CN-118652812-B - Endophytic bacillus beleiensis and application thereof

Abstract

The invention discloses endophytic bacillus belgium and application thereof, and relates to the technical field of biological control. The invention has good control effect on the black currant alternaria leaf spot. The endophytic bacillus belicus is preserved in China general microbiological culture collection center (CGMCC) with the preservation number of 30625. In the field control, when the OD600 value of the biocontrol strain SH1 fermentation liquid is 0.5, the control effect reaches 75.7 percent. When the concentration of lipopeptide extract of biocontrol strain SH1 is 0.5 mug/mL, the control effect reaches 80.8%, bacillus beidelensis SH1 has good biocontrol potential in the control of alternaria melanogaster leaf spot, and the application research should be further enhanced.

Inventors

• LIU JINXIN
• LI YONGGANG
• ZHANG XUE
• WANG SHUNI
• HE YING
• QIN DONG
• HUO JUNWEI

Assignees

• 东北农业大学

Dates

Publication Date

20260505

Application Date

20240711

Claims (4)

1. 1. The endophytic bacillus beleiensis is characterized in that the endophytic bacillus beleiensis (Bailus velezensis) is preserved in China general microbiological culture Collection center (CGMCC) with the preservation number of CGMCC No. 30625.
2. 2. A biocontrol microbial agent comprising the endophytic bacillus bailii of claim 1.
3. 3. Use of endophytic bacillus besii according to claim 1 for controlling alternaria alternata in black currant, characterized in that the alternaria alternata in black is initiated by ALTERNARIA ALTERNATA.
4. 4. Use of the endophytic bacillus beijerinckii of claim 1 for controlling a plant foliar disease pathogen, said plant foliar disease pathogen being any one of Diaporthe eres、Sphaerulina azaleae、Colletotrichum acericola、Corynespora cassiicola、Neopestalotiopsis rosae and ALTERNARIA ALTERNATA.

Description

Endophytic bacillus beleiensis and application thereof Technical Field The invention relates to the technical field of biological control, in particular to endophytic bacillus behenicus and application thereof. Background Blackcurrants are one of the dominant tree species in cold areas of China, and the current national planting area is about 2 ten thousand mu with the beginning of the Heilongjiang province. The black currant fruit is sweet and sour, has faint scent, can be processed into products such as beverage, fruit wine, essence and the like, and has important nutritive, healthy, medical and cosmetic values. However, with the continuous increase of the planting area and the year of the black currants, leaf spot diseases of the black currants are more serious, so that a large number of leaves and fruits fall off, the quality of the black currants is affected, the yield is reduced, the pathogenic factors of the black currants are not clear, the control effect is quite unsatisfactory, and the black currants become one of the important problems to be solved urgently in the industrial development of the black currants. At present, the pathogenic bacteria which can cause black currant leaf spot are mainly the curvularia curriculum (Pseadopeziza ribis), the curvularia curriculum (Gloesporium ribis) and the curvularia curriculum (Septoria ribis). However, the black currant planting area presents a leaf spot disease which is serious in hazard and has not been reported yet. In gardening test stations at the northeast agricultural university, the affected leaves were found to be initially water immersed and later discolored to light grey or off-white with dark brown irregular spots. Severe cases can curl and wither off, affecting fruit yield and quality. Sun et al (2021) used primers ITS, GAPDH and ACT to identify Alternaria alternata causing Zingiberaceae leaf spot. Li Peiqian et al (2023) used primers ITS, TEF-1 alpha and RPB2 to identify Alternaria alternata that caused Paeonia erythema. Qi et al (2024) used primers ITS, GAPDH, ACT and TEF-1 alpha et al to identify Alternaria alternata that caused dandelion leaf spot. Therefore, providing a biocontrol strain for controlling blackcurrant plant diseases is a problem to be solved by the person skilled in the art. Disclosure of Invention In view of the above, the invention provides an endophytic bacillus beijerinus and application thereof. In order to achieve the above purpose, the present invention adopts the following technical scheme: an endophytic bacillus beleiensis which is preserved in China general microbiological culture Collection center (CGMCC) No.30625,2024, no. 05 month 14 and is preserved in North Star Xiyu No. 1, no.3 of the Korean area of Beijing city, and classified and named bacillus beleiensis and Bailus velezensis. A biocontrol microbial agent comprising the endophytic bacillus bailii of claim 1. The application of endophytic bacillus behenii in preventing and treating alternaria nikoviae leaf spot. The application of endophytic bacillus belicus in preventing and controlling plant leaf diseases, wherein the leaf disease pathogen comprises any one of snake strawberry leaf spot bacteria, rhododendron leaf spot bacteria, maple sugar leaf spot bacteria, elder leaf spot bacteria, kiwi leaf spot bacteria and delphinium leaf spot bacteria. Compared with the prior art, the invention discloses the endophytic bacillus beijerinus SH1 which has good prevention and treatment effects on alternaria alternata in black currant. In the field control, when the OD600 value of the biocontrol strain SH1 fermentation liquid is 0.5, the control effect reaches 75.7 percent. When the concentration of lipopeptide extract of biocontrol strain SH1 is 0.5 mug/mL, the control effect reaches 80.8%, bacillus beidelensis SH1 has good biocontrol potential in the control of alternaria melanogaster leaf spot, and the application research should be further enhanced. Drawings In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the drawings that are required to be used in the embodiments or the description of the prior art will be briefly described below, and it is obvious that the drawings in the following description are only embodiments of the present invention, and that other drawings can be obtained according to the provided drawings without inventive effort for a person skilled in the art. FIG. 1 is a graph showing the field control effect of biocontrol strain SH1 bacterial suspension on black currant leaf spot; FIG. 2 antagonism of biocontrol strain SH1 against 9 plant foliar disease pathogenic fungi; FIG. 3 gram staining and colony and gram staining of biocontrol strain SH 1; FIG. 4 partial physiological and biochemical characteristics of biocontrol strain SH 1; FIG. 5 is a phylogenetic tree of biocontrol strain SH1 constructed based on the 16S rRNA gene; FIG. 6 field control effect of biocontrol microbial