CN-118592640-B - Pickering emulsion containing menthol and preparation method thereof

Abstract

The invention belongs to the technical field of foods, and particularly relates to a Pickering emulsion containing menthol and a preparation method thereof. The Pickering emulsion containing menthol is prepared by taking menthol and cyclodextrin composite particles as solid particle emulsifying agents and soybean oil as an oil phase, wherein the mass ratio of the menthol to the cyclodextrin is 1:2-5. The Pickering emulsion containing menthol prepared by the invention has good storage stability and rheological property within 30 days, and in addition, the emulsion also has excellent oxidation resistance and antibacterial property, and in an in-vitro simulated digestion experiment, the release rate of free fatty acid of the emulsion in small intestine is 76.64+/-1.08%, and the bioavailability of menthol is improved while the emulsion has good slow release property.

Inventors

• HE JINXING
• YANG MINGHE

Assignees

• 齐鲁工业大学(山东省科学院)

Dates

Publication Date

20260512

Application Date

20240422

Claims (1)

1. 1. The preparation method of the Pickering emulsion containing menthol is characterized in that the Pickering emulsion is prepared by taking menthol and beta-cyclodextrin composite particles as solid particle emulsifying agents and soybean oil as an oil phase; The preparation method of the Pickering emulsion containing menthol comprises the following steps: S1, preparation of menthol and beta-cyclodextrin composite particles Taking 10g of beta-cyclodextrin, dispersing the beta-cyclodextrin in 80g of distilled water with the water temperature of 45 ℃ to prepare a suspension, then slowly adding 3g of menthol into the suspension while stirring, continuing stirring and reacting for 3 hours at the temperature of 45 ℃ after the menthol is added, preserving the obtained suspension in a refrigerator at the temperature of 5 ℃ for 24 hours, standing and precipitating, filtering, washing and precipitating with absolute ethyl alcohol, and finally, freeze-drying to obtain composite particle powder of the menthol and the beta-cyclodextrin; s2, preparation of Pickering emulsion Dissolving the menthol and beta-cyclodextrin composite particle powder obtained in the step S1 in water to prepare a menthol and beta-cyclodextrin composite particle water solution with the mass volume fraction of 3%, then mixing the menthol and beta-cyclodextrin composite particle water solution with soybean oil with the volume fraction of 60%, and homogenizing the mixture at 20000rpm for 3min to finally obtain the Pickering emulsion containing menthol; The Pickering emulsion can fully exert the bacteriostatic effect of menthol, has good slow-release effect on menthol, and has a release rate of 76.64% of free fatty acid after simulated in-vitro digestion for 2 hours.

Description

Pickering emulsion containing menthol and preparation method thereof Technical Field The invention belongs to the technical field of foods, and particularly relates to a Pickering emulsion containing menthol and a preparation method thereof. Background Menthol (C 10H20 O, (1 r,2s,5 r) -2-isopropyl-5-methylcyclohexanol) is a naturally occurring chiral volatile cyclic terpene alcohol, mainly extracted from the peppermint plant Mentha x piperita (la Mi Yake), with a representative peppermint smell. Menthol is unstable in nature, sensitive to light, heat and oxygen, is susceptible to loss by degradation and volatilization, and has a certain irritation, so that many potential applications thereof are severely limited. In order to improve the stability of menthol and slow down the degradation and volatilization loss of menthol, the prior research adopts a micro-nano carrier embedding technology to protect and utilize the menthol, for example Zhu et al take hydroxypropyl cyclodextrin as a main body and menthol as an object, and successfully prepare an inclusion compound, but the release rate of the inclusion compound is limited by temperature and has higher release rate at 279.1 ℃, in addition, patent CN112705131A discloses a eutectic solvent/hydroxypropyl-beta-cyclodextrin menthol microcapsule, and hexadecyl trimethyl ammonium chloride is added into the microcapsule to improve the stability of the microcapsule. In addition, in other studies, inclusion compounds were prepared between starch and menthol of different amylose content, the highest mass fraction of menthol in the inclusion compound was only 5.5% even if the adsorption time exceeded one day. Pickering emulsions as a green substitute for traditional surfactant stabilized emulsions can utilize lower colloidal particle loadings to achieve effective emulsion stabilization. The pickering emulsion is stabilized by solid particles rather than surfactants, and because of the irreversible adsorption of the solid particles at the oil-water interface, a dense barrier can be formed, so the stability of pickering emulsion is superior to that of traditional emulsion. Cyclodextrin (CD) is a series of cyclic oligosaccharides which are generated by acting Cyclodextrin glucosyltransferase on glucose polymers such as starch, maltose, glycogen and the like and have unique externally hydrophilic and internally hydrophobic molecular structures, so that the Cyclodextrin has excellent embedding capability, and can protect some active substances which are easy to be oxidized and decomposed through inclusion, for example, catechin/beta-Cyclodextrin inclusion compounds are prepared by a saturated aqueous solution method such as Sha and the influence of the inclusion compounds on an oral instant film is explored. In addition, cyclodextrin is used as embedding material to prepare active ingredient into pickering emulsion, and the obtained product has the advantages of both, for example, patent CN113519822A discloses a temperature-responsive cyclodextrin nanoparticle pickering emulsion, and cyclodextrin is used for embedding sensitive ingredients such as quercetin, retinol, lycopene and the like, so that the pickering emulsion with temperature-responsive cyclodextrin nanoparticle is finally obtained, however, the pickering emulsion prepared by the method of the patent has obvious temperature change. At present, the research direction of menthol at home and abroad mainly comprises the development of mint essence and mint essential oil, and the method has no effective and widely available load path for the characteristics of unstable property and easy volatilization of menthol. The Pickering emulsion can be used as a good carrier to load menthol, so that the menthol is converted from a crystal structure into an emulsion form, and the Pickering emulsion has wide application value. Disclosure of Invention In order to solve the technical problems, the invention provides a Pickering emulsion containing menthol and a preparation method thereof. The Pickering emulsion containing menthol is prepared by taking menthol and cyclodextrin composite particles as solid particle emulsifiers and soybean oil as an oil phase, wherein the mass ratio of the menthol to the cyclodextrin is 1:2-5. Preferably, the cyclodextrin is selected from any one of alpha-cyclodextrin, beta-cyclodextrin and gamma-cyclodextrin. More preferably, the cyclodextrin is beta-cyclodextrin. In fact, cyclodextrin molecular inclusion technology has been applied in the related art and can effectively improve the solubility and stability of functional active ingredients, however, the main research direction of the prior menthol embedding technology is to use a compound or embed peppermint essential oil, one of which has the problem of limited application range, and the other kind of compound contained in peppermint essential oil is more, so compared with other technologies, menthol crystals can be directly embedded in the form of em