CN-118595153-B - Application of microorganism bacterium for repairing soil pollution

Abstract

The invention relates to the technical field of microorganisms, in particular to application of microorganism bacteria for repairing soil pollution. The strain used in the invention is Trichoderma harzianum 124D, which is a broad-spectrum biocontrol microbial inoculum, and can obviously reduce copper accumulation in soil and relieve the stress influence on wheat growth.

Inventors

• YE DUNWEI
• LI LING
• Zhang Hezun
• LIU KAIQUAN
• Zhao Runer
• MA HANXU
• YANG XINJIE
• ZHANG YANYI
• HOU JUNYAO

Assignees

• 齐鲁工业大学(山东省科学院)

Dates

Publication Date

20260505

Application Date

20240702

Claims (4)

1. 1. The application of the trichoderma harzianum 124D in the soil pollution remediation is characterized in that the preservation number of the trichoderma harzianum 124D is CGMCC No.21030; The method for repairing soil pollution comprises the steps of relieving stress influence of copper on plant growth and reducing copper accumulation in soil, wherein the copper content in the soil is more than 500mg/kg; the plant is wheat; The Trichoderma harzianum 124D is specifically Trichoderma harzianum 124D spore liquid, and the spore amount in the Trichoderma harzianum 124D spore liquid is more than or equal to 1 multiplied by 10 6 CFU/mL.
2. 2. The method of claim 1, wherein the growth indicator comprises a germination characteristic of wheat and a seedling growth characteristic of wheat.
3. 3. The use according to claim 2, wherein the wheat germination characteristics include germination rate, germination vigor, sprout length, root length.
4. 4. The use according to claim 2, wherein the wheat seedling stage growth characteristics include dry weight biomass, plant height, SOD total activity, POD activity, malondialdehyde content.

Description

Application of microorganism bacterium for repairing soil pollution Technical Field The invention belongs to the technical field of microorganisms, and particularly relates to application of microorganism bacteria for repairing soil pollution. Background The disclosure of this background section is only intended to increase the understanding of the general background of the invention and is not necessarily to be construed as an admission or any form of suggestion that this information forms the prior art already known to those of ordinary skill in the art. In recent decades, the copper ion content in the soil environment is improved by the large-scale application of copper preparation pesticides and copper-containing fertilizers, and the copper content of the soil after a plurality of long-term application of copper preparation pesticides is seriously exceeded, so that the physicochemical properties of the soil are changed, the microbial population and biomass are seriously affected, and the plant death is caused by excessive copper. Several types of methods for restoring copper contaminated environments have been tried to date, all of which suffer from various degrees of limitations. Many of the conventional treatment methods are mostly treated by chemical agents, so that unpleasant odors are easily generated, and the treatment methods are high in cost and have adverse effects on the environment. Thus, the problem of copper pollution is an environmental protection problem that needs to be solved. Therefore, the application prospect of the technology means based on biological ecology, such as plant repair technology and microorganism repair technology, becomes particularly clear. Trichoderma is widely used as a widely existing soil-dwelling fungus and an active ingredient of a biological pesticide and a fertilizer, and is required to have good environmental tolerance in combination with a chemical pesticide and a fertilizer. Particularly in copper contaminated soil or in combination with copper formulated pesticides, good copper tolerance is required. Patent CN112501033A discloses a Trichoderma harzianum 124D with a collection number of CGMCC No.21030, and antagonism experiments show that the Trichoderma harzianum 124D has good antagonism to a plurality of pathogenic bacteria such as phytophthora infestans, pythium ultimum, wheat Brucella powdery mildew, fusarium graminearum and the like, and can promote the yield increase of wheat mu, improve the protein and amino acid content of wheat caryopsis and further improve the resistance of wheat. The functions of the light emitting diode are still required to be researched to expand the application range of the light emitting diode. Disclosure of Invention In order to solve the problems, the invention provides an application of microorganism bacteria for repairing soil pollution. The invention further supplements the function of the Trichoderma harzianum 124D, the Trichoderma harzianum 124D can relieve the stress influence of copper on the growth of wheat, promote the resistance of the wheat and promote the absorption and transportation of the wheat to copper, and has important significance for restoring the copper polluted environment. In order to achieve the above purpose, the present invention adopts the following technical scheme: In a first aspect of the invention, the application of trichoderma harzianum 124D in soil pollution remediation is provided, wherein the preservation number of the trichoderma harzianum 124D is CGMCC No.21030. In some embodiments, the remediation of soil pollution includes alleviating the stress effects of copper on plant growth and/or reducing copper accumulation in the soil. In some embodiments, the plant is wheat. In some embodiments, the growth indicator comprises a wheat germination characteristic, a wheat seedling growth characteristic. In some embodiments, the wheat germination characteristics include germination rate, germination vigor, sprout length, root length. In some embodiments, the wheat seedling stage growth characteristics include dry weight biomass, plant height, SOD total activity, POD activity, malondialdehyde content. In some embodiments, the copper content in the soil is greater than 500mg/kg. In a second aspect, the invention provides a microbial inoculum for restoring soil pollution, which comprises trichoderma harzianum 124D spore liquid. In some embodiments, the preparation method of the Trichoderma harzianum 124D spore liquid comprises the steps of picking up Trichoderma harzianum 124D, inoculating the Trichoderma harzianum 124D into a sterilized culture solution, and obtaining a trichoderma 124D suspension after shaking culture. In some embodiments, the amount of spores in the Trichoderma harzianum 124D spore solution is greater than or equal to 1X 10 6 CFU/mL. The beneficial effects of the invention are that (1) The biocontrol bacterium used in the invention is Trichoderma harzianum 124D, which is a bioc