Search

CN-118577288-B - Cu-MoS with controllable catalytic capability and morphology2Nanometer enzyme and preparation method and application thereof

CN118577288BCN 118577288 BCN118577288 BCN 118577288BCN-118577288-B

Abstract

The invention discloses a Cu-MoS 2 nano enzyme with adjustable catalytic capability and morphology, a preparation method and application thereof. The invention adopts a solvothermal method, and prepares Cu-MoS 2 nano enzyme capable of realizing atomic-level dispersion in DMF solution by (NH 4 ) 2 MoS 4 and Cu coordination. The adjustable Cu content enables the product to show adjustable peroxidase-like activity, superoxide dismutase-like activity and glutathione oxidase-like activity. Meanwhile, the introduction of Cu enables catalytic sites on a material substrate to be more abundant, the whole catalytic process is transferred to Cu sites, and the peroxidase-like activity of Cu-MoS 2 is improved. In addition, the nano enzyme has good antibacterial property, and the antibacterial rate of the nano enzyme to staphylococcus aureus can reach 99% when the concentration of H 2 O 2 is 1mM and the concentration of a sample is 50 mu g/mL, thereby being hopeful to provide an effective solution for the continuous challenge of bacterial infection.

Inventors

  • LI ZHIRU
  • MENG ZIXUAN
  • SHEN JIAHAO

Assignees

  • 陕西科技大学

Dates

Publication Date
20260505
Application Date
20240523

Claims (6)

  1. 1. A method for realizing regulation and control of catalytic activity and morphology of Cu-MoS 2 nano enzyme by adjusting copper content is characterized in that a mixed solution is obtained by adding a DMF solution of CuCl 2 into a DMF solution of (NH 4 ) 2 MoS 4 ), carrying out ultrasonic treatment and stirring uniformly, carrying out solvothermal reaction on the mixed solution in an autoclave at 180-220 ℃ for 18-24 hours, centrifuging, separating a product, and washing to obtain Cu-MoS 2 nano enzyme; When the molar ratio of copper to molybdenum is 0.1-0.5:1, the Cu-MoS 2 nano-enzyme presents a nano-rod structure formed by self-assembled nanoflower, and when the molar ratio of copper to molybdenum is 0.7-0.9:1, the Cu-MoS 2 nano-enzyme presents a hollow nano-box structure assembled by the nanoflower; When the molar ratio of copper to molybdenum is 0.3:1, the prepared Cu-MoS 2 nano-enzyme has peroxidase-like activity, when the molar ratio of copper to molybdenum is 0.7:1, the prepared Cu-MoS 2 nano-enzyme has superoxide dismutase-like activity, and when the molar ratio of copper to molybdenum is 0.9:1, the prepared Cu-MoS 2 nano-enzyme has glutathione oxidase-like activity.
  2. 2. The method for realizing the regulation and control of the catalytic activity and morphology of the Cu-MoS 2 nano-enzyme by adjusting the content of copper according to claim 1, wherein the DMF solution of (NH 4 ) 2 MoS 4 ) is a DMF solution of (NH 4 ) 2 MoS 4 , ultrasonic 10min, stirring 30 min) with 3-4mg/mL (NH 4 ) 2 MoS 4 ) added into DMF.
  3. 3. The method for regulating and controlling the catalytic activity and morphology of the Cu-MoS 2 nano-enzyme by adjusting the content of copper according to claim 1, wherein the DMF solution of CuCl 2 is a DMF solution obtained by adding CuCl 2 into a sufficient amount of DMF solution capable of completely dissolving CuCl 2 and stirring the solution with ultrasound.
  4. 4. The method for realizing the regulation and control of the catalytic activity and morphology of the Cu-MoS 2 nano-enzyme by adjusting the content of copper according to claim 1, wherein the autoclave is a polytetrafluoroethylene-lined stainless steel autoclave.
  5. 5. The method for realizing the regulation and control of the catalytic activity and morphology of the Cu-MoS 2 nano-enzyme by adjusting the content of copper according to claim 1, wherein the reaction filling rate of the solvothermal reaction is 50-60%.
  6. 6. The method for controlling the catalytic activity and morphology of the Cu-MoS 2 nano-enzyme by adjusting the copper content according to claim 1, wherein the centrifugation is to separate the product by centrifugation at 10000rpm for 5 minutes and washing with deionized water and ethanol three times.

