CN-118638675-B - Fibrillated fiber micro-bacteria and application thereof

Abstract

The invention discloses a fibrillated fiber microorganism and application thereof. The fibrillated fiber micro-bacteria are named IURM R and Cellulosimicrobium cellulans, and are preserved in China general microbiological culture Collection center (CGMCC) with the preservation number of 29729 in the year 1 and 23 of 2024. The fibrillated fiber micro-bacteria provided by the invention have high-efficiency ammonia nitrogen degradation performance under the high-salt condition. The optimal degradation temperature of the fiber-made fiber micro-bacteria to ammonia nitrogen is 30-35 ℃, and the degradation effect to ammonia nitrogen is best under the condition that the acidity and the alkalinity are neutral. The fibrillated fiber microbacterium provided by the invention has low C/N tolerance and wide C/N tolerance range, so that the fibrillated fiber microbacterium has high-efficiency nitrogen removal capability.

Inventors

• REN JIANJUN
• RAN YUNDE
• LIU JUN
• WANG ZHONGQING
• LI CHUNYU
• LIU YU
• PAN XINGLIANG
• ZHANG WEN

Assignees

• 常州大学
• 北京市畜牧总站
• 山西晟禾生物科技有限公司

Dates

Publication Date

20260505

Application Date

20240530

Claims (7)

1. 1. The fibrillated fiber microbacterium is named IURM R and Cellulosimicrobium cellulans in taxonomy, and is preserved in China general microbiological culture Collection center (CGMCC) No.29729 in the year 2024 and the month 1 and 23.
2. 2. A preparation for degrading a nitrogen-containing compound, comprising the fibrillated cellulose micro-bacterium according to claim 1, wherein the nitrogen-containing compound comprises an ammonia salt and/or a nitrate salt.
3. 3. Use of the fibrillated cellulose micro-bacterium according to claim 1 or the nitrogen containing compound degrading formulation according to claim 2 in a denitrification process, characterized in that the nitrogen containing wastewater comprises ammonia nitrogen and/or nitrate nitrogen.
4. 4. The use according to claim 3, wherein the denitrification treatment temperature is 30-35 ℃.
5. 5. The use according to claim 3, wherein the denitrification treatment is carried out at a pH of 6 to 9.
6. 6. The use according to claim 3, wherein the denitrification treatment is carried out under acidic and basic neutral conditions.
7. 7. The method according to claim 3, wherein the denitrification treatment has a C/N of 1 to 5.

Description

Fibrillated fiber micro-bacteria and application thereof Technical Field The invention relates to the technical field of water treatment and environmental microorganism, in particular to a fibrillated fiber microorganism and application thereof. Background With the continuous deep progress of town and industrialization, a great amount of domestic sewage and industrial wastewater containing high-concentration nitrogen-containing organic matters are discharged into a water area system, the excessive discharge of nitrogen and phosphorus elements is an important factor causing water eutrophication and water pollution, and the problem of water pollution is also a sustainable development severely restricting related entity industries. The emission of a large amount of nitrogen elements not only can pollute drinking water source sites and threaten drinking water safety of people and livestock, but also can reduce dissolved oxygen in the water body, aggravate eutrophication of the water body, destroy aquatic ecology, harm aquatic organism inhabitation and reproduction, release pungent odor to cause black and odorous water body, and further influence human health. The treatment of nitrogen-containing wastewater mainly comprises a physical/chemical method and a biological method. The physical/chemical or biotechnological treatment methods are costly and require additional energy. The microbial method is gradually popularized due to the advantages of mild and efficient action conditions, strong economy, no secondary pollution and the like. Because ammonia oxidizing bacteria are not salt-tolerant, the ammonia oxidizing bacteria have the characteristics of slow reaction, low denitrification rate and large oxygen demand, the treatment efficiency of the ammonia oxidizing bacteria on salt-containing wastewater is not high, and the nitrogen-containing wastewater is difficult to treat effectively. Therefore, the screening of the safe and salt-tolerant denitrifying bacteria has important application prospect in the application of nitrogenous wastewater. Disclosure of Invention Aiming at the defects in the prior art, the invention provides a fibrillated fiber micro-bacterium and an application thereof in denitrification treatment. In order to achieve the above purpose, the technical scheme adopted by the invention is as follows: In one aspect, the invention provides a fibrillated fiber microbacterium, named IURM R, taxonomic Cellulosimicrobium cellulans, deposited in China general microbiological culture Collection center (CGMCC) at 1-23 of 2024, with a deposit number of CGMCC No.29729. In yet another aspect, the present invention provides a nitrogen-containing compound degrading formulation comprising the above-described fibrillated cellulose micro-bacterium. Preferably, the nitrogen-containing compound comprises an ammonia salt and/or a nitrate salt. In yet another aspect, the present invention provides the use of the above-described fibrillated fibrous microorganisms or the above-described nitrogen-containing compound degrading formulation in denitrification treatment. Preferably in the denitrification of nitrogen-containing wastewater. Preferably, the nitrogen-containing wastewater comprises ammonia nitrogen and/or nitrate nitrogen. Preferably, the temperature of the denitrification treatment is 30-35 ℃. Preferably, the denitrification treatment is performed under a pH of 6 to 9, preferably under a neutral pH. Preferably, the C/N of the denitrification treatment is 1-5. The technical scheme has the following advantages or beneficial effects: the invention obtains the fibrous fiber micro bacteria IURM R and 23 through soil screening, and the bacteria have good ammonia nitrogen degradation capability and higher high-concentration salt tolerance capability, and can be used for treating ammonia nitrogen in high-salt wastewater. Meanwhile, the strain has degradation capability on ammonia nitrogen in different environments, and the degradation rate of the ammonia nitrogen in pig farm manure water reaches 75.26 percent. Drawings FIG. 1 is a plate colony pattern and a microscopic pattern of a fibrillated fibrous micro bacterium IURM R in example 1 of the present invention. FIG. 2 is a tree of the molecular evolution of the fibrillated fiber microorganisms constructed in example 1 of the present invention. FIG. 3 is a graph showing the effect of the micro-bacteria IURM R to remove ammonia nitrogen from the fiber under different salt concentrations in example 2 of the present invention. FIG. 4 is a graph showing the effect of the micro-bacteria IURM R to remove ammonia nitrogen from the fiber at different temperatures in example 3 of the present invention. FIG. 5 is a graph showing the effect of the fibrillated fiber microorganisms IURM R on ammonia nitrogen removal at different pH values in example 3 of the present invention. FIG. 6 is a graph showing the effect of the micro-bacteria IURM R to remove ammonia nitrogen from the fiber