 80-95vol% ethanol aqueous solution.
  2. 2. The extraction method according to claim 1, wherein the number of times of extraction is 2 to 5 when the extraction is performed with a saturated sodium bicarbonate solution and/or the number of times of extraction is 2 to 5 when the extraction is performed with chloroform.
  3. 3. The extraction method according to claim 1, wherein when the oil tea meal powder is subjected to heat treatment at 150-170 ℃, the average particle size of the oil tea meal powder is 0.40-0.50mm, and/or the passing rate of the oil tea meal powder under a 40-mesh screen is 100%.
  4. 4. The extraction method according to claim 1, wherein the process of leaching and separating the leaching liquor in the leaching agent comprises mixing the heat-treated oil tea meal powder with the leaching agent in a volume ratio of 1 (15-25), leaching at 20-30 ℃ for 36-60h, and suction filtering to separate the leaching liquor.
  5. 5. The extraction method according to claim 1, wherein the extraction reagent is recovered when concentrating under reduced pressure to obtain an extract, and/or chloroform is recovered when concentrating under reduced pressure the extract.
  6. 6. An oil tea meal extract extracted by the extraction method of any one of claims 1 to 5.
  7. 7. Use of the oil tea meal extract extracted by the extraction method according to any one of claims 1 to 5 for preparing a medicament for treating colon cancer.
  8. 8. The use according to claim 7, wherein the oil tea meal extract promotes apoptosis by increasing intracellular ROS content of colon cancer, and/or wherein the oil tea meal extract promotes apoptosis by decreasing intracellular SOD activity of colon cancer, and/or wherein the oil tea meal extract promotes apoptosis by increasing intracellular MDA content of colon cancer, and/or wherein the oil tea meal extract promotes apoptosis by decreasing intracellular mitochondrial membrane potential of colon cancer, and/or wherein the oil tea meal extract promotes apoptosis by increasing intracellular mitochondrial ROS of colon cancer.
  9. 9. The use of claim 8, wherein the colon cancer cells are HCT-116 cells.

Description

Camellia oleifera seed meal extract, extraction method thereof and application of camellia oleifera seed meal extract in preparation of anti-colon cancer drugs Technical Field The invention relates to the technical field of medicines, in particular to a camellia oleifera dreg extract, an extraction method thereof and application thereof in preparing medicines for resisting colon cancer. Background Colon cancer is the third most common malignancy worldwide, and causes of poor diet, obesity, alcoholism, smoking, and physical exercise inactivity increase the risk of colon cancer in the population. By 2040 years, there are expected to be a new 192 tens of thousands of cases of colon cancer worldwide, and the burden will further increase in the next few years. Methods of treatment of colon cancer have been expanded with the accumulation of clinical practice, including chemotherapy, surgery, radiation therapy, and the like. However, current strategies for chemotherapy of colon cancer suffer from a number of drawbacks, particularly some of the side effects of the drug after repeated administration. More and more studies have shown that drugs produced from natural products can be used in various ways to treat colon cancer, and that pharmaceutical phytochemicals can be used in vitro and in vivo to treat or prevent colon cancer. Therefore, the development of drugs with anti-colon cancer effect is very urgent and important for the prevention and treatment of colon cancer. Disclosure of Invention The invention aims to provide an oil tea meal extract, an extraction method thereof and application thereof in preparing anti-colon cancer drugs, and active ingredients in the oil tea meal extract can be effectively regulated and controlled by performing heat treatment before extracting the oil tea meal, so that the sensitivity and the inhibition effect on colon cancer cells are improved. In a first aspect, the invention provides an extraction method of an oil tea meal extract applied to preparation of an anti-colon cancer drug, wherein the oil tea meal is subjected to heat treatment for 5-7 hours at 150-170 ℃ before being extracted. According to the invention, before the oil tea meal is extracted, the active ingredients in the oil tea meal can be effectively regulated and controlled by heat treatment, and more phenolic substances and organic acids are obtained, so that the sensitivity and toxicity of colon cancer cells can be improved, and the inhibition effect of colon cancer cells can be effectively improved. Optionally, the preparation method comprises the steps of carrying out heat treatment on oil tea meal powder at 150-170 ℃ for 5-7 hours, leaching and separating leaching liquor in a leaching reagent, concentrating under reduced pressure to obtain extract, dissolving the extract in chloroform, carrying out multiple extractions by using saturated sodium bicarbonate aqueous solution, merging to obtain extract, acidifying the extract at 0+/-2 ℃ until the pH value of the extract is 2-3, carrying out multiple extractions by using chloroform, merging to obtain extract, concentrating under reduced pressure, and drying to obtain the oil tea meal extract. Alternatively, when the extraction is performed several times using saturated sodium bicarbonate solution, the number of extractions is 2-5 times. Alternatively, when chloroform is used for multiple extractions, the number of extractions is 2-5. Optionally, the leaching agent includes at least one of an aqueous 80-95vol.% ethanol solution and methanol. Optionally, when the oil tea meal powder is subjected to heat treatment at 150-170 ℃, the average particle size of the oil tea meal powder is 0.40-0.50mm. Optionally, when the oil tea meal powder is subjected to heat treatment at 150-170 ℃, the passing rate of the oil tea meal powder under a 40-mesh screen is 100%. Optionally, the process of leaching and separating the leaching liquor in the leaching reagent comprises the steps of mixing the heat-treated oil tea meal powder with the leaching reagent according to the volume ratio of 1:15-25, leaching for 36-60h at 20-30 ℃ and then leaching and separating the leaching liquor. Optionally, the leaching agent is recovered when concentrating under reduced pressure to obtain an extract. Optionally, chloroform is recovered when the extract is concentrated under reduced pressure. In a second aspect, the invention also provides an oil tea meal extract extracted by any one of the above optional extraction methods. In a third aspect, the invention also provides application of the oil tea meal extract extracted by any one of the optional extraction methods in preparation of anti-colon cancer drugs. In fact, the oil tea meal extract promotes apoptosis by increasing the intracellular ROS content of colon cancer. In fact, the oil tea meal extract promotes apoptosis by reducing the intracellular SOD activity of colon cancer. In fact, the oil tea meal extract promotes apoptosis by increasing the i