Description

Cu-MoS 2 nano enzyme with controllable catalytic capability and morphology, and preparation method and application thereof Technical Field The invention belongs to the technical field of nano materials, relates to novel nano mimic enzyme, and in particular relates to Cu-MoS 2 nano enzyme with adjustable catalytic capability and morphology, a preparation method and application thereof. Background In recent years, with the rapid development of nano technology, nano enzyme is increasingly introduced into the sight of people, and has wide development prospect because of various enzyme-like characteristics, adjustable enzyme-like properties and physicochemical stability, and can be used in various fields of biosensors, cancer treatment, antibacterial application, chemical synthesis and the like. MoS 2, a two-dimensional transition metal disulfide, has attracted great attention in this field, its lower band gap, can make full use of the solar spectrum from UV to Vis to generate photo-generated electron-hole pairs, and can also use infrared light to generate heat. As nanoenzymes, moS 2 has been demonstrated to possess a variety of mimic enzyme catalytic properties, such as catalase-like (CAT-like) activity, peroxidase-like (POD-like) activity, superoxide dismutase-like (SOD-like) activity, and glutathione oxidase-like (GSHOXD-like) activity. However, one major limitation of MoS 2 is that its catalytic activity is mainly concentrated at the edges and the (002) basal plane of MoS 2 is almost catalytically inert, which limits the application of MoS 2, and overcoming these limitations is the focus of recent innovations. Patent document CN115779965A introduces polydopamine as a linking bridge between MoS 2 and Cu nanoparticles in a preparation method of MoS 2 -based nano-enzyme, so that small-size Cu nanoparticles can be uniformly dispersed on the surface of MoS 2, preparation of MoS 2 nanosheets is combined with Cu nanoparticles, the preparation method is relatively complex, and there are problems that doping process is difficult to effectively dope metal into lattice, proportion of introduced elements cannot be accurately controlled, and the like. Disclosure of Invention The invention aims to provide Cu-MoS 2 nano enzyme with adjustable catalytic capability and morphology, which can solve the problems that in the prior art, metal is difficult to dope into a crystal lattice effectively, the proportion of introduced elements cannot be controlled accurately and the like, and a preparation method and application thereof. In order to achieve the aim, the technical scheme is that a mixed solution is obtained by adding a DMF solution of CuCl 2 into a DMF solution of (NH 4)2MoS4) according to a copper-molybdenum molar ratio of 0.1-0.9:1, and then carrying out ultrasonic treatment and stirring uniformly, the mixed solution is subjected to solvothermal reaction in an autoclave at 180-220 ℃ for 18-24 hours, and then products are centrifugally separated and washed to obtain the Cu-MoS 2 nano enzyme with adjustable catalytic capability and morphology. The DMF solution of (NH 4)2MoS4) is obtained by adding (NH 4)2MoS4, ultrasonic 10min and stirring 30 min) to DMF to obtain 3-4mg/mL DMF solution of (NH 4)2MoS4). The DMF solution of CuCl 2 is prepared by adding CuCl 2 to a sufficient amount of DMF solution capable of completely dissolving CuCl 2 and stirring the solution with ultrasound to obtain a DMF solution of CuCl 2. The autoclave was a polytetrafluoroethylene-lined stainless steel autoclave. The reaction filling rate of the solvothermal reaction is 50-60%. The centrifugation was carried out by centrifugation at 10000rpm for 5 minutes to isolate the product and washing with deionized water and ethanol three times. The Cu atoms of the Cu-MoS 2 nano enzyme are uniformly distributed on the MoS 2 base in an atomic level, and the activity of the nano enzyme is regulated and controlled by regulating the content of copper. When the molar ratio of copper to molybdenum is 0.1-0.5:1, the Cu-MoS 2 nano-enzyme presents a nano-rod structure formed by self-assembled nanoflower, and when the molar ratio of copper to molybdenum is 0.7-0.9:1, the Cu-MoS 2 nano-enzyme presents a hollow nano-box structure assembled by the nanoflower. When the molar ratio of copper to molybdenum is 0.3:1, the prepared Cu-MoS 2 nano-enzyme has peroxidase-like activity, when the molar ratio of copper to molybdenum is 0.7:1, the prepared Cu-MoS 2 nano-enzyme has superoxide dismutase-like activity, and when the molar ratio of copper to molybdenum is 0.9:1, the prepared Cu-MoS 2 nano-enzyme has glutathione oxidase-like activity. The Cu-MoS 2 nano enzyme with adjustable catalytic capability and morphology has an antibacterial performance, and when the molar ratio of copper to molybdenum is 0.2-0.4:1, the antibacterial effect is obvious, and the antibacterial effect can be improved by adding H 2O2. Compared with the prior art, the invention has the following beneficial e